Journal of Isfahan Medical School

Journal of Isfahan Medical School

Comparing the Effect of Six Weeks of Aerobic and Resistance Training on Expression miR-195 in Male Rats with Diabetic Cardiomyopathy

Document Type : Original Article (s)

Authors
Majid Kashef 1
Mojtaba Salehpour 2
Fereshteh Shahidi 2
Nematallh Nejatmand 3
1 Professor, Department of Sports Physiology, Shahid Rajaee Teacher Training University, Tehran, Iran
2 Associate Professor, Department of Sports Physiology, Shahid Rajaee Teacher Training University, Tehran, Iran
3 PhD Student, Cardiovascular and Respiratory Field, Shahid Rajaee Teacher Training University, Tehran, Iran
10.48305/jims.v40.i704.1128
Abstract
Background: The main cause of death in diabetic patients is cardiomyopathy. Regular exercise improves insulin resistance and reduces the mortality of diabetic patients. The aim of this study was to compare the effect of six weeks of aerobic and resistance training on the expression of miR195 (specific to the myocardium) in male rats with diabetic cardiomyopathy.
Methods: Male rats with an average weight of 200 ± 20 grams and age of 8 weeks were randomly divided into four groups of 6 (sham, control, resistance and aerobic training) after the induction of diabetes (50 mg/kg STZ). A healthy group (n = 6) was also considered for comparison with the diabetic group. Exercise protocols were performed for 6 weeks. The expression level of miR195 was evaluated using PCR-Real Time. One Way Anova and T-correlated statistical tests were used for data analysis.
Findings: Both aerobic and resistance protocols led to a significant decrease in fasting blood glucose and insulin resistance index (HOMA-IR). Vvo2peak of the aerobic group and 1RM of the resistance group increased significantly. The expression of miR195 in the diagnostic and solar control groups was more different than the healthy group, but it was less in the exercise groups.
Conclusion: Compared to aerobic exercise, resistance training had a better effect on the expression of miR195 and diabetes indicators. Therefore, it is recommended that patients with cardiomyopathy should focus on resistance training.
Keywords
Exercise
Resistance training
Diabetic cardiomyopathies
MicroRNAs
  1. Roozbayani M, Peeri M, Agha-Alinejad H, Azarbayjani M A. Type of aerobic training effect on cardiac muscles MIR29A and collagen I gene expression in diabetic male rats [in Persian]. Iranian Journal of Diabetes and Obesity 2016; 8(4): 183-90.
  2. Mohammadi R, Matin Homaee H, Azarbayjani MA, Baesi K. The effect of 12 weeks of resistance training on cardiac hypertrophy, glucose, insulin and insulin resistance index in STZ-treated diabetic rats [in Persian]. Qom Univ Med Sci J 2016; 11(2): 38-45.
  3. Sanches I, Buzin M, Fernandes Conti F, da Silva Dias D, Paixão Dos Santos C, Sirvente R, et al. Combined aerobic and resistance exercise training attenuates cardiac dysfunctions in a model of diabetes and menopause. PLoS One 2018; 13(9): e0202731.
  4. Sheng Lew J, Pearson JT, Schwenke DO, Katare R. Exercise mediated protection of diabetic heart through modulation of microRNA mediated molecular pathways. Cardio Diabetology 2017; 16(10): 1-20.
  5. Khakdan S, Delfan M, Heydarpour Meymeh M, Kazerouni F, Ghaedi H, Shanaki M, et al. High-intensity interval training (HIIT) effectively enhances heart function via miR-195 dependent cardiomyopathy reduction in high-fat highfructose diet-induced diabetic rats. Arch Physiol Biochem 2018; 126(3): 250-7.
  6. Seo DY, Ko JR, Jang JE, Kim TN, Youm JB, Kwak HK, et al. Exercise as a potential target for diabetic cardiomyopathy: insight into the underlying mechanisms. Int J Mol Sci 2019; 20(24): 6284. 
  7. Delfan M, Kurdi MR, Ravasi AS, Safa M, Nasli Esfahani E, Rambod K. The effect of a period of intense intermittent and continuous endurance training on Mir1 and IGF1 gene expression in cardiomyocytes of diabetic male rats [in Persian].
    JSBio 2014; 13(1): 11-23.
  8. Dlouha D, Hubacek JA. Regulatory RNAs and Cardiovascular Disease- With a Special Focus on Circulating MicroRNAs. Physiol Res 2017; 66(1): S21-S38.
  9. Tang X, Tang G, Ozcan S. Role of microRNAs in diabetes. Biochim Biophys Acta 2008; 1779(11): 697-701.
  10. Ambros V. The functions of animal microRNAs. Nature 2004; 431(7006): 350-5.
  11. Zhou Q. MicroRNAs in diabetic cardiomyopathy and clinical perspectives. Front Gene 2014; 5: 185-97.
  12. Westermeier F, Riquelme JA, Pavez M, Garrido V, Diaz A, Verdejo HE, et al. New Molecular Insights of Insulinin Diabetic Cardiomyopathy. Front Physiol 2016; 7(125): 3-13.
  13. Guo R, Nair S. Role of microRNA in diabetic cardiomyopathy: from mechanism to intervention. Biochim Biophys Acta Mol Basis Dis 2017; 1863(8): 2070-7.
  14. Ghosh N, Katare R. Molecular mechanism of diabetic cardiomyopathy and modulation of microRNA function by synthetic oligonucleotides. Cardiovasc Diabetol 2018; 17(1): 43.
  15. Wei M, Gibbons LW, Kampert JB, Nichaman MZ, Blair SN. Low cardiorespiratory fitness and physical inactivity as predictors of mortality in men with type 2 diabetes. Ann Intern Med 2000; 132(8): 605-11.
  16. Zanuso S, Jimenez A, Pugliese G, Corigliano G, Balducci S. Exercise for the management of type 2 diabetes: a review of the evidence. Acta Diabetol 2010; 47(1): 15-22. 
  17. Wang Y, Wisloff U, Kemi OJ. Animal models in the study of exercise-induced cardiac hypertrophy. Physiol Res 2010; 59(5): 633-44.
  18. Badalzadeh R, Shaghaghi M, Mohammadi M, Dehghan G, Mohammadi Z. The effect of cinnamon extract and longterm aerobic training on heart function, biochemical alterations and lipid profile following exhaustive exercise in male rats. Adv Pharm Bull 2014; 4(Suppl 2): 515-20.
  19. Kong SW, Bodyak N, Yue P, Liu Z, Brown J, Izumo S, et al. Genetic expression profiles during physiological and pathological cardiac hypertrophy and heart failure in rat. Physiol Genomics 2005: 21(1): 34-42.
  20. Eimen-Shahidi M, Hosseinzadeh H. Animal models of diabetes [in Persian]. Journal of Diabetes and Metabolic Disorders 2003; 2(1): 1-10.
  21. Tanoorsaz S, Behpoor N, Tadibi V. Changes in Cardiac Levels of Caspase 8, Bcl2 and NT – proBNP Following 4 Weeks of Aerobic Exercise in Diabetic Rats. Int J Basic Sci Med 2017; 2(4): 172-7.
  22. Karimian J, Khazaei M, Shekarchizadeh P. Effect of resistance training on capillary density around slow and fast twitch muscle fibers in diabetic and normal rats. Asian J Sports Med 2015; 6(4): e24040.
  23. de Carvalho Picoli C, da Silva Romero PV, Gilio GR, Guariglia DA, Tófolo LP, de Moraes SMF, et al. Peak velocity as an alternative method for training prescription in mice. Front Physiol 2018; 9: 42.
  24. Molanouri Shamsi M, Hassan ZM, Mahdavi M, Gharakhanlou R, Azadmanesh K, Baghersad L, et al. Influence of Resistance Training on IL-15 mRNA Expression and the Protein Content in Slow and Fast Twitch Muscles of Diabetic Rats [in Persian]. Iranian Journal of Endocrinology and Metabolism 2012; 14(2): 185-92.
  25. Antunes LC, Elkfury JL, Jornada MN, Foletto KC, Bertoluci MC. Validation of HOMA-IR in a model of insulin resistance induced by a high-fat diet in Wistar rats. Arch Endocrinol Metab 2016; 60(2): 138-42.
  26. Nourzad F, Shahidi F, Saleh pour M. The effect of aerobic and resistance training on insulin resistance index (HOMA-IR) and BCL-2/BAX ratio in apoptotic pathway in the heart tissue of male wistar diabetic rats [in Persian]. Journal of Sport and Exercise Physiology. 2021; 15(1): 69-82.
  27. Zheng D, Ma J, Yu Y, Li M, Ni R, Wang G, et al. Silencing of miR-195 reduces diabetic cardiomyopathy in C57BL/6 mice. Diabetologia 2015; 58(8): 1949-58.
  28. Amini A, Parto P, Yousufvand N. Investigating the effect of diabetes induced by streptozotocin and treatment with zinc and vanadium sulfate on the reproductive system in rat [in Persian]. J Urmia Univ Med Sci 2015; 27(6): 476-85.
  29. Wang H, Bei Y, Lu Y, Sun W, Liu Q, Wang Y, et al. Exercise prevents cardiac injury and improves mitochondrial biogenesis in advanced diabetic cardiomyopathy with PGC-1a and Akt activation. Cell Physiol Biochem 2015; 35(6): 2159-68.
  30. Ramzany N, Gaeini A, Choobineh S, Kordi M, Hedayati M. Changes in RBP-4 and insulin resistance after 8 weeks of aerobic exercise in male type 2 diabetic rats [in Persian]. Metabolism and Exercise 2016; 5(2): 89-98.
  31. Papait R, Serio S, Condorelli G. Role of the Epigenome in Heart Failure. Physiol Rev 2020; 100(4): 1753-77.
  32. Guo R, Nair S. Role of microRNA in diabetic cardiomyopathy: from mechanism to intervention. Biochim Biophys Acta 2017; 1863(8): 2070-7.
  33. Veeranki S, Givvimani S, Kundu S, Metreveli N, Pushpakumar S, Tyagi SC. Moderate intensity exercise prevents diabetic cardiomyopathy associated contractile dysfunction through restoration of mitochondrial function and connexin 43 levels in db/db mice. J Mol Cell Cardiol 2016; 92: 163-73.
  34. Rawal S, Manning P, Katare R. Cardiovascular microRNAs: as modulators and diagnostic biomarkers of diabetic heart disease. Cardiovasc Diabetol 2014; 14; 13-44.
  35. Doresa H, Freitasd A, Malhotra A, Mendes M, Sharma S. The hearts of competitive athletes: An up-to-date overview of exercise-induced cardiac adaptations. Rev Port Cardiol 2015; 34(1): 51-64.
  36. Gaeeni A, Hemmatifar M, Toloeeazar J. An introduction to the activities of molecular sports physiology [in Persian]. Tehran, Iran: Samt Publications; 2016. p. 96-150.
Journal of Isfahan Medical School
Volume 40, Issue 704
4th Week, March
March and April 2023
Pages 1128-1137

  • Receive Date 21 December 2022
  • Revise Date 03 March 2023
  • Accept Date 04 March 2023