Journal of Isfahan Medical School

Journal of Isfahan Medical School

The Effect of Interval Training with Two Different Intensities on Adipose Tissue Triglyceride Lipase and Perilipin5 in Type 2 Diabetic Male Rats

Document Type : Original Article (s)

Authors
Roya Besharati 1
Ramin Shabani 2
Shahram Gholamrezaei 3
1 PhD Candidate, Department of Physical Education and Sports Science, Rasht Branch, Islamic Azad University, Rasht, Iran
2 Professor, Department of Physical Education and Sports Science, Rasht Branch, Islamic Azad University, Rasht, Iran
3 Assistant Professor, Department of Physical Education and Sports Science, Rasht Branch, Islamic Azad University, Rasht, Iran
10.48305/jims.v40.i667.0240
Abstract
Background: There is limited information on the effect of types of training on important regulators of fat storage and lipolysis. Therefore, the aim of the present study was to investigate the effect of interval training at two different intensities on adipose tissue triglyceride lipase and perilipin5 visceral fat tissue of type 2 diabetic male rats.
Methods: Thirty-two male Wistar rats were divided into two groups: healthy and diabetic, and then diabetic rats were randomly divided into three subgroups of diabetic control and two groups HIIT and MIIT thus, the present study was performed on four groups (8 groups each). The training was performed in MIIT with 65-70% and in HIIT group with 85-90% of maximum oxygen consumption during eight weeks. Then 24 hours after the last training session, the adipose tissue of the mice were removed using a combined intraperitoneal injection consisting anesthetic ketamine and xylazine. Expression of perilipin 5 and ATGL protein was detected via Western blotting. Data were analyzed by one-way ANOVA and Tukey's post hoc test.
Findings: The results of the post hoc test showed a significant decrease in perilipin 5 in the HIIT group and MIIT compared to the control diabetic group. Meanwhile no overall significant difference was observed between the HIIT and MIIT groups. Also, the results of a one-way analysis of variance showed that there was no significant difference in ATGL values between the research groups.
Conclusion: Both HIIT and MIIT protocols can lead to significant decrease in the tissue concentrations of perilipin 5 in type 2 diabetic rats.
Keywords
Diabetes
Perilipin 5
High-Intensity Interval Training
ATGL-1 protein
C elegans
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Journal of Isfahan Medical School
Volume 40, Issue 667
3rd Week, June
May and June 2022
Pages 240-247

  • Receive Date 22 June 2022