جداسازی و بررسی ویژگی‌های بنیادین سلول‌های بنیادی سرطانی با نشانگر سطحی CD44+ از رده‌ی سلولی سرطانی A2780

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشجوی دکتری زیست‌شناسی سلولی مولکولی- ژنتیک، گروه بیولوژی، دانشکده‌ی علوم، دانشگاه شهید چمران اهواز، اهواز، ایران

2 دانشیار، گروه زیست‌شناسی، دانشکده‌ی علوم، دانشگاه شهید چمران اهواز، اهواز، ایران

3 استاد، گروه ژنتیک پزشکی، دانشکده‌ی علوم و مرکز تحقیقات ژنتیک پزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران

چکیده

مقدمه: سرطان تخمدان، پنجمین علت مرگ ناشی از سرطان در بین زنان جهان می‌باشد. سرطان تخمدان یک بیماری هتروژن است که از لحاظ ژنومیک و هیستوپاتولوژیک، بسیار متنوع می‌باشد. علاوه بر درمان‌های معمول که برای سرطان تخمدان انجام می‌شود، عود مجدد این بیماری مشاهده می‌شود که به علت عدم ریشه‌کنی کامل نوع خاصی از سلول های سرطانی می‌باشد. این سلول‌ها که به نام سلول‌های بنیادی سرطانی شناخته می‌شوند، دارای خواص بنیادین متفاوتی می‌باشند.روش‌ها: در مطالعه‌ی حاضر، سلول‌های بنیادی سرطانی با نشانگر سطحی CD44 از رده‌ی سلولی سرطانی A2780 تخمدان جداسازی شد و خواص مهاجرت، رشد، تمایز و بیان نشانگر بنیادی Aldehyde dehydrogenase 1 (ALDH1) در سلول‌های CD44+ جدا شده در مقایسه با سلول‌های CD44- مورد بررسی قرار گرفت. میزان بیان نشانگر سطحی CD44 در این رده‌ی سلولی، با تکنیک فلوسایتومتری مورد ارزیابی قرار گرفت. سپس، جداسازی این سلول‌ها با استفاده از تکنیک Magnetic-activated cell sorting (MACS) و میکروبیدهای اختصاصی انجام شد و برای تأیید صحت جداسازی، از تکنیک فلوسایتومتری استفاده گردید. میزان مهاجرت، رشد، درصد تمایز و میزان بیان نشانگر ALDH1 در سلول‌های CD44+ با سلول‌های CD44- مورد مقایسه قرار گرفت.یافته‌ها: سلول‌های بنیادی سرطان CD44+ در مقایسه با سلول‌های CD44-، دارای توانایی مهاجرت، تمایز به سلول‌های اپی‌تلیال در محیط کشت حاوی سرم، میزان رشد سریع‌تر و بیان بالاتر نشانگر ALDH1 بودند.نتیجه‌گیری: جداسازی سلول‌های بنیادی سرطانی برای درمان هدفمند سرطان تخمدان بسیار حایز اهمیت است و می‌تواند از عود مجدد و تکثیر مجدد تومور جلوگیری کند.

کلیدواژه‌ها

عنوان مقاله [English]

Isolation and Characterization of Stemness Properties of Cancer Stem Cells with CD44+ Surface Marker from A2780 Cancer Cell Line

نویسندگان [English]

  • Vahideh Keyvani 1
  • Seyed Reza Kazeminezhad 2
  • Mohammad Reza Abbaszadegan 3
1 PhD Candidate of Molecular Genetics, Department of Biology, School of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2 Associate Professor, Department of Biology, School of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3 Professor, Department of Medical Genetics, School of Science AND Medical Genetics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده [English]

Background: Ovarian cancer is the fifth leading cause of cancer death among women worldwide. Ovarian cancer is a heterogeneous disease that is genomically and histopathologically very diverse. In spite of usual treatments, recurrence of this cancer is very common due to the lack of complete eradication of certain types of cancer cells. These cells, known as cancer stem cells, have different stemness properties.Methods: In the present study, we isolated cancer stem cells (CSCs) with CD44 surface marker from ovarian A2780 cell line, and examined the invasion, growth, and differentiation properties and ALDH1 expression of isolated CD44+ cells compared to CD44- cells. The expression level of CD44 in this cell line was evaluated by flow cytometry (FCM) technique. Then, the isolation of these cells was performed by magnetic-activated cell sorting (MACS) technique using specific microbeads, and FCM was used to confirm the isolation efficacy. Invasion, growth rate, differentiation percentage, and expression rate of ALDH1 in CD44+ cells were evaluated and compared with CD44- cells.Findings: CD44+ CSCs, compared to CD44- cells, not only had the ability to invade other tissues, and differentiate to epithelial cells in culture medium containing serum, but also had faster growth rate and higher ALDH1expression in comparison with the control.Conclusion: Isolation of cancer stem cells has great importance in the targeted treatment of ovarian cancer and inhibition of tumor recurrence.

کلیدواژه‌ها [English]

  • Ovarian neoplasm
  • Neoplastic stem cell
  • Recurrence
  • CD44 antigen

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  • تاریخ دریافت: 17 خرداد 1400
  • تاریخ بازنگری: 06 اردیبهشت 1403
  • تاریخ پذیرش: 17 فروردین 1401

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