نوع مقاله : مقاله های پژوهشی
1 استادیار، گروه تربیت بدنی، واحد آزادشهر، دانشگاه آزاد اسلامی، آزادشهر، ایران
2 گروه تربیت بدنی، واحد آزادشهر، دانشگاه آزاد اسلامی، آزادشهر، ایران
3 استادیار ،گروه تربیت بدنی، واحد گرگان، دانشگاه آزاد اسلامی، گرگان، ایران
عنوان مقاله [English]
Background: Lymphangiogenesis plays an important role in islet inflammation and insulin regulation. In this study, the effect of interval training and liposomal alpha lipoic acid supplementation on serum glucose, blood insulin, and vascular endothelial growth factor receptor-3 (VEGFR3) was examined in diabetic rat.Methods: 25 male Wistar rats with an average age of 3 weeks, and weighing 159 ±3 g were divided in seven groups (n = 5) of control (healthy), diabetes + saline, diabetes + supplement, diabetes + exercise + saline, and diabetes + supplement + exercise. To induce diabetes, streptozotocin (65 mg/kg) was injected subcutaneously, and then nicotine amid (120 mg/kg) was injected. Alpha lipoic acid was administered orally (20 mg/kg/day) for 6 weeks. The training program included intermediate interval training, including 6 weeks of training, 5 session per week, consisted of 13 periods of 4 minutes with an intensity of 70-65 percent VO2max running on a treadmill. Active rest between intervals was in the form of 2-minute runs with an intensity of 5-10 m/minute. After killing rats and sampling, pancreatic tissue was sent to the laboratory to examination the expression of VEGFR3 protein by immunohistochemistry, glucose by calorimetric method, and insulin by enzyme-linked immunoassay (ELISA)-calorimetric method. Data were analyzed using one-way ANOVA in SPSS software at a significant level of P < 0.050.Findings: 6 weeks of interval training and interval training with liposomal alpha lipoic acid had a significant effect on VEGFR3 pancreatic tissue, glucose, and serum insulin in diabetic rats. 6 weeks of liposomal alpha lipoic acid supplementation had a significant effect on VEGFR3 and serum insulin in diabetic rats.Conclusion: Intense interval training and liposomal alpha lipoic acid supplementation increased lymphangiogenesis and decreased blood glucose and favorable changes in diabetic rats.