Journal of Isfahan Medical School

Journal of Isfahan Medical School

Investigating the Role of T-cell-associated Lncrnas in Glioblastoma Recurrence Using Single-cell RNA Sequencing Analysis

Document Type : Original Article (s)

Authors
Morteza Hadizadeh 1
Ahmadreza Besharatnia 2
Feaze Khodamoradi Chaleshtori 2
Sorayya Ghasemi 3
1 Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
2 Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
3 Cancer Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
10.48305/jims.v43.i836.1373
Abstract
Background: Glioblastoma (GBM) is one of the primary malignant tumors of the central nervous system. Surgical limitations and resistance to therapy are important challenges in its treatment. This cancer has a high recurrence rate due to genetic heterogeneity, the presence of cancer stem cells (CSCs), and a unique tumor microenvironment (TME). The aim of this study is to identify lncRNAs associated with cells involved in recurrence and to investigate their role in regulating the immune response and related mechanisms using bioinformatic analysis of single-cell RNA data.
Methods: In this study, the differences in lncRNA expression among various cell types in primary and recurrent GBM were investigated using single-cell RNA sequencing (scRNA-seq) analysis. Data analysis was performed using the SingleR package. Cells were classified into four distinct types based on gene expression patterns. Among these, T cells were selected for further study due to their key role in the tumor microenvironment and immune response.
Findings: The different cells were categorized into 4 distinct types based on their gene expression profiles. Among these types, T cells were selected due to their pivotal role in the tumor microenvironment and immunity. The results revealed that 11 lncRNAs, including LINC01088, PVT1, and KCNQ1OT1, exhibited different expression levels in T cells between primary and recurrent GBM.
Conclusion: This study provides new insights into the differences in gene expression between primary and recurrent GBM and highlights the potential of lncRNAs as therapeutic targets. It is likely that these lncRNAs play a role in glioblastoma formation and recurrence by regulating cancer-related gene expression and pathways. Definitive and final conclusions require further complementary studies.

Highlights

Morteza Hadizadeh: PubMed

Sorayya Ghasemi:  PubMed ,Google Scholar

Keywords
Glioblastoma
RNA
Long Noncoding
Single-cell
T-Lymphocytes
Tumor microenvironment (TME)
Subjects
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Journal of Isfahan Medical School
Volume 43, Issue 836
4th Week, December
November and December 2026
Pages 1373-1382

  • Receive Date 05 February 2025
  • Accept Date 28 December 2025