Journal of Isfahan Medical School

Journal of Isfahan Medical School

The Prevalence of Methicillin Resistance and the Presence of mecA and pvl Genes in Staphylococcus aureus Isolated from Valiasr Hospital, Tabriz, Iran

Document Type : Original Article (s)

Authors
Sara Naebi 1
Mehdi Ghiamirad 2
1 Department of Microbiology, Faculty of Science, Islamic Azad University, Ahar Branch, Ahar, Iran
2 Assistant Professor, Department of Microbiology, Faculty of Science, Islamic Azad University, Ahar Branch, Ahar, Iran
10.22122/jims.v39i645.14495
Abstract
Background: Methicillin-resistant Staphylococcus aureus (MRSA) strains are known as a health problem in the world due to their resistance to most antibiotics. Pantone-valentine leucocidin (PVL) is one of the important toxins of this bacterium. The aim of this study was to determine the frequency of methicillin resistance, and to investigate mecA and pvl genes in Staphylococcus aureus isolates from Valiasr hospital in Tabriz, Iran.Methods: In this cross-sectional study during 2009-2010, 142 Staphylococcus isolates were collected from hospitalized patients and identified using standard laboratory tests. Isolate resistance to common antibiotics in the treatment of staphylococcal infections was evaluated by disk diffusion method, and resistance to methicillin was investigated by cefoxitin disk method. The presence of mecA and pvl genes in the isolates was evaluated using specific primers and polymerase chain reaction (PCR) method.Findings: Of the studied isolates, 98 isolates were identified as Staphylococcus aureus. The highest antibiotic resistance level was observed against penicillin with 96.2%, and the lowest was determined against cotrimaxazole with 16.0%. Multidrug resistance (MDR) was observed in 63% of isolates. 26 isolates were identified as methicillin-resistance by phenotypic method. The mecA gene was detected in 13 isolates, of which 11 isolates were detected resistant and 2 isolates were sensitive to methicillin in the phenotypic tests. The pvl gene was not detected in any of the isolates.Conclusion: The presence of high and multidrug-resistance to common antibiotics and the presence of methicillin-resistant isolates of Staphylococcus aureus in different wards of the hospital should be considered as a serious issue.
Keywords
Staphylococcus aureus
Methicillin resistance
Nosocomial infections
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  • Receive Date 30 August 2021
  • Accept Date 06 April 2022