تأثیر تعاملی تمرین هوازی در آب و مکمل کروسین بر بیان ژن‌های BAX و BCL-2 کاردیومایوسیتی در رت‌های نر تیمار شده با پراکسید هیدروژن

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشجوی دکتری تخصصی، گروه فیزیولوژی ورزشی، دانشکده‌ی علوم ورزشی، دانشگاه تربیت دبیر شهید رجایی، تهران، ایران

2 استادیار، گروه فیزیولوژی ورزشی، دانشکده‌ی علوم ورزشی، دانشگاه تربیت دبیر شهید رجایی، تهران، ایران

3 دانشیار، گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه خوارزمی، تهران، ایران

4 استاد، گروه فیزیولوژی ورزشی، دانشکده‌ی علوم ورزشی، دانشگاه تربیت دبیر شهید رجایی، تهران، ایران

5 دکتری تخصصی، مرکز تحقیقات علوم پایه، شرکت هیستوژنوتک، تهران، ایران

چکیده

مقدمه: هدف از انجام پژوهش حاضر، تعیین اثر تعامل تمرین هوازی در آب و مکمل کروسین بر بیان ژن‌های BAX و BCL-2 کاردیومایوسیتی در رت‌های نر مسموم شده با 2O2H بود.روش‌ها: 36 سر رت‌ جوان نژاد ویستار با میانگین وزنی 180 تا 200 گرم به صورت تصادفی به شش گروه «شم، 2O2H، 2O2H + کروسین، 2O2H + فعالیت در آب، 2O2H + فعالیت در آب + کروسین و گروه شاهد» تقسیم شدند. فعالیت در آب 5 روز در هفته دقیقه، هر بار 60 دقیقه و به مدت شش هفته صورت گرفت. 1 میلی‌مول بر کیلوگرم 2O2H و 5/12 میلی‌مول بر کیلوگرم کروسین نیز به صورت داخل صفاقی تزریق گردید. به منظور بیان ژن‌ها از دستگاه Real time polymerase chain reaction (Real Time PCR) و برای تأیید بروز آپوپتوز از روش Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) استفاده شد. جهت تجزیه و تحلیل داده‌ها آزمون‌های Kolmogorov-Smirnov، Levene، One-Way ANOVA و Scheffe مورد استفاده قرار گرفت.یافته‌ها: القای 2O2H با افزایش معنی‌دار بیان BAX همراه بود (001/0 = P). شش هفته فعالیت در آب منجر به کاهش بیان BCL-2 (023/0 = P) و القای کروسین نیز باعث کاهش معنی‌دار بیان BAX شد. تعامل بین فعالیت‌های القای کروسین و القای 2O2H اثر کاهنده‌ای بر بیان BAX و اثر افزاینده‌ای بر بیان BCL-2 داشت که نشان دهنده‌ی تأثیر حفاظتی فعالیت منظم در کاهش آپوپتوز کاردیومایوسیتی می‌باشد.نتیجه‌گیری: فعالیت منظم در آب و همچنین، مصرف کروسین به صورت جداگانه، منجر به کاهش معنی‌داری در بیان BAX و افزایش معنی‌داری در بیان BCL-2 می‌شود. از سوی دیگر، تلفیق فعالیت منظم و کروسین تأثیر هم‌افزایی و سینرژیک در مهار بیان BAX و افزایش بیان BCL-2 در رت‌های مسموم شده با 2O2H دارد

کلیدواژه‌ها

عنوان مقاله [English]

The Interactive Effect of Forced Swimming and Crocin Supplementation on the Expression of BAX and BCL-2 Cardiomyocyte Genes in Male Rats Infected with Hydrogen Peroxide

نویسندگان [English]

  • Mina Akbari 1
  • Fereshteh Shahidi 2
  • Hamid Rajabi 3
  • Majid Kashef 4
  • Zohreh Mazaheri 5
1 PhD Student, Department of Exercise Physiology, School of Sport Sciences, Shahid Rajaee Teacher Training University, Tehran, Iran
2 Assistant Professor, Department of Exercise Physiology, School of Sport Sciences, Shahid Rajaee Teacher Training University, Tehran, Iran
3 Associate Professor, Department of Exercise Physiology, School of Physical Education and Sport Sciences, Kharazmi University, Tehran, Iran
4 Professor, Department of Exercise Physiology, School of Sport Sciences, Shahid Rajaee Teacher Training University, Tehran, Iran
5 PhD, Basic Medical Science Research Center, Histogenotech Companey, Tehran, Iran
چکیده [English]

Background: The aim of this study was to determine the interaction effect of forced swimming and crocin supplementation on the expression of BAX and BCL-2 cardiomyocyte genes in male rats poisoned by hydrogen peroxide (H2O2).Methods: 36 male Wistar rats were randomly divided into six groups of sham, H2O2, H2O2 and crocin, H2O2 and forced swimming, H2O2 and crocin and forced swimming, and control. The forced swimming protocol was 60 minutes, 5 days a week, for 6 weeks. H2O2 (1 mg/kg for 3 weeks) and crocin (12.5 Mm/kg daily with 5 cc of distilled water) were injected intraperitoneally. Real time polymerase chain reaction (PCR) device was used to assess gene expression, and apoptosis was assessed using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method. Statistical analysis was done using Kolmogorov-Smirnov, Levene, one-way ANOVA and, the Scheffe post hoc tests.Findings: H2O2 induction was associated with a significant increase in the apoptosis of BAX (P < 0.001). Six weeks of exercise in water tended to reduction in the BCL-2 expression (P = 0.023), and induction of crocin resulted in significant decrease in BAX expression. The interaction between swimming, crocin and H2O2 decreased BAX and increased BCL-2 expression, which indicated the protective effect of regular activity in reducing cardiomyocyte apoptosis.Conclusion: Forced swimming as well as the use of crocin separately led to a significant reduction in BAX gene expression and a significant increase in BCL-2 gene expression. On the other hand, the combination of exercise and crocin had synergistic and synergistic effects in inhibiting the apoptotic expression of BAX and also enhancing the expression of the anti-apoptotic gene of BCL-2 in rats poisoned by H2O2.

کلیدواژه‌ها [English]

  • Forced swimming
  • Crocin
  • BAX protein
  • BCL2 proteins
  • Hydrogen peroxide
  • Cardiomyocyte

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مجله دانشکده پزشکی اصفهان
دوره 37، شماره 525 - شماره پیاپی 525
خرداد و تیر 1398
صفحه 443-453

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سابقه مقاله

  • تاریخ دریافت: 06 آذر 1397
  • تاریخ بازنگری: 06 اردیبهشت 1403
  • تاریخ پذیرش: 17 فروردین 1401

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