اثر تمرین هوازی و مصرف عصاره‌ی بنه بر سطوح پروتئین شوک گرمایی 70 و پروتئین کربونیل بافت قلب در موش‌های دیابتی شده

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 کارشناس ارشد، گروه تربیت بدنی، دانشکده‌ی تربیت بدنی و علوم ورزشی، دانشگاه بیرجند، بیرجند، ایران

2 دانشیار، گروه تربیت بدنی، دانشکده‌ی تربیت بدنی و علوم ورزشی، دانشگاه بیرجند، بیرجند، ایران

3 استادیار، مرکز تحقیقات آترو اسکلروز و عروق کرونر، دانشگاه علوم پزشکی بیرجند، بیرجند، ایران

4 دانشجوی دکتری، گروه تربیت بدنی، دانشکده‌ی تربیت بدنی و علوم ورزشی، دانشگاه بیرجند، بیرجند، ایران

چکیده

مقدمه: استفاده‌ی هم‌زمان از فعالیت‌های‌های ورزشی و دارو، به ویژه داروهای گیاهی، از رویکردهای درمانی در افراد مبتلا به دیابت به شمار می‌رود. هدف از این پژوهش، بررسی اثر 6 هفته تمرین هوازی همراه با مصرف عصاره‌ی بنه بر سطوح پروتئین شوک گرمایی 70 (70 Heat shock protein یا 70HSP) و پروتئین کربونیل (Protein carbonyl یا PC) بافت قلبی موش‌های دیابتی شده با استرپتوزوتوسین بود.روش‌ها: در این مطالعه‌ی تجربی، 40 سر موش نر نژاد ویستار، در 5 گروه قرار گرفتند. دیابت با تزریق درون صفاقی استرپتوزوتوسین (mg/kg 40) القا شد. برنامه‌ی تمرینی شامل 6 هفته تمرین هوازی روی نوار گردان (5 جلسه در هفته، هر جلسه 40 دقیقه، با سرعت 20 متر در دقیقه و با شیب 5 درجه) بود. عصاره‌ی‌ بنه نیز با دوز mg/kg 25 (5 روز در هفته) خورانده شد. 48 ساعت پس از آخرین جلسه‌ی تمرینی، موش‌ها بیهوش شدند و بافت قلب جدا شد. سطح 70HSP و پروتئین کربونیل بافت قلب به روش ELISA (Enzyme-linked immunoassay) و رنگ‌سنجی شیمیایی سنجش شدند.یافته‌ها: سطح 70HSP در گروه دیابتی شده تحت تمرین هوازی همراه با دریافت عصاره‌ی بنه، به طور معنی‌داری پایین‌تر از گروه شاهد دیابتی بود (003/0 = P). بین سطح پروتئین کربونیل گروه‌های مورد پژوهش، اختلاف معنی‌داری آماری مشاهده نشد (740/0 = P).نتیجه‌گیری: به نظر می‌رسد، تمرین هوازی همراه با مصرف گیاهان دارویی خاص، نسبت به استفاده از هر یک از این روش‌ها به تنهایی، به عنوان یک روش درمانی مکمل، رویکرد مناسب‌تری جهت کاهش عوارض قلبی ناشی از دیابت باشد.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of Aerobic Training and Pistacia Atlantica Extract on the Levels of Heat Shock Protein 70 and Protein Carbonyl in the Heart Tissue of Diabetic Rats

نویسندگان [English]

  • Arash Hasan-Zaiem 1
  • Marziyeh Saghebjoo 2
  • Mohsen Foadodini 3
  • Saber Saed-Mocheshi 4
1 Department of Physical Education, School of Physical Education and Sport Sciences, University of Birjand, Birjand, Iran
2 Associate Professor, Department of Physical Education, School of Physical Education and Sport Sciences, University of Birjand, Birjand, Iran
3 Assistant Professor, Atherosclerosis and Coronary Heart Research Center, Birjand University of Medical Sciences, Birjand, Iran
4 PhD Student, Department of Physical Education, School of Physical Education and Sport Sciences, University of Birjand, Birjand, Iran
چکیده [English]

Background: Using the combination of exercise training and medicine, especially herbal medicine, is one of the therapeutic approaches in patients with diabetes. Purpose of the present study was to assess the effect of a 6-week aerobic training together with consuming of Pistacia atlantica extract on heat shock protein 70 (HSP70) and protein carbonyl (PC) levels in the heart tissue of streptozotocin-induced diabetic rats.Methods: In this experimental study, 40 male Wistar rats were randomly divided into five groups. Diabetes was induced via intraperitoneal injection of streptozotocin (40 mg/kg). The six-week period exercise training program included aerobic training on a treadmill (five sessions per week, 40 minutes per session, with a speed of 20 m/minute and with a slope of 5 degrees). Pistacia atlantica extract was fed five days per week with a dose of 25 mg/kg. The rats were anesthetized 48 hours after the last training session, and their heart were isolated. Then, the levels of HSP70 and protein carbonyl in the heart tissue were determined using enzyme-linked immunoassay (ELISA) and chemical colorimetric method, respectively.Findings: The HSP70 level in the diabetic group under aerobic training together with receiving Pistacia atlantica extract was significantly lower than the control diabetic group (P = 0.003); but, there was no statistically significant difference in the level of protein carbonyl between the groups (P = 0.740).Conclusion: It seems that aerobic training with Pistacia atlantica extract consuming, is a better therapeutic approach than using them alone, as a complementary therapy for reducing cardiac side effects in patients with diabetes.

کلیدواژه‌ها [English]

  • Aerobic training
  • Pistacia atlantica extract
  • Heat shock protein
  • Protein Carbonyl
  • Diabetes
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