Journal of Isfahan Medical School

Journal of Isfahan Medical School

In-vitro Toxicity Assessment of Polydopamine-Coated and Uncoated Fe3O4 Nanoparticles in Cell Line B16-F10 (Melanoma Cell)

Document Type : Original Article (s)

Authors
Fahimeh Hossein-Beigi 1
Soheil Fatahian 2
Daryoush Shahbazi-Gahrouei 3
1 MSc Student, Department of Cellular and Molecular Biology (Biophysics), Falavarjan Branch, Islamic Azad University, Isfahan, Iran
2 Assistant Professor, Department of Cellular and Molecular Biology (Biophysics), Falavarjan Branch, Islamic Azad University, Isfahan, Iran
3 Professor, Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
10.22122/jims.v37i533.12145
Abstract
Background: In recent years, due to their small size and increased surface-to-volume ratio, the use of nanoparticles in diagnosis, drug delivery, and treatment has received much attention. The most important problem in chemotherapy is the limited access to the central part of the tumor due to its low blood flow. The aim of this study was to investigate the toxicity of polydopamine-coated and uncoated Fe3O4 nanoparticles on melanoma B16-F10 cells.Methods: Fe3O4 nanoparticles were co-precipitated and coated with dopamine. Then, the effect of cytotoxicity of the coated and uncoated nanoparticles on the B16-F10 cells was studied using MTT assay.Findings: Uncoated Fe3O4 nanoparticles were toxic to B16-F10 at the concentration of 450 μg/ml and duration of 72 hours. Coated nanoparticles had no toxicity at any concentration at the duration of 48 and 72 hours.Conclusion: The cytotoxic properties of uncoated nanoparticles were significantly higher than the coated nanoparticles. Polydopamine coating shell can reduce the toxicity of the nanoparticles, and it can be used for bonding the drugs, as well as a contrast agent in magnetic resonance imaging (MRI).
Keywords
Iron oxide
Nanoparticles
Polydopamine
Melanoma
Cytotoxicity
  1. Fatahian S, Shahbazi-Gahrouei D, Pouladian M, Yousefi MH, Amiri GR, Noori A. Biodistribution and toxicity assessment of radiolabeled and DMSA coated ferrite nanoparticles in mice. J Radioanal Nucl Chem 2012; 293(3): 915-21.
  2. Shahbazi-Gahrouei D, Moradi KP, Moradi KB, Shahbazi-Gahrouei S. Medical imaging modalities using nanoprobes for cancer diagnosis: A literature review on recent findings. J Res Med Sci 2019; 24: 38.
  3. Shahbazi-Gahrouei D, Moradi Khaniabadi P, Shahbazi-Gahrouei S, Khorasani A, Mahmoudi F. A literature review on multimodality molecular imaging nanoprobes for cancer detection. Polish Journal of Medical Physics and Engineering 2019; 25(2): 57-68.
  4. Pankhurst QA, Thanh NTK, Jones SK, Dobson J. Progress in applications of magnetic nanoparticles in biomedicine. J Phys D Appl Phys 2009; 42(22): 224001.
  5. Moradi KP, Shahbazi-Gahrouei D, Malik Shah Abdul Majid A, Suhaimi JM, Moradi KB, Shahbazi-Gahrouei S. In vitro study of SPIONs-C595 as molecular imaging probe for specific breast cancer (MCF-7) cells detection. Iran Biomed J 2017; 21(6): 360-8.
  6. Majewski AP, Schallon A, Jerome V, Freitag R, Muller AH, Schmalz H. Dual-responsive magnetic core-shell nanoparticles for nonviral gene delivery and cell separation. Biomacromolecules 2012; 13(3): 857-66.
  7. Gu X, Zhang Y, Sun H, Song X, Fu C, Dong P. Mussel-inspired polydopamine coated iron oxide nanoparticles for biomedical application. J Nanomater 2015; 2015: 154592.
  8. Liao N, Wu M, Pan F, Lin J, Li Z, Zhang D, et al. Poly (dopamine) coated superparamagnetic iron oxide nanocluster for noninvasive labeling, tracking, and targeted delivery of adipose tissue-derived stem cells. Sci Rep 2016; 6: 18746.
  9. Raeisi F, Raeisi E, Shahbazi-Gahrouei D, Heidarian E, Amiri M, Gholami M. Cytotoxicity effect of pineapple extract on breast cancer cells (4T1). J Isfahan Med Sch 2016; 34(394): 946-51. [In Persian].
  10. Fatahian S, Shahbazi-Gahrouei D, Pouladian M, Yousefi MH, Amiri G, Shahi Z, et al. Preparation and magnetic properties investigation of Fe3O4 nanoparticles 99mTc labeled and Fe3O4 nanoparticles DMSA coated. Dig J Nanomater Bios 2011; 6(3): 1161-5.
  11. Martin M, Salazar P, Villalonga R, Campuzano S, Pingarron JM, Gonzalez-Moraa JL. Preparation of core–shell Fe3O4@poly(dopamine) magnetic nanoparticles for biosensor construction. J Mater Chem B 2014; 2(6): 739-46.
  12. Butler M. Animal cell culture and technology. London, UK: Taylor and Francis; 2004.
  13. Yan W, Lien HL, Koel BE, Zhang WX. Iron nanoparticles for environmental clean-up: recent developments and future outlook. Environ Sci Process Impacts 2013; 15(1): 63-77.
  14. Mahdavi M, Ahmad MB, Haron MJ, Namvar F, Nadi B, Rahman MZ, et al. Synthesis, surface modification and characterisation of biocompatible magnetic iron oxide nanoparticles for biomedical applications. Molecules 2013; 18(7): 7533-48.
  15. Mai TT, Moon J, Song Y, Viet PQ, Phuc PV, Lee JM, et al. Ginsenoside F2 induces apoptosis accompanied by protective autophagy in breast cancer stem cells. Cancer Lett 2012; 321(2): 144-53.
  16. Manke A, Wang L, Rojanasakul Y. Mechanisms of nanoparticle-induced oxidative stress and toxicity. Biomed Res Int 2013; 2013: 942916.
  17. Ankamwar B, Lai TC, Huang JH, Liu RS, Hsiao M, Chen CH, et al. Biocompatibility of Fe(3)O(4) nanoparticles evaluated by in vitro cytotoxicity assays using normal, glia and breast cancer cells. Nanotechnology 2010; 21(7): 75102.
  18. Khan MI, Mohammad A, Patil G, Naqvi SA, Chauhan LK, Ahmad I. Induction of ROS, mitochondrial damage and autophagy in lung epithelial cancer cells by iron oxide nanoparticles. Biomaterials 2012; 33(5): 1477-88.
  19. Wang LS, Chuang MC, Ho JA. Nanotheranostics--a review of recent publications. Int J Nanomedicine 2012; 7: 4679-95.
  20. Mahmoudi M, Simchi A, Vali H, Imani M, Shokrgozar MA, Azadmanesh K, et al. Cytotoxicity and cell cycle effects of bare and poly(vinyl alcohol)-coated iron oxide nanoparticles in mouse fibroblasts. Adv Eng Mater 2009; 11(12): B243-B250.
  21. Dung TT, Danh TM, Hoa LTM, Chien DM, Duc NH. Structural and magnetic properties of starch-coated magnetite nanoparticles. J Exp Nanosci 2009; 4(3): 259-67.
  22. Ghahremani F, Shahbazi-Gahrouei D, Kefayat A, Motaghi H, Mehrgardi MA, Haghjooy Javanmard S. AS1411 aptamer conjugated gold nanoclusters as a targeted radiosensitizer for megavoltage radiation therapy of 4T1 breast cancer cells. RSC Advances 2018; 8(8): 4249-58.
Journal of Isfahan Medical School
Volume 37, Issue 533 - Serial Number 533
May and June 2019
Pages 762-767

  • Receive Date 12 August 2019
  • Accept Date 06 April 2022