Journal of Isfahan Medical School

Journal of Isfahan Medical School

Investigation of the effects of fisetin on the serum levels of interleukin 2 and 10 and prevention of cuprizone-induced myelin destruction

Document Type : Original Article(s)

Authors
Armina Bahador 1
Ebrahim Esfandiari 2
Nazem Ghasemi 3
1 MSc Student, Department of Anatomical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
2 Professor, Department of Anatomical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
3 Associate Professor, Department of Anatomical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
10.48305/jims.v43.i843.1706
Abstract
Background: Neurodegenerative diseases are among the most common inflammatory diseases that cause significant physical disabilities by disrupting the functioning of the nervous system. Fisetin, as a flavonoid, plays an important role in preventing nerve damage. In this study, the effects of fisetin on the serum levels of interleukin-2 and -10 and the prevention of cuprizone-induced myelin destruction were investigated.
Methods: 20 C57BL/6 mice were randomly assigned to control, sham, cuprizone, fisetin, and cuprizone-fisetin groups. Cuprizone was used at a dose of 400 mg/kg daily to induce demyelination. In addition, fisetin was injected intraperitoneally at a dose of 20 mg/kg in the treated groups. Finally, immunohistochemical staining was used to examine myelin and ELISA was used to assess serum levels of interleukin-2 and -10. Statistical analysis was performed using one-way ANOVA.
Findings: The results showed that the mean serum level of interleukin-2 in the cuprizone group was significantly increased compared to the other groups (P ≤ 0.01). Furthermore, the mean myelin density (P ≤ 0.001) and serum level of interleukin-10 in the fisetin group (P ≤ 0.01) and in the cuprizone-fisetin group (P ≤ 0.05) were significantly increased compared to the cuprizone group.
Conclusion: Cuprizone, with its inflammatory effects, can cause the death of oligodendrocyte cells and the destruction of myelin tissue. On the other hand, fisetin, by exerting anti-inflammatory and neuroprotective effects, can prevent the death of myelin-forming cells and maintains myelin density by reducing the destructive effects of inflammation.

Highlights

Armina Bahador: Google Scholar ,PubMed

Ebrahim Esfandiari: Google Scholar ,PubMed

Nazem Ghasemi: Google Scholar ,PubMed

Keywords
Fisetin
Myelin Sheath
Interleukin-2
Interleukin-10

Subjects

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Journal of Isfahan Medical School
Volume 43, Issue 843
3rd Week, February
January and February 2026
Pages 1706-1713

  • Receive Date 26 October 2025
  • Accept Date 05 May 2026