Comparison of Interleukin 17 and Interleukin 22 Inflammatory Cytokines in Peripheral Blood Mononuclear Cells of Children with and without Asthma

Document Type : Original Article (s)

Authors

1 MSc Student, Department of Immunology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

2 PhD Student, Department of Molecular Medicine, School of Medicine AND Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran

3 Associate Professor, Immunology, Asthma and Allergy Institute, Tehran University of Medical Sciences, Tehran, Iran

4 PhD Student, Department of Immunology, School of Medicine, Tarbiat Madares University, Tehran, Iran

5 Assistant Professor, Immunology, Asthma and Allergy Institute, Tehran University of Medical Sciences, Tehran, Iran

6 Professor, Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

7 Department of Immunology, Immunology, Asthma and Allergy Institute, Tehran University of Medical Sciences, Tehran, Iran

8 Associate Professor, Abortion Research Centre, Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

Abstract

Background: Asthma is a reversible chronic inflammatory disease of airways that the effects of inflammatory cytokines in its severity has been proven. This study aimed to compare interleukin 17 (IL17) and interleukin 22 (IL22), two effective cytokines in asthma, children with and without asthma.Methods: In this case-control study, 15 children with asthma and 15 healthy children were included. Peripheral blood mononuclear cells (PBMCs) were isolated from 5 cc of heparinized blood samples, and cultured under stimulation with anti-CD3 and anti-CD28 for 72 hours. Percentage of CD4+IL17+ and CD4+IL22+T cells was assessed by flow cytometry method. Secretion of IL17 and IL22 cytokines was measured using enzyme-linked immunosorbent assay (ELIZA) technique.Findings: There was no significant difference between cases and controls in levels of secreted IL17 and IL22 in supernatant of cultured cells. Moreover, there was no significant difference between patients and healthy group in terms of CD4+IL17+ (P = 0.30) and CD4+IL22+ (P = 0.41) T cells in PBMCs; although the median percentage of IL17+IL22+ T cells in CD4+ T-cell subset had an elevated level in patients group but the difference was not significant (P = 0.56).Conclusion: In studied community containing children with relative- and completely-controlled asthma, there were no significant differences with healthy children in IL17 and IL22 levels, as well as CD4+IL17+ and CD4+IL22+ T cells in PBMCs. Although the median percentage of CD4+IL17+IL22+* T cells had not a significant elevated level in patients group, more studies with larger sample sizes could be helpful.

Keywords

References

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Journal of Isfahan Medical School
Volume 36, Issue 504 - Serial Number 504
September and October 2019
Pages 1376-1381

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History

  • Receive Date: 20 September 2017
  • Revise Date: 06 May 2024
  • Accept Date: 06 April 2022

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