Journal of Isfahan Medical School

Journal of Isfahan Medical School

Investigating the Reaction of Antisera against Hydatid Cyst Antigens with the Surface of Breast Cancer (4T1), Melanoma (B16F10), and Normal Spleen Cells Using Flow Cytometry Technique

Document Type : Original Article (s)

Authors
Fereshteh Mohamadi 1
Mahshid Shakibapour 1
Seyedeh Maryam Sharafi 2
Ali Reza Andalib 3
Sepideh Tolouei 4
Hossein Yousofi-Darani 5
1 MSc Student, Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 PhD, Environment Research Center, Research Institute for Primordial Prevention of Non-Communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran
3 Professor, Department of Immunology, Isfahan University of Medical Sciences, Isfahan, Iran
4 PhD, Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
5 Professor, Cancer Prevention Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran
10.22122/jims.v36i499.9859
Abstract
Background: Hydatid cyst is the larval stage of the Echinococcus granulosus worm which is located in the humans and animals viscera. In order to study the anticancer mechanisms of this parasite, in this study the response of antisera against hydatid cyst antigens with tumor cells antigens was investigated using flow cytometry technique.Methods: Antisera against hydatid cyst antigens were prepared in rabbits, and added to the melanoma (B16F10) and breast cancer cell (4T1) lines, and also normal mouse spleen cells. The reaction was then monitored via flow cytometry.Findings: There was no significant difference in the reaction of hydatid cyst antisera (protoscolex, cell wall, and cystic fluid) with the melanoma cell line. However, the reaction of antisera against cyst fluid and cyst wall with breast cancer cells was significantly different. Additionally, the reaction of the antisera against protoscolex, wall, and cyst fluid with mouse spleen cells was less than that of normal rabbit serum (as a negative control).Conclusion: The results show that antisera against cyst wall and fluid react with the breast cancer cell line, but not with melanoma cells. These results confirm the anticancer mechanisms of this parasite. So, it may be possible to use it for selective drug delivery.
Keywords
Flow cytometry
Malignant melanoma
Breast Cancer
Hydatid cyst
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Journal of Isfahan Medical School
Volume 36, Issue 499 - Serial Number 499
September and October 2018
Pages 1191-1196

  • Receive Date 15 April 2018
  • Accept Date 06 April 2022