Journal of Isfahan Medical School

Journal of Isfahan Medical School

The Effect of Folic Acid and Nicotinic Acid on Malondialdehyde Levels of Semen in Oligospermia Men after Cryopreservation

Document Type : Original Article (s)

Authors
Zahra Sadeghi 1
Shahla Ishaqi 2
Gholam Reza Dashti 3
1 MSc Student, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 Laboratory Technician, St. Maryam Fertility and Infertility Center, Behesti Hospital, Isfahan University of Medical Sciences, Isfahan, Iran
3 Professor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
10.22122/jims.v38i603.13411
Abstract
Background: Reactive oxygen species (ROS) are of the most important detrimental factors on sperm quality during freezing process, which increases the lipid peroxidation (LPO) of cell membranes. In this study, the concentration of malondialdehyde (MDA) levels in semen of men with oligospermia before and after cryopreservation process was measured to evaluate the effect of folic acid and nicotinic acid on the MDA concentration after freezing.Methods: In this experimental study, semen fluid sample was collected from 25 men with oligospermia in age range of 25-45 years. Each sample was divided into 5 groups: Fresh group, Freeze group without antioxidants (control), Freeze group with nicotinic acid (10 mM), Freeze group with folic acid (50 nM), and Freeze group with a combination of nicotinic acid (10 mm) + folic acid (50 nM). The concentration of MDA in nmol/ml was measured in each group with spectrophotometer at 535 nm.Findings: The mean concentration of MDA in semen increased after freezing (1.73 ± 0.12) compared to before freezing (0.45 ± 0.02) (P < 0.001). Mean concentration of MDA in the group of folic acid + nicotinic acid (0.68 ± 0.06) was lower compared to other groups after freezing (P ± 0.001).Conclusion: The combination of folic acid and nicotinic acid antioxidants with sperm freezing medium reduces the level of MDA and LPO of sperm membrane during freezing process, and thereby maintains the fertility potential in men with oligospermia.
Keywords
Folic acid
Nicotinic acid
Malondialdehyde
Semen
Oligospermia
Cryopreservation
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Journal of Isfahan Medical School
Volume 38, Issue 603 - Serial Number 603
November and December 2021
Pages 921-928

  • Receive Date 03 September 2020
  • Accept Date 06 April 2022