Journal of Isfahan Medical School

Journal of Isfahan Medical School

The Effects of Aerobic Training in Polluted Air and Vitamin C Supplementation on Serum Levels of Endothelin-1 and Nitric Oxide in Wistar Rats

Document Type : Original Article (s)

Authors
Amin Eatemady-Boroujeni 1
Mehdi Kargarfard 2
Hojatallah Alaei 3
1 PhD Candidate, Department of Exercise Physiology, School of Sport Sciences, University of Isfahan, Isfahan, Iran
2 Professor, Department of Exercise Physiology, School of Sport Sciences, University of Isfahan, Isfahan, Iran
3 Professor, Department of Medical Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
10.22122/jims.v38i576.12581
Abstract
Background: There is little information on the effects of exercise in polluted air and vitamin C intake on plasma factors. The aim of this study was to investigate the effect of twelve weeks of aerobic training in polluted air and vitamin C supplementation on serum levels of endothelin-1 and nitric oxide in Wistar rats.Methods: 50 male Wistar rats were randomly divided into five groups of training, training-supplementation, training-pollution, training-pollution-supplementation, and control. Training protocol consisted of aerobic training for 40 minutes with intensity of 65%-80% of maximal speed for 12 weeks and 5 sessions per week. The amount of vitamin C consumed in each rat was 20 mg/kg/day used orally. Plasma levels of endothelin-1 and nitric oxide were measured at the end of the study. Data were analyzed using one-way analysis of variance.Findings: Endothelin-1 levels significantly increased in polluted air groups; vitamin C was able to decrease endothelin-1 levels (P < 0.05). The level of nitric oxide increased significantly in training in polluted air groups. These results were significantly different from the training-pollution-supplementation group (P < 0.05).Conclusion: According to the results of this study, vitamin C intake is effective in decreasing plasma levels of endothelin-1 during exposure to air pollution. The effect of exercise and polluted air on nitric oxide was significant, and vitamin C alone reduced nitric oxide in training in polluted air group.
Keywords
Endothelin-1
Nitric oxide
Air Pollution
Vitamin C
Aerobic training
  1. Reilly T, Waterhouse JM, Waterhouse J, Budgett R. Sport, exercise and environmental physiology. London, UK: Elsevier Churchill Livingstone; 2005.
  2. Gunga HC. Human physiology in extreme environments. Cambridge, MA: Academic Press; 2014.
  3. Kargarfard M, Shariat A, Shaw BS, Shaw I, Lam ET, Kheiri A, et al. Effects of polluted air on cardiovascular and hematological parameters after progressive maximal aerobic exercise. Lung 2015; 193(2): 275-81.
  4. Nadadur SS, Hollingsworth JW. Air pollution and health effects. London, UK: Springer London; 2015.
  5. Brook RD. Is air pollution a cause of cardiovascular disease? Updated review and controversies. Rev Environ Health 2007; 22(2): 115-37.
  6. Calderon-Garciduenas L, Vincent R, Mora-Tiscareno A, Franco-Lira M, Henriquez-Roldan C, Barragan-Mejia G, et al. Elevated plasma endothelin-1 and pulmonary arterial pressure in children exposed to air pollution. Environ Health Perspect 2007; 115(8): 1248-53.
  7. Hall JE. Guyton and Hall textbook of medical physiology. Saint Louis, MO: Elsevier Health Sciences; 2015.
  8. Higashi Y, Yoshizumi M. New methods to evaluate endothelial function: Method for assessing endothelial function in humans using a strain-gauge plethysmography: Nitric oxide-dependent and -independent vasodilation. J Pharmacol Sci 2003; 93(4): 399-404.
  9. Deanfield JE, Halcox JP, Rabelink TJ. Endothelial function and dysfunction: Testing and clinical relevance. Circulation 2007; 115(10): 1285-95.
  10. Briet M, Collin C, Laurent S, Tan A, Azizi M, Agharazii M, et al. Endothelial function and chronic exposure to air pollution in normal male subjects. Hypertension 2007; 50(5): 970-6.
  11. Barton M, Yanagisawa M. Endothelin: 20 years from discovery to therapy. Can J Physiol Pharmacol 2008; 86(8): 485-98.
  12. Farrell PA, Joyner MJ, Caiozzo VJ, American College of Sports Medicine. ACSM's advanced exercise physiology. Philadelphia, PA: Lippincott Williams and Wilkins; 2012.
  13. Thomson E, Goegan P, Kumarathasan P, Vincent R. Air pollutants increase gene expression of the vasoconstrictor endothelin-1 in the lungs. Biochim Biophys Acta 2004; 1689(1): 75-82.
  14. Moser MA, Chun OK. Vitamin C and heart health: A review based on findings from epidemiologic studies. Int J Mol Sci 2016; 17(8): 1328.
  15. Leandro CG, Levada AC, Hirabara SM, Manhaes-de-Castro R, De-Castro CB, Curi R, et al. A program of moderate physical training for Wistar rats based on maximal oxygen consumption. J Strength Cond Res 2007; 21(3): 751-6.
  16. Coskun S, Gonul B, Guzel NA, Balabanli B. The effects of vitamin C supplementation on oxidative stress and antioxidant content in the brains of chronically exercised rats. Mol Cell Biochem 2005; 280(1): 135-8.
  17. American Physiological Society. Resource Book for the Design of Animal Exercise Protocols. Rockville, MD: American Physiological Society; 2006.
  18. Isfahan Department of environment. Air quality of Isfahan [Online]. [cited 2019]; Available from: URL: http://www.isfahan-doe.ir/Index.aspx?page_=form&lang=1&sub=0&tempname=default&PageID=5
  19. Archer CR, Robinson EL, Drawnel FM, Roderick HL. Endothelin-1 promotes hypertrophic remodelling of cardiac myocytes by activating sustained signalling and transcription downstream of endothelin type A receptors. Cell Signal 2017; 36: 240-54.
  20. Tammen H. Specimen Collection and Handling. In: Vlahou A, editor. Clinical proteomics: Methods and Protocols. Totowa, NJ: Humana Press; 2008. p. 35-42.
  21. Shapiro SS, Wilk MB. An analysis of variance test for normality (Complete samples). Biometrika 1965; 52(3/4): 591-611.
  22. Kaehler J, Sill B, Koester R, Mittmann C, Orzechowski HD, Muenzel T, et al. Endothelin-1 mRNA and protein in vascular wall cells is increased by reactive oxygen species. Clin Sci (Lond) 2002; 103 Suppl 48: 176S-8S.
  23. Calderon-Garciduenas L, Gambling TM, Acuna H, Garcia R, Osnaya N, Monroy S, et al. Canines as sentinel species for assessing chronic exposures to air pollutants: part 2. Cardiac pathology. Toxicol Sci 2001; 61(2): 356-67.
  24. Bohm F, Settergren M, Pernow J. Vitamin C blocks vascular dysfunction and release of interleukin-6 induced by endothelin-1 in humans in vivo. Atherosclerosis 2007; 190(2): 408-15.
  25. Powers SK, Howley ET. Exercise physiology: Theory and Application to fitness and performance. New York, NY: McGraw-Hill Education; 2015.
  26. Fleming I. Molecular mechanisms underlying the activation of eNOS. Pflugers Arch 2010; 459(6): 793-806.
  27. Thomson EM, Kumarathasan P, Calderon-Garciduenas L, Vincent R. Air pollution alters brain and pituitary endothelin-1 and inducible nitric oxide synthase gene expression. Environ Res 2007; 105(2): 224-33.
  28. Ignarro LJ. Nitric Oxide: Biology and Pathobiology. San Diego, CA: Academic Press; 2009.
  29. Kasimay O, Ergen N, Bilsel S, Kacar O, Deyneli O, Gogas D, et al. Diet-supported aerobic exercise reduces blood endothelin-1 and nitric oxide levels in individuals with impaired glucose tolerance. J Clin Lipidol 2010; 4(5): 427-34.
  30. Pereira AC, Araujo AV, Paulo M, Andrade FA, Silva BR, Vercesi JA, et al. Hypotensive effect and vascular relaxation in different arteries induced by the nitric oxide donor RuBPY. Nitric Oxide 2017; 62: 11-6.
Journal of Isfahan Medical School
Volume 38, Issue 576 - Serial Number 576
March and April 2020
Pages 339-346

  • Receive Date 02 January 2020
  • Accept Date 06 April 2022