Journal of Isfahan Medical School

Journal of Isfahan Medical School

Assessment of the Expression Level of B-Cell Lymphoma 6 (BCL6) in Peripheral Blood CD38+ B Lymphocytes in Patients with Common Variable Immunodeficiency (CVID)

Document Type : Original Article (s)

Authors
Faezeh Abbasirad 1
Mazdak Ganjalikhani-Hakemi 2
Nafiseh Esmaeil 2
Shokrollah Farrokhi 3
Roya Sherkat 4
Reza Yazdani 5
Sanaz Afshar-Ghasemlou 1
1 MSc Student, Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 Assistant Professor, Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3 Assistant Professor, Department of Immunology and Allergy, The Persian Gulf Tropical Medicine Research Center, The Persian Gulf Biomedical Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
4 Associate Professor, Acquired Immunodeficiency Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
5 Assistant Professor, Primary Immunodeficiency Research Center, Tehran University of Medical Sciences, Tehran, Iran
Abstract
immunodeficiency, is a heterogeneous set of immunological abnormalities including decreased serum levels of antibodies, and impaired antibody response to infections or vaccination. The syndrome includes impaired B-cell maturation, impaired somatic hypermutation, reduced numbers of circulating memory B cells, and absent or reduced plasma cells. B cell lymphoma 6 (BCL6) is a transcription factor which is important for the evolution and proliferation of B cells. This study aimed to investigate the expression of BCL6 in peripheral blood CD38+ B lymphocytes in patients with CVID.Methods: Blood samples were collected from 14 patients with CVID under substitutive immunoglobulin (Ig) therapy before immunoglobulin infusion and 14 normal controls. Then, peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll-Hypaque density centrifugation. CD19+ B lymphocytes were purified from PBMCs by positive selection using B-cell isolation kit. Flow cytometery method was employed to determine the expression of the BCL6 in CD38+ B cells.Findings: The expression of BCL6 in CD38 +B lymphocytes was 1.51% and 0.58% in patients and healthy subjects, respectively; the difference was not statistically significant (P > 0.05).Conclusion: The results showed that there is no significant difference in the mean expression of BCL6 of the CD38+ B cells in patients with CVID, compared with control group. However, the average BCL6 expression of CD38+ B lymphocytes in patients was more than control group.
Keywords
B-lymphocytes
Plasma cells
B-Cell Lymphoma 6
CD38
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Journal of Isfahan Medical School
Volume 35, Issue 432 - Serial Number 432
March and April 2017
Pages 629-634

  • Receive Date 22 February 2017
  • Accept Date 06 April 2022