The Effect of Crosin on Expression of Interleukin 6 in Human Glioblastoma Cell Line

Document Type : Original Article (s)

Authors

1 Student, Department of Genetics, School of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Professor, Department of Genetics, School of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Assistant Professor, Department of Pharmacology, School of Pharmacy AND Medicinal Plant Research Center, Ahvaz Jundishapur University of Medical sciences, Ahvaz, Iran

Abstract

Background: Crosin is one of the most important ingredients of saffron (the Iridaceae family) that has been emphasized in Iranian traditional medicine for its anti-inflammatory and anti-tumor effects. Glioblastoma is one of the most malignant cancers resistant to radiation and chemotherapy in adults. The goal of the present study was to investigate the effect crosin on expression of interleukin 6 (IL-6) in human glioblastoma cell line.Methods: Human glioblastoma cell line (1321N1) was purchased from Pasteur Institute of Iran. Cell was grown in Dulbico’s modified essential media (DMEM) supplemented whit 10% fetal bovine serum (FBS). The half-maximal inhibitory concentration (IC50) value of crosin was obtained using MTT assay. Glioblastoma cell line was treated by different concentration of crosin (5, 10, 15 mM/ml) for 24 hours. Then, total RNA was isolated from cells and used for cDNA synthesis. Expression level of IL-6 was assessed using real-time polymerase chain reaction (real-time PCR).Findings: The IC50 value of crosin against 1321N1 cells after 24 hours were determined as 53.3 mMol/ml. Moreover, the expression of IL-6 gene was downregulated by increasing of crosin concentration compare to the control group (P < 0.050).Conclusion: It was observed that crosin inhibited production of IL-6 in glioblastoma cells. In conclusion, the result suggested crosin as a potential therapeutic candidate for neuroinflammation in glioblastoma cancer.

Keywords

References

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Journal of Isfahan Medical School
Volume 37, Issue 537 - Serial Number 537
July and August 2019
Pages 903-909

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History

  • Receive Date: 26 August 2019
  • Revise Date: 03 May 2024
  • Accept Date: 06 April 2022

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