Journal of Isfahan Medical School

Journal of Isfahan Medical School

The Genotyping of MSP-3α Gene of Plasmodium Vivax in Patients with Malaria by Nested Polymerase Chain Reaction Technique

Document Type : Original Article (s)

Authors
Sepideh Tolouei 1
Seyed Amirreza Zahir-Mirdamadi 2
Seyed Hossein Hejazi 3
Zahra Ghayour-Najafabadi 1
1 Assistant Professor, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Iran
2 MSc Student, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Iran
3 Professor, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Iran
10.22122/jims.v38i600.13330
Abstract
Background: Genotyping study and genetic diversity increase basic information about the parasites that cause malaria disease. These studies may support new treatment for disease, and control program in the future. Therefore, the present study was conducted to investigate the genetic structure and genotypes frequency based on MSP-3a gene of Plasmodium vivax in patients with Malaria reported in Isfahan City, Iran.Methods: Forty samples of patients infected with Plasmodium vivax, who were referred to laboratories of Isfahan health centers, were collected. The samples were tested using specific primers based on MSP-3a gene by nested polymerase chain reaction (PCR) method.Findings: Based on PCR product size, three genotypes of PvMSP-3a gene as A (about 1900 bp), B (about 1600 bp), and C (about 1200 bp) were observed. The most frequent genotype was genotype A with 72%, and 10% of the patients showed both genotype A and B.Conclusion: The results show that PvMSP-3α genotype can be used as a genetic marker in both endemic and imported malaria areas. This gene also is acceptable as epidemiologic marker for diagnosis of Plasmodium vivax genotyping, and detection of mixed infection having both genotypes.
Keywords
Malaria
Genotype
Plasmodium vivax
Polymerase chain reaction
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Journal of Isfahan Medical School
Volume 38, Issue 600 - Serial Number 600
September and October 2021
Pages 856-861

  • Receive Date 15 August 2020
  • Accept Date 06 April 2022