Journal of Isfahan Medical School

Journal of Isfahan Medical School

Resistance to Colistin and Detection of mcr-1 Gene in Multidrug-Resistant Pseudomonas Aeruginosa Isolated from Wounds of the Hospitalized Burned Patients

Document Type : Original Article(s)

Authors
Mahsa Moarefian 1
Farkhondeh Poursina 2
Tahmineh Narimani 2
1 Master of Medical Microbiology, Department of Bacteriology and Virology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 Assistant Professor, Department of Bacteriology and Virology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
10.48305/jims.v40.i686.0678
Abstract
Background: The surge in antibiotic resistance, especially to colistin in multidrug-resistant Pseudomonas aeruginosa (MDR P. aeruginosa) is one of the important factors of mortality in wound infections in burn patients. The aim of this study was to investigate the prevalence of resistance to colistin as a last line treatment in MDR P. aeruginosa isolates from the wounds of hospitalized patients and for the detection mcr-1 gene.
Methods: Seventy-two P. aeruginosa isolates were obtained from Imam Musa Kazem Hospitals’ laboratories and confirmed using standard microbiological techniques. Antibiotic sensitivity was evaluated by the standard disk diffusion method against 8 antibiotics and minimum inhibitory concentration (MIC) of colistin was determined. The presence of mcr-1 gene was detected using specific primers by Polymerase chain reaction (PCR) test.
Findings: Out of 72 isolates, 50 (69.44%) were identified as MDR P. aeruginosa, with the highest resistance to Levofloxacin (98%) and Ciprofloxacin (92%) and the lowest. Resistance was to piperacillin tazobactam (40%). Only 2% of isolates showed complete resistance to all antibiotics. Colistin resistance was detected in 14% and mcr-1 gene in 2% of the isolates.
Conclusion: The resistance of P. aeruginosa to colistin (14%) among the isolates from burn infections in Isfahan and the prevalence of mcr-1 gene (2%) in this study, shows the importance of early detection of colistin resistance, following antibiotic resistance genes and avoid recommending this drug unnecessarily to control hospital infections.
Keywords
Wound
Pseudomonas aeruginosa
Antibiotic resistance
Colistin
mcr1
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Journal of Isfahan Medical School
Volume 40, Issue 686
2nd Week, November
November and December 2022
Pages 678-684

  • Receive Date 08 June 1401
  • Accept Date 10 July 1401