Journal of Isfahan Medical School

Journal of Isfahan Medical School

Evaluation of Cytotoxic Effects of the Combination of Metformin with Docetaxel and 5-Fluorouracil on the Gastric Cancer Cells

Document Type : Original Article (s)

Authors
Maryam Fatehi-Aghdam 1
Nowruz Najafzadeh 2
Mohammad Ghasem Golmohammadi 2
1 MSc Student, Department of Anatomical Sciences and Pathology, School of Medicine and Paramedicine, Ardabil University of Medical Sciences, Ardabil, Iran
2 Associate Professor, Research Laboratory for Embryology and Stem Cells AND Department of Anatomical Sciences and Pathology, School of Medicine and Paramedicine, Ardabil University of Medical Sciences, Ardabil, Iran
10.22122/jims.v38i567.12384
Abstract
Background: In the present study, the cytotoxic effects of the combination of metformin with docetaxel and 5- fluorouracil were studied on AGS gastric cancer cell line.Methods: In this experimental study, the cells were exposed to different concentrations of metformin (1-80 mM), docetaxel (0.6-22.5 ng/ml), 5-fluorouracil (0.045-12 μg/ml), and combination of them. The cell proliferation was evaluated using MTT assay, and the cell apoptosis was evaluated by acridine orange/ethidium bromide assay.Findings: Metformin, docetaxel, and 5-fluorouracil inhibited cell viability of AGS gastric cancer cells in a concentration- and time-dependent manner. The half-maximal inhibitory concentration (IC50) values were decreased after treatment with metformin/docetaxel and metformin/5-fluorouracil (P < 0.050). Moreover, the combination treatments significantly increased apoptosis of gastric cancer cells (P < 0.050).Conclusion: Our results showed that the combination of metformin with docetaxel and 5-fluorouracil could be effective in the treatment of patients with gastric cancer.
Keywords
Metformin
Docetaxel
5-Fluorouracil
Gastric cancer
Apoptosis
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Journal of Isfahan Medical School
Volume 38, Issue 567 - Serial Number 567
January and February 2020
Pages 139-146

  • Receive Date 16 October 2019
  • Accept Date 06 April 2022