Journal of Isfahan Medical School

Journal of Isfahan Medical School

Investigating the Expression of HMGA-1 and HMGA-2 Genes in Tumor and Normal Samples (Tumor Margin) of Gastric Cancer in Khuzestan

Document Type : Original Article (s)

Authors
Mohammadamin Nadafi 1
Atousa Moradzadegan 2
1 MSc, Department of Experimental Sciences, Dezful Branch, Islamic Azad University, Dezful, Iran
2 Assistant Professor, Department of Experimental Sciences, Dezful Branch, Islamic Azad University, Dezful, Iran
10.48305/jims.v42.i780.0735
Abstract
Background: Stomach cancer is one of the most common malignancies worldwide, which leads to high mortality. The HMGA (high mobility group) family consists of chromatin structures that regulate proteins and play an meaningful role in tumorigenesis. This study was conducted to investigate the expression changes of these proteins in the patient population of Khuzestan, Iran.
Methods: In this survey, 60 tissue samples, including 30 gastric cancer tumor samples and 30 non-tumor samples (tumor margins), were prepared from the tumor tissue bank of Imam Khomeini Hospital (RA) in Ahvaz. After extracting RNA and evaluating its quantity and quality using a nanodrop device and agarose gel electrophoresis, the expression of HMGA-1 and HMGA-2 genes was assessed with a Real-time PCR technique. Statistical analysis was performed using t-test and ANOVA statistical methods.
Findings: The results showed that the relative expression of the HMGA-1 gene in tumor samples compared to non-tumor samples increased significantly by 17 times (P = 0.0001). Similarly, HMGA-2 gene expression in tumor samples significantly increased 15 times compared to ordinary samples (P = 0.0038).
Conclusion: This study points to the potential role of HMGA-1 and HMGA-2 in the development of gastric cancer in medical tumor samples in Khuzestan province, Iran. These findings align with previous research highlighting the roles of HMGA in various cancers, consistent with their potential role in tumorigenesis.

Highlights

Atousa Moradzadegan: Google Scholar, PubMed

Keywords
Stomach cancer
HMGA-1
HMGA-2
Clinicopathological features
Subjects
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Journal of Isfahan Medical School
Volume 42, Issue 780
4th Week, October
September and October 2024
Pages 735-741

  • Receive Date 02 August 2024
  • Accept Date 23 November 2024