Journal of Isfahan Medical School

Journal of Isfahan Medical School

Molecular Identification and Evaluation of the Ability to Produce Phospholipase and Proteinase by Aspergillus Environmental Isolates Obtained from Hospital

Document Type : Original Article (s)

Authors
Faezeh Mohammadi 1
Nima Hemmat 2
Behnaz Familsatarian 2
Asieh Maghami-Mehr 3
1 Assistant Professor, Cellular and Molecular Research Center, Research Institute for Non-Communicable Disease, Qazvin University of Medical Sciences, Qazvin, Iran
2 Medical Microbiology Research Center, Qazvin University of Medical Science, Qazvin, Iran
3 Department of Statistics, Shiraz Payame Noor University, Shiraz, Iran
10.22122/jims.v38i603.13784
Abstract
Background: One of the causes of nosocomial infections is the dispersion of Aspergillus spores in the environment. The secretion of hydrolytic enzymes is considered as a virulence factor in Aspergillus species. The aim of this study was to identify environmental Aspergillus isolates via sequencing the beta-tubulin gene and evaluating the ability to produce phospholipase and proteinase in vitro.Methods: 93 Aspergillus colonies were collected from the emergency, surgical wards, intensive care unit, and operation theatres of two teaching hospitals in Qazvin Province, Iran. The β-tubulin gene region was amplified using polymerase chain reaction (PCR) method, and 40 isolates were sequenced. Evaluation of proteinase and phospholipase production was performed using yeast carbon base (YCB) with bovine serum albumin and egg yolk agar medium, respectively.Findings: Based on β-tubulin sequence, Aspergillus (A.) flavus (30%), A. tuberculosis (25%), A. fumigatus (20%), A. niger (10%), A. sydowii (7.5%), A. terreus (5%), and A. nidulans (2.5%) were identified. Evaluation of extracellular enzymes showed that 82.5% of the isolates had proteinase ability with a mean proteinase of 0.73 ± 0.13, and 52.5% of the studied Aspergillus isolates had phospholipase activity with a mean of 0.81 ± 0.17.Conclusion: Our study showed that environmental strains have high proteinase production. Therefore, it seems necessary to better understand the association of virulence factors with aspergillosis infection in future studies.
Keywords
Aspergillus
Tubulin
Peptide hydrolases
Phospholipase
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Journal of Isfahan Medical School
Volume 38, Issue 603 - Serial Number 603
November and December 2021
Pages 929-935

  • Receive Date 26 December 2020
  • Accept Date 06 April 2022