Evaluating the Level of Malondialdehyde (MDA) in Sialic Acid-Treated Human Astroglia

Document Type : Original Article (s)

Authors

1 MSc Student, Department of Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

2 PhD Student, Department of Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

3 Assistant Professor, Department of Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

4 Associate Professor, Department of Biochemistry, School of Medicine AND Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Abstract

Background: Sialic acid (SA) is presented in all cells membrane of vertebrates, and its level in the human brain is much higher than other body tissues. Studies have shown that, in addition to oxidative stress, increasing the amount of SA can also lead to the development of neurological diseases including Alzheimer's disease. Therefore, the present study was designed to evaluate the effect of SA on malondialdehyde (MDA) production levels, as a lipid peroxidation product, in human astroglia.Methods: The human astroglias were cultured in Dulbecco's Modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS), and cells were treated with different doses of SA. MDA was measured using thiobarbituric acid (TBA) protocol, and the results were analyzed using SPSS software.Findings: The production of MDA in treated cells with 200, 500, and 1000 μg/ml of SA significantly increased compared to the control group. It also significantly increased when the cells were treated with 200 μg/ml of SA at 12, 16, and 24 hours incubation.Conclusion: Many studies have been conducted on neurological disorders; however their mechanism of occurrence has not yet been fully elucidated. With regard to the role of SA in inflammation, our results suggest that SA can cause pathological conditions and oxidative stress followed by MDA elevation; which is effective in the development of neurological disorders like Alzheimer's. The role of SA and its effects need further studies.

Keywords

References

  1. Wang B, Brand-Miller J. The role and potential of sialic acid in human nutrition. Eur J Clin Nutr 2003; 57(11): 1351-69.
  2. Schnaar RL, Gerardy-Schahn R, Hildebrandt H. Sialic acids in the brain: Gangliosides and polysialic acid in nervous system development, stability, disease, and regeneration. Physiol Rev 2014; 94(2): 461-518.
  3. Rahmann H. Brain gangliosides and memory formation. Behav Brain Res 1995; 66(1): 105-16.
  4. Guzel M, Kontas Askar T, Kay G, Atakisi E, Avci G. Serum sialic acids, total antioxidant capacity, and adenosine deaminase activity in cattle with theileriosis and anaplasmosis. Bull Vet Inst Pulawy 2008; 52(2): 227-30.
  5. Citil M, Gunes V, Karapehlivan M, Atalan G, Marasli S. Evaluation of serum sialic acid as an inflammation marker in cattle with traumatic reticulo peritonitis. Rev Med Vet 2004; 155(7): 389-92.
  6. Yapar K, Kart A, Karapehlivan M, Citil M. Dose-dependent effects of L-carnitine on blood sialic acid, MDA and GSH concentrations in BALB/c mice. Acta Vet 2007; 57(4): 321-7.
  7. Davignon J, Jacob RF, Mason RP. The antioxidant effects of statins. Coron Artery Dis 2004; 15(5): 251-8.
  8. Martin MG, Perga S, Trovo L, Rasola A, Holm P, Rantamaki T, et al. Cholesterol loss enhances TrkB signaling in hippocampal neurons aging in vitro. Mol Biol Cell 2008; 19(5): 2101-12.
  9. Nazeri Z, Azizidoost S, Cheraghzadeh M, Sepiani B, Kheirollah A. The effect of 24-hydroxy cholesterol on production of reactive oxygen species (ROS) in cultured astrocytes treated by beta amyloid. J Isfahan Med Sch 2018; 36(482): 607-13. [In Persian].
  10. Rosen P, Du X, Tschope D. Role of oxygen derived radicals for vascular dysfunction in the diabetic heart: prevention by alpha-tocopherol? Mol Cell Biochem 1998; 188(1-2): 103-11.
  11. Greilberger J, Koidl C, Greilberger M, Lamprecht M, Schroecksnadel K, Leblhuber F, et al. Malondialdehyde, carbonyl proteins and albumin-disulphide as useful oxidative markers in mild cognitive impairment and Alzheimer's disease. Free Radic Res 2008; 42(7): 633-8.
  12. Perry G, Wang X, Smith M, Zhu X. Mitochondrial abnormalities in alzheimer disease. Microscopy and Microanalysis Microsc Microanal 2011; 17(S2): 200-1.
  13. Nedeljkovic ZS, Gokce N, Loscalzo J. Mechanisms of oxidative stress and vascular dysfunction. Postgrad Med J 2003; 79(930): 195-9.
  14. Engin KN, Yemisci B, Yigit U, Agachan A, Coskun C. Variability of serum oxidative stress biomarkers relative to biochemical data and clinical parameters of glaucoma patients. Mol Vis 2010; 16: 1260-71.
  15. Strzyzewski KW, Piorunska-Stolzmann M, Majewski W, Kasprzak M, Strzyzewski W. Effect of surgical treatment on lipid peroxidation parameters and antioxidant status in the serum of patients with peripheral arterial disease. Dis Markers 2013; 35(6): 647-52.
  16. Tug T, Karatas F, Terzi SM. Antioxidant vitamins (A, C and E) and malondialdehyde levels in acute exacerbation and stable periods of patients with chronic obstructive pulmonary disease. Clin Invest Med 2004; 27(3): 123-8.
  17. Ito J, Nagayasu Y, Lu R, Kheirollah A, Hayashi M, Yokoyama S. Astrocytes produce and secrete FGF-1, which promotes the production of apoE-HDL in a manner of autocrine action. J Lipid Res 2005; 46(4): 679-86.
  18. Cheraghzadeh M, Azizidoost S, Nazeri Z, Saremi S, Galehdari H, Kheirollah A. The effect of sialic acid on viability and growth of mice astrocytes and human astroglia cells. J Isfahan Med Sch 2018; 36(472): 264-9. [In Persian].
  19. Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol 1978; 52: 302-10.
  20. Varki A, Gagneux P. Multifarious roles of sialic acids in immunity. Ann N Y Acad Sci 2012; 1253: 16-36.
  21. Sawada M. Neuroprotective and toxic changes in microglia in neurodegenerative disease. Parkinsonism Relat Disord 2009; 15(Suppl 1): S39-S41.
  22. Schauer R. Sialic acids as regulators of molecular and cellular interactions. Curr Opin Struct Biol 2009; 19(5): 507-14.
  23. Palmer AM, Burns MA. Selective increase in lipid peroxidation in the inferior temporal cortex in Alzheimer's disease. Brain Res 1994; 645(1-2): 338-42.
  24. Babri S, Mohaddes G, Feizi I, Mohammadnia A, Niapour A, Alihemmati A, et al. Effect of troxerutin on synaptic plasticity of hippocampal dentate gyrus neurons in a beta-amyloid model of Alzheimers disease: an electrophysiological study. Eur J Pharmacol 2014; 732: 19-25.
  25. Ekdahl CT, Kokaia Z, Lindvall O. Brain inflammation and adult neurogenesis: the dual role of microglia. Neuroscience 2009; 158(3): 1021-9.
  26. Sohn H, Kim YS, Kim HT, Kim CH, Cho EW, Kang HY, et al. Ganglioside GM3 is involved in neuronal cell death. FASEB J 2006; 20(8): 1248-50.
  27. Yanagisawa K, Odaka A, Suzuki N, Ihara Y. GM1 ganglioside-bound amyloid beta-protein (A beta): a possible form of preamyloid in Alzheimer's disease. Nat Med 1995; 1(10): 1062-6.
Journal of Isfahan Medical School
Volume 37, Issue 520 - Serial Number 520
January and February 2019
Pages 263-269

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History

  • Receive Date: 11 April 2019
  • Revise Date: 28 April 2024
  • Accept Date: 06 April 2022

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