Journal of Isfahan Medical School

Journal of Isfahan Medical School

The Effect of Dental Stem Cell Conditioned Medium on the Thickness of the Damaged Retina in Rat

Document Type : Original Article (s)

Authors
Alireza Dehghani 1
Mohammad Malekahmadi 1
Fereshteh Karamali 2
Sareh Soroushzadeh 2
Heshmatollah Ghanbari 1
Ardeshir Talebi 3
Mohammad Hossein Nasr Esfahani 2
1 Professor, Isfahan Eye Research Center, Department of Ophthalmology, Isfahan University of Medical Sciences, Isfahan, Iran
2 Department of Animal Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
3 Department of Pathology, Isfahan University of Medical Sciences, Isfahan, Iran
10.48305/jims.v40.i668.0256
Abstract
Background: Mesenchymal stem cells and their secretions contain various factors that have anti-inflammatory properties. The aim of this study was to evaluate the effect of environment derived from stem cell from apical papilla (SCAP) on H2O2-induced retinal toxicity in rats.
Methods: Twenty healthy rats were selected for this study, all of which underwent intravitreal H2O2 injection in days 1 and 2 of the right eye. They were then randomly divided into two groups: no other intervention was performed in the first group, but in the second group, on the fifth day, the conditioned medium adjacent to the stem cell of apex of the dental papilla were injected into the vitreous of the right eye. The left eye was considered as the control. After two weeks, all rats were euthanized and histological evaluation was performed.
Findings: Retinal thickness in the second group (236.8 ± 20.8 microns) was closer to the control group compared to the first group (199.9 ± 65.3 microns) which was statistically significant.
Conclusion: This study showed that the Conditioned medium adjacent to SCAP contains secretory factors that can have a protective effect on damaged retinal cells and reduce the rate of retinal atrophy.
Keywords
Dental Papilla
Retina
Stem cells
Hydrogen peroxide
Conditioned medium
  1. Tsang SH, Sharma T. Drug-induced retinal toxicity. Adv Exp Med Biol 2018; 1085: 227-32.
  2. Krigel A, Berdugo M, Picard E, Levy-Boukris R, Jaadane I, Jonet L, et al. Light-induced retinal damage using different light sources, protocols and rat strains reveals LED phototoxicity. Neuroscience
    2016; 339: 296-307.
  3. Chang HM, Hung KH, Hsu CC, Lin TC, Chen SY. Using induced pluripotent stem cell-derived conditional medium to attenuate the light-induced photodamaged retina of rats. J Chin Med Assoc 2015; 78(3): 169-76.
  4. Ezquer M, Urzua CA, Montecino S, Leal K, Conget P, Ezquer F. Intravitreal administration of multipotent mesenchymal stromal cells triggers a cytoprotective microenvironment in the retina of diabetic mice. Stem Cell Res Ther 2016; 7(1): 42.
  5. Jin ZB, Gao ML, Deng WL, Wu KC, Sugita S, Mandai M, et al. Stemming retinal regeneration with pluripotent stem cells. Prog Retin Eye Res 2019; 69: 38-56.
  6. Duarte DA, Papadimitriou A, Gilbert RE, Thai K, Zhang Y, Rosales MA, et al. Conditioned medium from early-outgrowth bone marrow cells is retinal protective in experimental model of diabetes. PLoS One 2016; 11(2): e0147978.
  7. Kumar P, Kandoi S, Misra R, Vijayalakshmi S, Rajagopal K, Verma RS. The mesenchymal stem cell secretome: a new paradigm towards cell-free therapeutic mode in regenerative medicine. Cytokine Growth Factor Rev 2019; 46: 1-9.
  8. Jha KA, Pentecost M, Lenin R, Klaic L, Elshaer SL, Gentry J, et al. Concentrated conditioned media from adipose tissue derived mesenchymal stem cells mitigates visual deficits and retinal inflammation following mild traumatic brain injury. Int J Mol Sci 2018; 19(7): 2016.
  9. Öner A. Stem cell treatment in retinal diseases: recent developments. Turk J Ophthalmol 2018; 48(1): 33-8.
  10. Kolar MK, Itte VN, Kingham PJ, Novikov LN, Wiberg M, Kelk P. The neurotrophic effects of different human dental mesenchymal stem cells. Sci Rep 2017; 7(1): 12605.
  11. Kang J, Fan W, Deng Q, He H, Huang F. Stem cells from the apical papilla: a promising source for stem cell-based therapy. Biomed Res Int 2019; 2019: 6104738.
  12. Shin S, Lee J, Kwon Y, Park KS, Jeong JH, Choi SJ, et al. Comparative proteomic analysis of the mesenchymal stem cells secretome from adipose, bone marrow, placenta and wharton’s jelly. Int J Mol Sci 2021; 22(2): 845.
  13. Zhang M, Zhang F, Sun J, Sun Y, Xu L, Zhang D, et al. The condition medium of mesenchymal stem cells promotes proliferation, adhesion and neuronal differentiation of retinal progenitor cells. Neurosci Lett 2017; 657: 62-8.
  14. Yu S, Zhao Y, Ma Y, Ge L. Profiling the secretome of human stem cells from dental apical papilla. Stem Cells Dev 2016; 25(6): 499-508.
  15. Huang B, Liang JJ, Zhuang X, Chen SW, Ng TK, Chen H. Intravitreal injection of hydrogen peroxide induces acute retinal degeneration, apoptosis, and oxidative stress in mice. Oxid Med Cell Longev 2018; 2018: 5489476.
  16. Reisenhofer MH, Balmer JM, Enzmann V. What can pharmacological models of retinal degeneration tell us? Curr Mol Med 2017; 17(2): 100-7.
  17. Dinc E, Ayaz L, Kurt AH. Protective effect of combined caffeic acid phenethyl ester and bevacizumab against hydrogen peroxide-induced oxidative stress in human RPE cells. Curr Eye Res 2017; 42(12): 1659-66.
  18. Sugitani S, Tsuruma K, Ohno Y, Kuse Y, Yamauchi M, Egashira Y, et al. The potential neuroprotective effect of human adipose stem cells conditioned medium against light-induced retinal damage. Exp Eye Res 2013; 116: 254-64.
  19. Mead B, Logan A, Berry M, Leadbeater W, Scheven BA. Paracrine-mediated neuroprotection and neuritogenesis of axotomised retinal ganglion cells by human dental pulp stem cells: comparison with human bone marrow and adipose-derived mesenchymal stem cells. PloS One 2014; 9(10): e109305.
  20. Alsaeedi HA, Koh AEH, Lam C, Abd Rashid MB, Harun MHN, Saleh MFBM, et al. Dental pulp stem cells therapy overcome photoreceptor cell death and protects the retina in a rat model of sodium iodate-induced retinal degeneration. J Photochem Photobiol B 2019; 198: 111561.
  21. De Almeida JFA, Chen P, Henry MA, Diogenes A. Stem cells of the apical papilla regulate trigeminal neurite outgrowth and targeting through a BDNF-dependent mechanism. Tissue Eng Part Aedws 2014; 20(23-24): 3089-100.
Journal of Isfahan Medical School
Volume 40, Issue 668
4th Week, June
May and June 2022
Pages 256-261

  • Receive Date 03 July 2022