Journal of Isfahan Medical School

Journal of Isfahan Medical School

The Effect of a Period of High Intensity Interval Training with Gallic Acid Supplementation on Oxidative Stress in Hippocampal Tissue of Aged Male Wistar Rats

Document Type : Original Article(s)

Authors
Fatemeh Fakhri Pay Borji 1
Saeid Shakerian 2
Abdulhamid Habibi 2
Mohammad Reza Tabandeh 3
1 PhD Student, Department of Exercise Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2 Professor, Department of Exercise Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3 Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
10.48305/jims.v44.i853.0304
Abstract
Background:Aging is accompanied by an increase in oxidativestress, which is influenced by physical activity and nutritional factors.The aim of this study was to investigate the effect of 8 weeks of high-intensity interval training combined with gallic acid supplementation on oxidative stress markers, including malondialdehyde(MDA),catalase(CAT), glutathione peroxidase(GPX), superoxide dismutase(SOD), and glutathione(GSH), in the hippocampal tissue of aged mice.
Methods: Forty male 3-month-old Wistar rats (weight range: 180-200 g) were randomly divided into five groups(n=8 per group including: control healthy, control aged, aged+exercise, aged+supplement, and aged+exercise+supplement. Aging was induced by intraperitoneal injection of D-galactose at a dose of 500 mg/kg for a duration of 8 weeks.The supplement groups received galli acid by gavage at a dose of 100 mg/kg for a duration of 8 weeks. The high-intensity interval training protocol was conducted over a period of 8 weeks at an intensity of 80 to 90% VO2max.Oxidative stress markers in hippocampal tissue were measured .
Findings:Aging caused a significant decrease in antioxidant enzymes GSH, CAT, GPX, and SOD, and asignificant increase in MDAlevels (P < 0.0001). Exercise, supplementation, and the combined exercise+supplementation intervention significantly reduced MDA levelscompared tothe agedcontrolgroup (P < 0.05). Moreover,thecombined exercise + supplementation interventionsignificantly increasedantioxidant markers (GSH, CAT, GPX, SOD)compared to the aged group(P < 0.0001).
Conclusion:Highintensity interval training combined with gallicacid supplementation le to an improvement in antioxidantdefense and a reduction in lipidperoxidation inthe hippocampal tissue of aged rats.Thes findings indicate the potential interactive effect of these interventions in reducing aging-associated oxidative stress; however, generalizing the results tohumanpopulationrequires furtherstudies.

Highlights

Fatemeh Fakhri Pay Borji: Google Scholar

Saied Shakerian: Google Scholar

Keywords
Aging
Oxidative Stress
Gallic Acid
High Intensity Interval Training
Subjects
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Journal of Isfahan Medical School
Volume 44, Issue 853
1st Week, May
May and June 2026
Pages 304-313

  • Receive Date 15 July 2025
  • Accept Date 03 May 2026