The Effect of High-Intensity Intermittent Exercise with Silymarin Supplementation on Liver Enzymes in Male Rats of Fatty Liver Model

Document Type : Original Article (s)

Authors

1 Instructor, Department of Physical Education, Azadshahr Branch, Islamic Azad University, Azadshahr, Iran

2 Department of Physical Education, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran

3 Assistant Professor, Department of Exercise Physiology, Payame Noor University, Tehran, Iran

Abstract

Background: Non-alcoholic fatty liver disease includes a spectrum of clinical syndromes from early steatosis to liver cirrhosis. Therefore, the aim of the present study was to investigate the effects of exercise training with silymarin supplementation on liver enzymes and weight of male fatty liver rats.Methods: In this study, 40 adult male Wistar rats weighing 159 ± 3 g were randomly divided into 5 equal groups of normal diet/saline, high-fat diet/saline, high-fat diet/silymarin supplement, high-fat diet/training/ saline, and high-fat diet/training/supplement. Modeling of fatty liver with high-fat diet containing 45% energy from carbohydrates, 41% fat, and 14% protein was performed at a daily dose of 10 g per 100 g of body weight; the control group had a normal diet. The training groups practiced intermittently for 8 weeks (5 sessions per week, each session lasting 30 minutes) at intensity close to VO2max. Silymarin and saline supplementation was gavaged as 140 mg daily per kg body weight for 2 weeks. Liver tissue sampling was performed 72 hours after exercise to evaluate liver enzymes. Data were analyzed using one-way analysis of variance and Tukey post hoc test (P <0.05).Finding: Liver alanine transaminase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) enzymes significantly reduced in the periodic training and periodic exercise/silymarin supplement groups compared to the control group; but no significant difference was observed in silymarin supplement groups compared to the control group.Conclusion: Use of silymarin supplement with exercise, especially intermittent and high-intensity exercise, can modulate inflammatory and oxidative responses, and can be a suitable method to treat patients with non-alcoholic fatty liver disease.

Keywords

References

  1. Nayebifar SH, Ghasemi E, Karimipour S. Effect of high-intensity interval training and omega-3 supplementation on liver enzymes and lipid profile of young men. Sci Sport 2020; 35(1): e1-e9.
  2. Elmieh A, Rafizadeh B, Khanbabakhani H. Effect of aerobic interval training and consumption of chicory extract on levels of liver enzymes in obese boys with non-alcoholic fatty liver. Journal of Applied Exercise Physiology 2019; 15(30): 103-14. [In Persian].
  3. Polyzos SA, Kountouras J, Mantzoros CS. Obesity and nonalcoholic fatty liver disease: From pathophysiology to therapeutics. Metabolism 2019; 92: 82-97.
  4. Inoue Y, Qin B, Poti J, Sokol R, Gordon-Larsen P. Epidemiology of obesity in adults: Latest trends. Curr Obes Rep 2018; 7(4): 276-88.
  5. Marcinko K, Sikkema SR, Samaan MC, Kemp BE, Fullerton MD, Steinberg GR. High intensity interval training improves liver and adipose tissue insulin sensitivity. Mol Metab 2015; 4(12): 903-15.
  6. Hamasaki H. Perspectives on interval exercise interventions for non-alcoholic fatty liver disease. Medicines (Basel) 2019; 6(3).
  7. Pandey G, Sahni YP. A review on hepatoprotective activity of silymarin. Int J Res Ayurveda Pharm 2011; 2(1): 75-9.
  8. Jia R, Cao L, Du J, Xu P, Jeney G, Yin G. The protective effect of silymarin on the carbon tetrachloride (CCl4)-induced liver injury in common carp (Cyprinus carpio). In Vitro Cell Dev Biol Anim 2013; 49(3): 155-61.
  9. Al-Enazi MM. Neuroprotective effect of silymarin by modulation of endogenous biomarkers in streptozotocin induced painful diabetic neuropathy. Br J Pharmacol Toxicol 2013; 4(3): 110-20.
  10. Rasool M, Iqbal J, Malik A, Ramzan HS, Qureshi MS, Asif M, et al. Hepatoprotective effects of Silybum marianum (Silymarin) and Glycyrrhiza glabra (Glycyrrhizin) in combination: A possible synergy. Evid Based Complement Alternat Med 2014; 2014: 641597.
  11. Homayounfar R, Ehrampoush E, Koohpaye S A, Meshkibaf M H, Taghizade S, Almasi A, et al . Diet-induced metabolic syndrome model in rats. J Fasa Univ Med Sci 2013; 2(4): 288-96. [In Persian].
  12. Vakili J, Amir Sasan R, Ordibazar F. Effect of 8 weeks endurance training with Chlorella Vulgaris supplementation on liver enzymes levels in women with type 2 diabetes. Med J Tabriz Univ Med Sci 2019; 40(6): 88-97. [In Persian].
  13. Shafiee A, Kordi M, Gaeini A, Soleimani M, Nekouei A, Hadidi V. The effect of eight week of high intensity interval training on expression of mir-210 and ephrinA3 mRNA in soleus muscle healthy male rats. J Arak Uni Med Sci 2014; 17(3): 26-34. [In Persian].
  14. Leandro CG, Levada AC, Hirabara SM, Manhaes-de-Castro R, De-Castro CB, Curi R, et al. A program of moderate physical training for Wistar rats based on maximal oxygen consumption. J Strength Cond Res 2007; 21(3): 751-6.
  15. Yaghmaei P, Oryan SH, Solati J, Mohammadi KH, Salari A. evaluation of anxiolytic effects of silymarin extract from Silybum marianum in rats. Sci J Kurdistan Univ Med Sci 2011; 15(4): 43-51. [In Persian].
  16. Yamazaki T, Li D, Ikaga R. Effective food ingredients for fatty liver: Soy protein beta-conglycinin and fish oil. Int J Mol Sci 2018; 19(12): 4107.
  17. Motta VF, Aguila MB, Mandarim-de-Lacerda CA. High-intensity interval training beneficial effects in diet-induced obesity in mice: adipose tissue, liver structure, and pancreatic islets. Int J Morphol 2016; 34(2): 684-91.
  18. Davoodi m, Moosavi h, Nikbakht m. The effect of eight weeks selected aerobic exercise on liver parenchyma and liver enzymes (AST, ALT) of fat liver patients. J Shahrekord Univ Med Sci 2012; 14(1): 84-90.
  19. Kawanishi N, Yano H, Mizokami T, Takahashi M, Oyanagi E, Suzuki K. Exercise training attenuates hepatic inflammation, fibrosis and macrophage infiltration during diet induced-obesity in mice. Brain Behav Immun 2012; 26(6): 931-41.
  20. Brea A, Puzo J. Non-alcoholic fatty liver disease and cardiovascular risk. Int J Cardiol 2013; 167(4): 1109-17.
  21. Nikroo H, Nematy M, Sima HR, Attarzade Hosseini SR. Effect of restricted diet with or without aerobic training program on cardio respiratory fitness and anthropometric indices in patients with non alcoholic steatohepatitis. J North Khorasan Univ Med Sci 2011; 3(3): 91-9. [In Persian].
  22. Taghvaei T, Bahar A, Hosseini V, Maleki I, Kasrai M. Efficacy of silymarin on treatment of nonalcoholic steatohepatitis. J Mazandaran Univ Med Sci 2013; 23(98): 164-71. [In Persian].
  23. Takhshid MA, Rosta A, Tavasouli AR, Khabaz Z. Protective Effects of silymarin on acetic acid-induced colitis in rats. J Mazandaran Univ Med Sci 2011; 21(84): 53-61. [In Persian].
  24. Hackett ES, Twedt DC, Gustafson DL. Milk thistle and its derivative compounds: A review of opportunities for treatment of liver disease. J Vet Intern Med 2013; 27(1): 10-6.
  25. Sherif IO, Al-Gayyar MM. Antioxidant, anti-inflammatory and hepatoprotective effects of silymarin on hepatic dysfunction induced by sodium nitrite. Eur Cytokine Netw 2013; 24(3): 114-21.
Journal of Isfahan Medical School
Volume 38, Issue 606 - Serial Number 606
November and December 2021
Pages 989-995

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History

  • Receive Date: 02 November 2020
  • Revise Date: 27 April 2024
  • Accept Date: 06 April 2022

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