Journal of Isfahan Medical School

Journal of Isfahan Medical School

Evaluating Helicobacter Pylori FlaA in Clinical Isolates from Alzahra Hospital, Isfahan, Iran

Document Type : Original Article (s)

Authors
Masoomeh Madhi 1
Sharareh Moghim 2
Farkhondeh Pursina 3
Peyman Adibi 4
Farzad Khademi 5
Ziba Farajzadegan 6
Hajieh Ghasemian Safaei 7
1 MSc Student, Department of Microbiology, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran
2 Assistant Professor, Nosocomial Infection Research Center AND Department of Microbiology, Isfahan University of Medical Sciences, Isfahan, Iran
3 Instructor, Department of Bacteriology and Virology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
4 Professor, Gastroenterology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
5 MSc Student, Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
6 Associate Professor, Department of Community Medicine, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
7 Associate Professor, Department of Bacteriology and Virology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract
Background: This study aimed to survey the presence of flagellin gene flaA in isolated Helicobacter pylori.Methods: One hundred gastric biopsies were collected by a gastroenterologist and inoculated in enrichment medium under microaerophilic conditions. DNA was extracted and polymerase chain reaction (PCR) was performed using the designed primer.Findings: PCR products showed that all extracted DNA were positive for flaA gene.Conclusion: We found flaA gene to be present in all clinical isolates. As it is highly similar to standard Helicobacter pylori strains, it can be used to detect the mentioned bacteria. 
Keywords
flaA
Helicobacter pylori
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Journal of Isfahan Medical School
Volume 31, Issue 223 - Serial Number 223
January and February 2013
Pages 1-8

  • Receive Date 26 January 2013
  • Accept Date 06 April 2022