Journal of Isfahan Medical School

Journal of Isfahan Medical School

The Effect of Eight Weeks of High-Interval Training on Activin Receptor Type II in Heart and Plasma Levels of Myostatin in Male Rats

Document Type : Original Article (s)

Authors
Maijd Beigi 1
Saeed Keshavarz 2
Jamshid Banaei 3
Hamid Zahedi 3
1 PhD Student, Department of Sport Sciences, Najafabad Branch, Islamic Azad University, Najafabad, Iran
2 Assistant Professor, Department of Sport Sciences, Najafabad Branch, Islamic Azad University, Najafabad, Iran
3 Assistant Professor, Sport Medicine Research Center, Najafabad Branch, Islamic Azad University, Najafabad, Iran
10.22122/jims.v38i561.12718
Abstract
Background: Exercise trainings have beneficial effects on myocardium by changing the concentration of growth factors and their receptors. The aim of this study was to determine the effect of eight weeks of high-intensity interval training on activin receptor type II in heart and plasma levels of myostatin in male rats.Methods: After the familiarization period, twenty male rats with the limitation of three months of age were randomly divided to control (n = 10) and high-intensity interval training (n=10) groups. High-intensity interval training was continued for eight weeks, five sessions in each week and each session lasted 80 minutes with 85-90 percent intensity of rats’ power with 20-degree ramp. Activin receptor type II in the myocardial left ventricle was measured using immunohistochemistry method, and myostatin was measured by the enzyme-linked immunosorbent assay (ELISA) method. The data were analyzed using independent-samples t test at the statistically significant level of P < 0.050.Findings: In between groups comparison, there was a significant difference between the high-intensity interval training and control groups in myostatin variable (P = 0.001). There was no significant difference between the groups in myocardium activin receptor type II (P = 0.820).Conclusion: It cautiously seems that high-intensity interval training have desirable effects on reducing myostatin, but it has no effect on heart activin receptor type II.
Keywords
High-intensity Intermittent Exercise
Activin receptors
Myostatin
Male
Rats
  1. Fathi M, Abroun S. The effect of 14 weeks of endurance training on miR-499 expression of left ventricle in wistar male rat. Sport Physiology 2016; 7(28): 59-72. [In Persian].
  2. Kordi M, Nekouei A, Shafiee, Hadidi V. The effect of eight weeks high intensity aerobic continuous and interval training on gene expression of vascular endothelial growth factor in soleus muscle of healthy male rats. J Arak Univ Med Sci 2015; 18(8): 53-62. [In Persian].
  3. Fathi M, Gharakhanlou R. The effect of endurance Activity on left ventricle Hand2 gene expression in wistar male rat. Sport Psychology 2015; 7(25): 57-68. [In Persian].
  4. Holloway TM, Bloemberg D, da Silva ML, Simpson JA, Quadrilatero J, Spriet LL. High intensity interval and endurance training have opposing effects on markers of heart failure and cardiac remodeling in hypertensive rats. PLoS One 2015; 10(3): e0121138.
  5. Tang YD, Katz SD. Anemia in chronic heart failure: Prevalence, etiology, clinical correlates, and treatment options. Circulation 2006; 113(20): 2454-61.
  6. Elkina Y, von Haehling S, Anker SD, Springer J. The role of myostatin in muscle wasting: an overview. J Cachexia Sarcopenia Muscle 2011; 2(3): 143-51.
  7. Lee SJ, McPherron AC. Regulation of myostatin activity and muscle growth. Proc Natl Acad Sci USA 2001; 98(16): 9306-11.
  8. Ryan AS, Ivey FM, Prior S, Li G, Hafer-Macko C. Skeletal muscle hypertrophy and muscle myostatin reduction after resistive training in stroke survivors. Stroke 2011; 42(2): 416-20.
  9. Butcher JT, Ali MI, Ma MW, McCarthy CG, Islam BN, Fox LG, et al. Effect of myostatin deletion on cardiac and microvascular function. Physiol Rep 2017; 5(23): e13525.
  10. Bueno PG, Bassi D, Contrera DG, Carnielli HM, Silva RN, Nonaka KO, et al. Post-exercise changes in myostatin and actRIIB expression in obese insulin-resistant rats. Mol Cell Endocrinol 2011; 339(1): 159-64.
  11. Morvan F, Rondeau JM, Zou C, Minetti G, Scheufler C, Scharenberg M, et al. Blockade of activin type II receptors with a dual anti-ActRIIA/IIB antibody is critical to promote maximal skeletal muscle hypertrophy. Proc Natl Acad Sci USA 2017; 114(47): 12448-53.
  12. Bayarsaikhan O, Kawai N, Mori H, Kinouchi N, Nikawa T, Tanaka E. Co-administration of myostatin-targeting siRNA and ActRIIB-Fc fusion protein increases masseter muscle mass and fiber size. J Nutr Sci Vitaminol (Tokyo) 2017; 63(4): 244-8.
  13. Bashiri J, NourAzar A, Purrazi H. Effect of three months aerobic training on Wnt-signaling pathway in skeletal muscle of male rats. Razi J Med Sci 2017; 24(160): 7-16. [In Persian].
  14. Shirvani H, Aslani J. The effects of high-intensity interval training vs. moderate-intensity continuous training on serum irisin and expression of skeletal muscle PGC-1a gene in male rats. Tehran Univ Med J 2017; 75(7): 513-20. [In Persian].
  15. Chavanelle V, Boisseau N, Otero YF, Combaret L, Dardevet D, Montaurier C, et al. Effects of high-intensity interval training and moderate-intensity continuous training on glycaemic control and skeletal muscle mitochondrial function in db/db mice. Sci Rep 2017; 7(1): 204.
  16. Negaresh R, Ranjbar R, Habibi A, Mokhtarzade M, Fokin A, Gharibvand MM. The effect of resistance training on quadriceps muscle volume and some growth factors in elderly and young men. Adv Gerontol 2017; 30(6): 880-7.
  17. Hittel DS, Axelson M, Sarna N, Shearer J, Huffman KM, Kraus WE. Myostatin decreases with aerobic exercise and associates with insulin resistance. Med Sci Sports Exerc 2010; 42(11): 2023-9.
  18. Paoli A, Pacelli QF, Neri M, Toniolo L, Cancellara P, Canato M, et al. Protein supplementation increases postexercise plasma myostatin concentration after 8 weeks of resistance training in young physically active subjects. J Med Food 2015; 18(1): 137-43.
  19. Santos AR, Neves MT, Gualano B, Laurentino GC, Lancha AH, Ugrinowitsch C, et al. Blood flow restricted resistance training attenuates myostatin gene expression in a patient with inclusion body myositis. Biol Sport 2014; 31(2): 121-4.
  20. Biglari S, Gaeini AA, Kordi MR, Ghardashi Afousi A. The effect of 8 weeks high-intensity interval training on myostatin and follistatin gene expression in gastrocnemius muscle of the rats. J Arak Uni Med Sci 2018; 21(1): 1-10. [In Persian].
  21. Karbalaeifar S, Gaeini A, Kordi M, Nuri R, Ghorbani P. Effect of 6 weeks high intensity interval training on selected factors of cardiac angiogenesis in rats with myocardial infarction. Sport Physiology, 2019; 11(42): 17-30. [In Persian].
  22. Hulmi JJ, Ahtiainen JP, Kaasalainen T, Pollanen E, Hakkinen K, Alen M, et al. Postexercise myostatin and activin IIb mRNA levels: Effects of strength training. Med Sci Sports Exerc 2007; 39(2): 289-97.
  23. Egerman MA, Cadena SM, Gilbert JA, Meyer A, Nelson HN, Swalley SE, et al. GDF11 Increases with age and inhibits skeletal muscle regeneration. Cell Metab 2015; 22(1): 164-74.
  24. Poggioli T, Vujic A, Yang P, Macias-Trevino C, Uygur A, Loffredo FS, et al. Circulating growth differentiation factor 11/8 levels decline with age. Circ Res 2016; 118(1): 29-37.
  25. Bondulich MK, Jolinon N, Osborne GF, Smith EJ, Rattray I, Neueder A, et al. Myostatin inhibition prevents skeletal muscle pathophysiology in Huntington's disease mice. Sci Rep 2017; 7(1): 14275.
  26. Wilkes JJ, Lloyd DJ, Gekakis N. Loss-of-function mutation in myostatin reduces tumor necrosis factor alpha production and protects liver against obesity-induced insulin resistance. Diabetes 2009; 58(5): 1133-43.
  27. Lipina C, Kendall H, McPherron AC, Taylor PM, Hundal HS. Mechanisms involved in the enhancement of mammalian target of rapamycin signalling and hypertrophy in skeletal muscle of myostatin-deficient mice. FEBS Lett 2010; 584(11): 2403-8.
  28. Masoudian B, Azamian Jazi A, Faramarzi M, Talebi A. The effect of an 8-week resistance training on ActRIIß in fast- and slow-twitch skeletal muscles and plasma levels of GDF8, GDF11 and GASP-1 in old male rats. Razi J Med Sci 2019; 25(12):104-15. [In Persian].
  29. Masoudian B, Azamian-Jazi A, Faramarzi M, Talebi A. The effect of eight weeks of resistance training on activin receptor type II-B and plasma levels of growth differentiation factor 11 and 8 in cardiac physiological hypertrophy in male wistar rats. J Isfahan Med Sch 2019; 36(502): 1308-13. [In Persian].
  30. Yang J, Sun L, Fan X, Yin B, Kang Y, Tang L, et al. Effect of exercise on bone in poorly controlled type 1 diabetes mediated by the ActRIIB/Smad signaling pathway. Exp Ther Med 2018; 16(4): 3686-93.
  31. Lach-Trifilieff E, Minetti GC, Sheppard K, Ibebunjo C, Feige JN, Hartmann S, et al. An antibody blocking activin type II receptors induces strong skeletal muscle hypertrophy and protects from atrophy. Mol Cell Biol 2014; 34(4): 606-18.
  32. Chiu CS, Peekhaus N, Weber H, Adamski S, Murray EM, Zhang HZ, et al. Increased muscle force production and bone mineral density in ActRIIB-Fc-treated mature rodents. J Gerontol A Biol Sci Med Sci 2013; 68(10): 1181-92.
Journal of Isfahan Medical School
Volume 38, Issue 561 - Serial Number 561
January and February 2020
Pages 8-13

  • Receive Date 08 February 2020
  • Accept Date 06 April 2022