تأثیر استروژن‌درمانی بر اختلال عملکرد شناختی ناشی از تخریب هسته‌ی قاعده‌ای مگنوسلولاریس: مدل حیوانی بیماری آلزایمر

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه زیست‌شناسی، دانشکده‌ی علوم، دانشگاه شهید چمران اهواز، اهواز، ایران

2 استاد، گروه زیست‌شناسی، دانشکده‌ی علوم، دانشگاه شهید چمران اهواز، اهواز، ایران

3 استاد، مرکز تحقیقات علوم اعصاب، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران

4 دانشیار گروه زیست شناسی دانشکده علوم دانشگاه شهید چمران اهواز، اهواز، ایران

5 استادیار، گروه آمار، دانشکده‌ی علوم ریاضی و کامپیوتر، دانشگاه شهید چمران اهواز، اهواز، ایران

چکیده

مقدمه: عملکردهای شناختی با افزایش سن و به مرور زمان کاهش می‌یابد که شاید به خاطر نقص عملکرد کولینرژیک در قاعده‌ی مغز جلویی باشد. با توجه به اهمیت فیزیولوژیک تأثیر سریع استروژن، این تحقیق با هدف ارزیابی اثر 17-β استرادیول بر تخریب دو طرفه‌ی هسته‌ی قاعده‌ای مگنوسلولاریس مو‌ش‌های صحرایی نر بالغ مدل بیماری آلزایمر انجام شد.روش‌ها: در این مطالعه، 40 سر موش‌ صحرایی نر بالغ نژاد ویستار (Wistar) به شش گروه هشت‌تایی تقسیم شدند. گروه شاهد (دست نخورده)، گروه تخریب Nucleus basalis of meynert (NBM) (تخریب دو طرفه‌ی هسته‌ی قاعده‌ای مگنوسلولاریس با القای جریان الکتریکی 5/0 میلی‌آمپر به مدت 3 ثانیه)، گروه شاهد تخریب (ورود الکترود به هسته‌ی NBM بدون القای جریان الکتریکی)، گروه استروژن (تخریب NBM + 45 میکروگرم بر کیلوگرم 17-β استرادیول)، گروه روغن کنجد (تخریب NBM + 200 میکرولیتر روغن کنجد). در آزمون‌های اکتساب و یادآوری دستگاه ماز شعاعی هشت بازویی، الگوهای ورود به بازوها در هر گروه برای محاسبه‌ی انتخاب‌های درست، خطاهای حافظه‌ی کارکردی، خطاهای حافظه‌ی مرجع و زمان سپری شده در نظر گرفته شد.یافته‌ها: تخریب دو طرفه‌ی هسته‌ی NBM، کاهش حافظه‌ی فضایی در آزمون اکتساب را به شکل افزایش معنی‌داری در خطای حافظه‌ی کارکردی و مرجع (001/0 > P) و همچنین کاهش این عملکردها در آزمون یادآوری (001/0 > P) را نسبت به گروه شاهد تخریب نشان داد. تزریق 17-β استرادیول (45 میکروگرم برکیلوگرم) بعد از تخریب NBM، پارامترهای خطاهای حافظه‌ی فضایی را در دو آزمون اکتساب و یادآوری نسبت به گروه شاهد تخریب بهبود بخشید (050/0 < P).نتیجه‌گیری: تخریب الکتریکی NBM می‌تواند عملکرد حافظه‌ی فضایی را کاهش دهد. استروژن‌درمانی بعد از آسیب مغزی اختلال شناختی را بهبود داد.

کلیدواژه‌ها

عنوان مقاله [English]

Effects of Estrogen Therapy on Cognitive Performance Deficit Induced by Nucleus Basalis Magnocellularis Lesion: Animal Model of Alzheimer’s Disease

نویسندگان [English]

  • Neda Dabir 1
  • Ahmadali Moazedi 2
  • Abbas Haghparast 3
  • Lotfollah Khajepour 4
  • Mohammadreza Akhoond 5
1 PhD Student, Department of Biology, School of Sciences, Shahid Chamran University, Ahwaz, Iran
2 Professor, Department of Biology, School of Sciences, Shahid Chamran University, Ahwaz, Iran
3 Professor, Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4 Associate Professor, Department of Biology, School of sciences, Shahid Chamran University, Ahwaz, Iran
5 Assistant Professor, Department of Statistics, School of Mathematical Science and Computer, Shahid Chamran University, Ahwaz, Iran
چکیده [English]

Background: Cognitive performance appears to diminish with age and passing of time, probably due to the basal forebrain cholinergic dysfunction. Given the physiological significance of the acute effect of estrogen, this study was conducted aiming to investigate the effect of 17β-estradiol treatment on cognitive performance in nucleus basalis magnocellularis (NBM) induced lesions.Methods: In this experimental study, 40 adult male Wistar rats were divided into five different groups (8 rat in each group): Control (intact), NBM lesion group, which received electrically-induced lesion (0.5 mA, 3s) in NBM, Sham group (the electrode was impaled into the NBM with no lesion), Estrogen group (lesion+45μg/kg 17β-estradiol) and Vehicle group (NBM lesion+200μl sesame oil). Acquisition and retention testing was done using an eight-radial arm maze, in which, the patterns of arm entries in each group was recorded for calculating correct choices, working memory errors, reference memory errors and latency.Findings: Bilateral NBM lesion showed the reduction of spatial memory acquisition in the form of increased working and reference memory errors (P < 0.001). It further reduced these functions in retention testing (P < 0.001) compared to the control group. Post-lesion treatment with 45 μg/kg estrogen improved the parameters of spatial memory errors in the acquisition and retention tasks comparing to the Sham group (P > 0.05).Conclusion: Electrical NBM lesion can reduce spatial memory function. Estrogen therapy after brain injury improved cognitive disorder.

کلیدواژه‌ها [English]

  • Alzheimer’s disease
  • Basal forebrain
  • Cognitive performance
  • Nucleus Basalis Magnocellularis
  • Estrogen Therapy

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مجله دانشکده پزشکی اصفهان
دوره 34، شماره 370 - شماره پیاپی 370
فروردین و اردیبهشت 1395
صفحه 64-73

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سابقه مقاله

  • تاریخ دریافت: 10 آبان 1394
  • تاریخ بازنگری: 06 اردیبهشت 1403
  • تاریخ پذیرش: 17 فروردین 1401

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