Antibiofilm and Antibacterial Activity of Pistacia Atlantica against some Pathogenic Bacteria

Document Type : Original Article (s)

Authors

1 Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran

2 Assistant Professor, Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran

3 Assistant Professor, Agricultural and Natural Resources Research Center of Lorestan, Khorramabad, Iran

Abstract

Background: Bacterial infections and the antibiotic resistance of pathogens are the most important challenges faced by researchers and physicians. In this study, the antibacterial and antibiofilm properties of methanol extracts of leaf, fruit, and gall of Pistacia atlantica were evaluated against some of pathogenic bacteria.Methods: Leaf, fruit, and gall of Pistacia atlantica were collected from forests of Lorestan Province, Iran. Antibacterial properties were investigated against Staphylococcus aureus, Bacillus cereus, Enterococcus faecalis, Pseudomonas aeruginosa, and Escherichia coli through the well diffusion method. The microdilution method was used to determine the minimum inhibitory concentration and the minimum bactericidal concentration. The antibiofilm activity of methanol extract was studied in the sub-lethal concentrations on biofilm formation of Staphylococcus aureus and Pseudomonas aeruginosa using microtiter plate method.Findings: The methanol extracts of leaf, fruit, and gall of Pistacia atlantica had significant antibacterial effects against the tested bacteria except Escherichia coli. There was a significant relationship between the antibacterial activity and the extract concentration (P < 0.05). The highest antibacterial effect was observed in the gall methanol extract. The minimum inhibitory concentration and the minimum bactericidal concentration of the methanol extract of leaf, fruit, and gall was 3.0-12.5 and 6.25-25.00 mg/ml, respectively. The methanol extract of Pistacia atlantica gall at a concentration of 6 mg/ml reduced the biofilm production of Staphylococcus aureus and Pseudomonas aeruginosa by 100% and 74%, respectively.Conclusion: The methanol extracts of different parts of Pistacia atlantica had antibacterial effects. In addition, the methanol extract reduced biofilm production of Staphylococcus aureus and Pseudomonas aeruginosa.

Keywords

References

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Journal of Isfahan Medical School
Volume 36, Issue 482 - Serial Number 482
May and June 2018
Pages 594-600

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History

  • Receive Date: 27 January 2018
  • Revise Date: 02 May 2024
  • Accept Date: 06 April 2022

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