Journal of Isfahan Medical School

Journal of Isfahan Medical School

The Effects of Prednisolone and Mometasone on Wound Healing Caused by Leishmania Major in Balb/c Mice

Document Type : Original Article (s)

Authors
Ghazaleh Mahabadi 1
Sedigheh Saberi 2
Seyyed Hossein Hejazi-Dehaghani 3
Zahra Ghayour najafabadi 2
1 MSc Student, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 Assistant Professor, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3 Professor, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
10.22122/jims.v38i580.12405
Abstract
Background: An effective vaccine against leishmaniasis is not available, and chemotherapy is the only effective way to treat all forms of the disease. However, current therapy is toxic and expensive, and the resistance has emerged as a serious problem, which has compelled the search for new antileishmanial agents such as glucocorticoids. The aim of this study was to investigate the effects of prednisolone and mometasone on the improvement of cutaneous leishmaniasis in BALB/c mice.Methods: In this experimental study, 60 female Balb/c mice were used. The promastigote form obtained from the culture medium was injected into the mice's tail base. After the creation of wound, the mice were divided into 6 treatment groups of prednisolone, prednisolone and amphotericin B, mometasone, mometasone and amphotericin B, positive control (amphotericin B), and negative control (phosphate buffered saline or PBS). The duration of treatment was 28 days. The lesion diameter was measured at the end of each week.Findings: After the end of the treatment period, significant reduction was observed in wound size and parasite load in the spleen in the two groups treated with prednisolone and prednisolone combined with amphotericin B (P < 0.050).Conclusion: According to the findings of the study, it seems that glucocorticoids likely prevent chronic disease, and accelerate wound healing by regulating immune pathways.
Keywords
Prednisolone
Mometasone
Leishmaniasis, Cutaneous
  1. Akhoundi M, Downing T, Votypka J, Kuhls K, Lukes J, Cannet A, et al. Leishmania infections: Molecular targets and diagnosis. Mol Aspects Med 2017; 57: 1-29.
  2. Talari SA, Sadr F. Treatment of Cutaneous leishmaniasis: Effectiveness, and adverse effects of the drugs. Feyz 2005; 9(1): 85-94. [In Persian].
  3. Tabatabaee F, Ghaffarifar F, Dalimi A, Ebrahimian H. Effects of new herbal formulation of Euphorbia mysinites, Alkanna tinctoria and Peganum harmala combination against experimental leishmaniasis in Balb/C mice. Daneshvar Med 2005; 12(57): 37-46. [In Persian].
  4. Sachdeva H. Life cycle of Leishmania donovani; causative agent of visceral leishmaniasis: A review. International Journal of Scientific Research in Science, Engineering and Technology 2016; 2(1): 84-5.
  5. Chapman LAC, Morgan ALK, Adams ER, Bern C, Medley GF, Hollingsworth TD. Age trends in asymptomatic and symptomatic Leishmania donovani infection in the Indian subcontinent: A review and analysis of data from diagnostic and epidemiological studies. PLoS Negl Trop Dis 2018; 12(12): e0006803.
  6. Scott P, Novais FO. Cutaneous leishmaniasis: Immune responses in protection and pathogenesis. Nat Rev Immunol 2016; 16(9): 581-92.
  7. Khayeka-Wandabwa C, Zhou G, Magak NG, Choge JK, Kemei WK, Makwali JA, et al. Combined chemotherapy manifest less severe immunopathology effects in helminth-protozoa comorbidity. Exp Parasitol 2019; 204: 107728.
  8. Berrebi D, Bruscoli S, Cohen N, Foussat A, Migliorati G, Bouchet-Delbos L, et al. Synthesis of glucocorticoid-induced leucine zipper (GILZ) by macrophages: an anti-inflammatory and immunosuppressive mechanism shared by glucocorticoids and IL-10. Blood 2003; 101(2): 729-38.
  9. Boussoffara T, Boubaker MS, Ben AM, Mokni M, Guizani I, Ben SA, et al. Histological and immunological differences between zoonotic cutaneous leishmaniasis due to Leishmania major and sporadic cutaneous leishmaniasis due to Leishmania infantum. Parasite 2019; 26: 9.
  10. Reimao JQ, Trinconi CT, Yokoyama-Yasunaka JK, Miguel DC, Kalil SP, Uliana SR. Parasite burden in Leishmania (Leishmania) amazonensis-infected mice: Validation of luciferase as a quantitative tool. J Microbiol Methods 2013; 93(2): 95-101.
  11. Yorek MS, Poudel B, Mazgaeen L, Pope RM, Wilson ME, Gurung P. Leishmania major degrades murine CX. PLoS Negl Trop Dis 2019; 13(7): e0007533.
  12. Mukherjee S, Xu W, Hsu FF, Patel J, Huang J, Zhang K. Sterol methyltransferase is required for optimal mitochondrial function and virulence in Leishmania major. Mol Microbiol 2019; 111(1): 65-81.
  13. Diefenbach A, Schindler H, Rollinghoff M, Yokoyama WM, Bogdan C. Requirement for type 2 NO synthase for IL-12 signaling in innate immunity. Science 1999; 284(5416): 951-5.
  14. Holaday BJ, Sadick MD, Wang ZE, Reiner SL, Heinzel FP, Parslow TG, et al. Reconstitution of Leishmania immunity in severe combined immunodeficient mice using Th1- and Th2-like cell lines. J Immunol 1991; 147(5): 1653-8.
  15. Scott P, Natovitz P, Coffman RL, Pearce E, Sher A. Immunoregulation of cutaneous leishmaniasis. T cell lines that transfer protective immunity or exacerbation belong to different T helper subsets and respond to distinct parasite antigens. J Exp Med 1988; 168(5): 1675-84.
  16. Moll H, Scollay R, Mitchell GF. Resistance to cutaneous leishmaniasis in nude mice injected with L3T4+ T cells but not with Ly-2+ T cells. Immunol Cell Biol 1988; 66 (Pt 1): 57-63.
  17. Kumari S. Relevance of natural killer T cell NKT in visceral leishmaniasis. [thesis]. Calcutta, India: University of Calcutta; 2017.
  18. Mougneau E, Bihl F, Glaichenhaus N. Cell biology and immunology of Leishmania. Immunol Rev 2011; 240(1): 286-96.
  19. Sacks D, Noben-Trauth N. The immunology of susceptibility and resistance to Leishmania major in mice. Nat Rev Immunol 2002; 2(11): 845-58.
  20. Osero BO, Mosigisi A, Ogeto TK, Mugambi R, Ingonga J, Karanja RM, Gicheru M, Anjili C. Effects of glucocorticoids in Leishmania major infection. International Journal of Fauna and Biological Studies 2015; 2(3): 16-22.
  21. Nyamao R, Osiemo Lagat Z, Christopher K, Jumba B, Waihenya R. Efficacy of glucocorticoids in controlling leishmania major infecting Balb/c mice. J Infect Dis Immun 2014; 6(2): 10-8.
  22. Hosseini Renani E, Soleimanifard S, Hejazi SH, Ghayour Najafabadi Z. An investigation of the effects of the glucocorticoid on Leishmania major Amastigotes. Avicenna J Clin Microbiol Infect 2019; 6(4): 127-32.
Journal of Isfahan Medical School
Volume 38, Issue 580 - Serial Number 580
May and June 2020
Pages 415-421

  • Receive Date 24 October 2019
  • Accept Date 06 April 2022