Journal of Isfahan Medical School

Journal of Isfahan Medical School

Evaluation of the Effect of Rosmarinic Acid in Induction of Neural Differentiation in Wharton’s Jelly Stem Cells

Document Type : Original Article (s)

Authors
Mohsen Salmanvandi 1
Shabnam Kermani 2
Akram Alizadeh 3
1 MSc Student, School of Materials Science and Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iran
2 Assistant Professor, School of Materials Science and Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iran
3 Assistant Professor, School of Materials Science and Engineering, Najafabad Branch, Islamic Azad University, Najafabad AND Department of Tissue Engineering and Applied Cell Sciences, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran
10.22122/jims.v37i519.11213
Abstract
Background: Rosemarinic acid is a phenolic compound that has neuroprotective properties. It has beneficial effect in neurological diseases such as Parkinson's and Alzheimer's. Rosmarinic acid increases the expression of neurotrophic factors such as brain-derived neurotrophic factor (BDNF), which can contribute in neuronal differentiation of stem cells.Methods: After isolation and proliferation, Wharton’s jelly stem cells were placed in inductive culture medium containing Rosmarinic acid. MTT assay was used to evaluate toxicity. After 4 days, induction of neuronal differentiation in Wharton’s jelly stem cells was evaluated using real-time polymerase chain reaction (real-time PCR) via analyzing the expression of Nestin and β-Tubulin III genes.Findings: MTT assay showed was no significant difference in viability between cells cultured in culture medium with Rosmarinic acid and control group (P < 0.05). Real-time PCR showed that adding Rosmarinic acid to culture medium increased the expression of Nestin and β-Tubulin III genes (P < 0.05).Conclusion: Increasing the expression of β-Tubulin III and Nestin, when there is Rosmarinic acid, may indicate a greater induction of neuronal differentiation in Wharton’s jelly stem cells. The results show that Rosmarinic acid induces the neuronal differentiation of Wharton’s jelly stem cells.
Keywords
Cell differentiation
Wharton's jelly cells
Rosmarinic acid
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Journal of Isfahan Medical School
Volume 37, Issue 519 - Serial Number 519
January and February 2019
Pages 242-248

  • Receive Date 30 December 2018
  • Accept Date 06 April 2022