سلژیلین عامل افزایش تمایز سلول‌های بنیادی عصبی موش به نورون

حسن‌زاده, کامبیز; مولودی, روح‌اله; نیک‌زبان, مهرنوش; مقبل, هامون; ایزدپناه, اسماعیل

سلژیلین عامل افزایش تمایز سلول‌های بنیادی عصبی موش به نورون

نوع مقاله : مقاله های پژوهشی

نویسندگان

¹ استادیار، مرکز تحقیقات علوم سلولی و مولکولی، دانشگاه علوم پزشکی کردستان، سنندج، ایران

² استادیار، گروه فیزیولوژی و فارماکولوژی، دانشگاه علوم پزشکی کردستان، سنندج، ایران

³ کارشناس ارشد، مرکز تحقیقات علوم سلولی و مولکولی، دانشگاه علوم پزشکی کردستان، سنندج، ایران

⁴ دانشجوی پزشکی، کمیته‌ی تحقیقات دانشجویی، دانشگاه علوم پزشکی کردستان، سنندج، ایران

چکیده

مقدمه: مطالعه‌ی تأثیر عوامل مختلف بر تمایز سلول‌های بنیادی عصبی، به دلیل قابلیت استفاده از این سلول‌ها در درمان بیماری‌های نورودژنراتیو از اهمیت ویژه‌ای برخوردار است. در این مطالعه تأثیر سلژیلین بر میزان تمایز سلول‌های بنیادی عصبی موش مورد بررسی قرار گرفت.روش‌ها: سلول‌های بنیادی عصبی از دیواره‌ی بطن‌های جانبی مغز موش‌های نر 3-2 ماهه نژاد 57C جداسازی شد. به منظور ارزیابی اثر سلژیلین بر درصد تمایز سلول‌های بنیادی عصبی به نورون، آستروسیت و الیگودندروسیت از تکنیک ایمونوسیتوشیمی استفاده شد. سلول‌های بنیادی عصبی در معرض غلظت‌های مختلف سلژیلین (9-10، 8-10، 7-10 و 6-10 مولار) با دوره‌ی زمانی 7 روزه قرار داده شدند. در پایان این دوره، نمونه‌ها در معرض آنتی بادی‌های اختصاصی بر علیه نورون (β tubulin)، آستروسیت (GFAP یا Glial fibrillary acidic protein) و الیگودندروسیت (OSP یا Oligodendrocyte-specific protein) قرار گرفتند. تعداد سلول‌های تمایز یافته، شمارش و به صورت درصد نسبت به کل سلول‌ها گزارش شد.یافته‌ها: سلژیلین سلول‌های β tubulin مثبت را در غلظت‌های 9-10، 8-10 و 7-10 مولار افزایش و سلول‌های GFAP مثبت را در غلظت 6-10 مولار به طور معنی‌داری نسبت به شاهد کاهش داد.نتیجه‌گیری: سلژیلین میزان تمایز به نورون را در سلول‌های بنیادی عصبی افزایش می‌دهد و احتمال می‌رود بتوان از این دارو در آماده کردن سلول‌های بنیادی عصبی جهت پیوند استفاده کرد.

کلیدواژه‌ها

عنوان مقاله [English]

Selegiline Increases the Mouse Neural Stem Cell Differentiation into Neurons

نویسندگان [English]

Kambiz Hassanzadeh ¹
Rohallah Moloudi ²
Mehrnoosh Nikzaban ³
Hamoon Moghbel ⁴
Esmael Izadpanah ¹

¹ Assistant Professor, Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran

² Assistant Professor, Department of Physiology and Pharmacology, Kurdistan University of Medical Sciences, Sanandaj, Iran

³ Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran

⁴ Student of Medicine, Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran

چکیده [English]

Background: The effect of various agents on neural stem cells differentiation, because of their ability to use in neurodegenerative diseases, has been widely considered. In this study, the effect of selegiline on mouse neural stem cells differentiation was evaluated.Methods: Neural stem cells were isolated from the subventricular zone of the brain of male C57 mice (2-3 months of age). To assay the effect of selegiline on neural stem cells differentiation into neurons, astrocytes and oligodendrocytes, immunocytochemical techniques were utilized. Neural stem cells were exposed to different concentrations of selegiline (nano to micro Molar) for 7 days. Subsequently, samples were exposed to specific antibodies against neurons (β tubulin), astrocytes (Glial fibrillary acidic protein or GFAP) and oligodendrocytes (Oligodendrocyte-specific protein or OSP). The differentiated cells were counted and reported as percent of total cells.Findings: Selegiline increased the β tubulin positive cells (0.001 to 0.1 μM) and decreased the GFAP positive cells (1 μM) compared to vehicle treated neural stem cells.Conclusion: We found that selegiline increased the differentiation of neural stem cells into neurons. Therefore, selegiline may be a reasonable choice to use in preparation of neural stem cells for transplantation.

کلیدواژه‌ها [English]

Neural stem cells
Selegiline
Differentiation

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