بررسی شیوع آلودگی به کوکسیدی‌ها و تخم کرم‌ها در نمونه‌های خاک اماکن عمومی شهر اصفهان در سال‌های 95-1394

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه قارچ و انگل‌شناسی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

2 گروه قارچ و انگل‌شناسی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

3 دانشیار، گروه قارچ و انگل‌شناسی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

چکیده

مقدمه: آلودگی خاک با انگل‌ها به منزله‌ی یک خطر جدی و مهم برای ایجاد عفونت‌های انگلی در انسان و حیوانات می‌باشد. به دلیل اهمیت خاک به عنوان یک منبع مهم در انتقال انگل‌ها به انسان و حیوانات، این مطالعه جهت تعیین شیوع اشکال مختلف انگل‌ها مانند اووسیت و تخم انجام گرفت.روش‌ها: 150 نمونه‌ی خاک از 150نقطه از اماکن عمومی شهر اصفهان جمع‌آوری گردید. جداسازی انگل از خاک، با استفاده از محلول اشباع نیترات سدیم انجام گرفت. سپس، نمونه‌ها زیر میکروسکوپ بررسی شدند و ایزوله‌های مثبت، جهت انجام اسپورولاسیون و تشخیص بهتر اووسیت‌ها، در دی‌کرومات پتاسیم 3 درصد ریخته شدند. تمامی ایزوله‌ها برای بررسی تکمیلی با رنگ‌آمیزی‌های اختصاصی Acid-fast رنگ‌آمیزی و بررسی شدند.یافته‌ها: از تعداد 150 ایزوله، تعداد 84 نمونه، مثبت و سایر نمونه‌ها فاقد هر گونه آلودگی بودند. تعداد 33 ایزوله به طور هم‌زمان واجد چند آلودگی بودند. از مجموع ایزوله‌ها 2 نمونه دارای اووسیت Toxoplasma gondii، 12 نمونه دارای اووسیت Isospora، 6 نمونه آلوده به اووسیت Eimeria، 10 نمونه دارای اووسیت Cryptosporidium، 13 نمونه دارای تخم Toxocara canis، 6 نمونه دارای تخم Toxocara cati، 4 نمونه دارای تخم Toxascaris leonina و 65 نمونه نیز دارای لارو نماتودهای خاک‌زی بودند.نتیجه‌گیری: خاک به عنوان یک منبع بالقوه مهم در انتقال آلودگی‌های انگلی محسوب می‌گردد. بهداشت و جلوگیری از آلودگی محیط خاک مهم است. بر اساس یافته‌های مطالعه‌ی حاضر، درصد انگل‌های موجود در خاک شهر اصفهان کاهش قابل توجهی یافته است.

کلیدواژه‌ها

عنوان مقاله [English]

Prevalence of Coccidians and Helminthes Ova in Soil Samples from Public Places in Isfahan City, Iran, 2016

نویسندگان [English]

  • Farnaz Heshmat 1
  • Hossein Ali Yousefi 2
  • Sepideh Tolouei 2
  • Nader Pestehchian 3
1 MSc Student, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3 Associate Professor, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
چکیده [English]

Background: Soil contamination with parasites is a serious risk for parasitic infections in humans and animals. Because of the importance of soil, as an important source for transmission of parasites to humans and animals, this study was carried out to determine the prevalence of various forms of parasite oocytes and helminthes ova.Methods: A total of 150 soil samples were collected from 150 sites in public places of Isfahan City, Iran, in 2016. Isolation of parasites from the soil was performed by saturated solution of sodium nitrate. Then, the samples were observed under microscope and positive isolates to sporulation and better recognize, were poured in 3% potassium dichromate. All the isolates were studied for further investigations with acid-fast specific staining.Findings: from 150 samples, 84 were positive and the rest were free of any contamination. 33 samples had multiple simultaneous contaminations. 2 samples were positive for Toxoplasma gondii oocytes, 12 samples for Isospora sp. oocytes, 6 samples for Eimeria sp. oocytes, 10 samples for  Cryptosporidium sp. oocytes, 13 samples for Toxocara canis oocytes, 6 samples for Toxocara cati oocytes, 4 samples for Toxascaris leoninaoocytes, and 65 samples for larvae of soil nematodes.Conclusion: Soil is considered as an important potential source in transmission of parasitic infections. Environmental hygiene is required for prevention of soil pollution and control of soil-borne parasitic infections especially. According to our study, the prevalence of parasites in the soil is decreased.Background: Soil contamination with parasites is a serious risk for parasitic infections in humans and animals. Because of the importance of soil, as an important source for transmission of parasites to humans and animals, this study was carried out to determine the prevalence of various forms of parasite oocytes and helminthes ova.Methods: A total of 150 soil samples were collected from 150 sites in public places of Isfahan City, Iran, in 2016. Isolation of parasites from the soil was performed by saturated solution of sodium nitrate. Then, the samples were observed under microscope and positive isolates to sporulation and better recognize, were poured in 3% potassium dichromate. All the isolates were studied for further investigations with acid-fast specific staining.Findings: from 150 samples, 84 were positive and the rest were free of any contamination. 33 samples had multiple simultaneous contaminations. 2 samples were positive for Toxoplasma gondii oocytes, 12 samples for Isospora sp. oocytes, 6 samples for Eimeria sp. oocytes, 10 samples for  Cryptosporidium sp. oocytes, 13 samples for Toxocara canis oocytes, 6 samples for Toxocara cati oocytes, 4 samples for Toxascaris leonina oocytes, and 65 samples for larvae of soil nematodes.Conclusion: Soil is considered as an important potential source in transmission of parasitic infections. Environmental hygiene is required for prevention of soil pollution and control of soil-borne parasitic infections especially. According to our study, the prevalence of parasites in the soil is decreased.

کلیدواژه‌ها [English]

  • Prevalence
  • Coccidia
  • Helminth
  • Ova
  • Soil
  • Iran

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مجله دانشکده پزشکی اصفهان
دوره 35، شماره 431 - شماره پیاپی 431
خرداد و تیر 1396
صفحه 577-582

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سابقه مقاله

  • تاریخ دریافت: 27 بهمن 1395
  • تاریخ بازنگری: 07 اردیبهشت 1403
  • تاریخ پذیرش: 17 فروردین 1401

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