Evaluation of the Relative Frequency of Positive Cultures of BACTEC, Blood, Cerebrospinal Fluid, and Urine in Infants Admitted to the Neonatal Intensive Care Unit (NICU) and the Department of Neonatal Diseases in Alzahra Hospital, Isfahan, Iran, during th

Document Type : Original Article (s)

Authors

1 Associate Professor, Child Growth and Development Research Center AND Department of Pediatrics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

2 Student of Medicine, Student Research Committee, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: The prevalence of bacteria in the neonatal intensive care unit (NICU) and the department of neonatal diseases is constantly changing, and the empiric practice of antibiotics is not effective due to the resistance of these microorganisms. Therefore, the purpose of this study was to determine the relative frequency of positive cultures of BACTEC, blood, cerebrospinal fluid, and urine in infants admitted to NICU and neonatal diseases section of Alzahra hospital, Isfahan, Iran, during the years 2011-2016.Methods: In this cross-sectional descriptive study, 466 positive-culture samples from 2853 different cultures [blood, urine, cerebrospinal (CSF) fluid, etc.] from infants were evaluated for the type of microorganisms and sensitivity to antibiotics. All the infants have clinical signs of neonatal infection and were admitted to NICU and neonatal diseases section of Alzahra hospital during 2011-2016.Findings: Among BACTEC, blood, CSF, and urine cultures, 15.5%, 9.3%, 6.4%, and 28.6% were positive, respectively. Staphylococcus epidermidis was the most prevalent species in the BACTEC (46.7%), blood (53.1%) and CSF (37.1%) cultures, and Klebsiella pneumoniae was the most prevalent species of urinary cultures (28%). The highest susceptibility in our study population was to co-trimoxazole, clindamycin, amikacin, and oxacillin, respectively.Conclusion: Regarding the results of BACTEC and blood cultures, it is essential to use necessary policies to reduce staphylococcal infections.

Keywords

References

  1. Klinger G, Levy I, Sirota L, Boyko V, Reichman B, Lerner-Geva L. Epidemiology and risk factors for early onset sepsis among very-low-birthweight infants. Am J Obstet Gynecol 2009; 201(1): 38-6.
  2. Morales WJ, Dickey SS, Bornick P, Lim DV. Change in antibiotic resistance of group B streptococcus: Impact on intrapartum management. Am J Obstet Gynecol 1999; 181(2): 310-4.
  3. Schrag SJ, Cutland CL, Zell ER, Kuwanda L, Buchmann EJ, Velaphi SC, et al. Risk factors for neonatal sepsis and perinatal death among infants enrolled in the prevention of perinatal sepsis trial, Soweto, South Africa. Pediatr Infect Dis J 2012; 31(8): 821-6.
  4. Camacho-Gonzalez A, Spearman PW, Stoll BJ. Neonatal infectious diseases: Evaluation of neonatal sepsis. Pediatr Clin North Am 2013; 60(2): 367-89.
  5. Coburn B, Morris AM, Tomlinson G, Detsky AS. Does this adult patient with suspected bacteremia require blood cultures? JAMA 2012; 308(5): 502-11.
  6. Arshad M, Seed PC. Urinary tract infections in the infant. Clin Perinatol 2015; 42(1): 17-28.
  7. Bonadio W, Maida G. Urinary tract infection in outpatient febrile infants younger than 30 days of age: A 10-year evaluation. Pediatr Infect Dis J 2014; 33(4): 342-4.
  8. Barichello T, Fagundes GD, Generoso JS, Elias SG, Simoes LR, Teixeira AL. Pathophysiology of neonatal acute bacterial meningitis. J Med Microbiol 2013; 62(Pt 12): 1781-9.
  9. Beam KS, Laughon MM, Hornik CP, Cohen-Wolkowiez M, Clark RH, Benjamin DK, Jr., et al. Predictors of positive cerebrospinal fluid cultures in infants with bacteremia. Pediatr Infect Dis J 2014; 33(4): 360-5.
  10. Villari P, Sarnataro C, Iacuzio L. Molecular epidemiology of Staphylococcus epidermidis in a neonatal intensive care unit over a three-year period. J Clin Microbiol 2000; 38(5): 1740-6.
  11. Behmadi H, Borji A, Taghavi-Rad A, Soghandi L, Behmadi R. Prevalence and antibiotic resistance of neonatal sepsis pathogens in Neyshabour, Iran. Arch Pediatr Infect Dis 2016; 4(2):e33818.
  12. Oordt-Speets AM, Bolijn R, van Hoorn RC, Bhavsar A, Kyaw MH. Global etiology of bacterial meningitis: A systematic review and meta-analysis. PLoS One 2018; 13(6): e0198772.
  13. Blewitt J. Meningitis part 1: Causes, diagnosis and treatment. British Journal of School Nursing 2010; 5(6): 278-82.
  14. Gupta A. Hospital-acquired infections in the neonatal intensive care unit--Klebsiella pneumoniae. Semin Perinatol 2002; 26(5): 340-5.
Journal of Isfahan Medical School
Volume 36, Issue 501 - Serial Number 501
September and October 2017
Pages 1276-1281

Files

History

  • Receive Date: 01 August 2018
  • Revise Date: 03 May 2024
  • Accept Date: 06 April 2022

Share

How to cite

Statistics