تأثیر هشت هفته تمرین تناوبی با شدت بالا و مصرف مکمل کورکومین بر بیان ژن‌Drp1 و MFN2 میتوکندریایی کاردیومیوسیت‌ موش‌های نر مدل سکته‌ی قلبی

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه تربیت بدنی و علوم ورزشی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران

2 استادیار، گروه تربیت بدنی و علوم ورزشی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران

3 استاد، گروه تربیت بدنی و علوم ورزشی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران

چکیده

مقدمه: هدف از انجام پژوهش حاضر، بررسی تأثیر هشت هفته تمرین تناوبی با شدت بالا (High-intensity interval training یا HIIT) و مصرف مکمل کورکومین بر بیان ژن‌های Drp1 و MFN2 میتوکندریایی کاردیومیوسیت‌ موش‌های نر مدل سکته‌ی قلبی بود.روش‌ها: در این مطالعه‌ی تجربی، 32 سر موش صحرایی نر به صورت تصادفی به چهار گروه HIIT، مکمل، شاهد و مکمل + تمرین تقسیم شدند. گروه تمرین به مدت هشت هفته تحت HIIT قرار گرفتند. تمرین در دو گروه شدت بالا و تمرین + مکمل شامل اجرای 10 مرحله فعالیت 4 دقیقه‌ای با شدت 90-85 درصد حداکثر اکسیژن اشباع بود و در این زمان گروه مکمل برنامه‌ی تمرینی نداشت. بیان ژن Drp1 و MFN2 میتوکندریایی با استفاده از روش Real-time polymerase chain reaction (RT-PCR) به دست آمد. ﺩﺍﺩﻩ‌ﻫﺎ ﺑﺎ ﺍﺳﺘﻔﺎﺩ ﺍﺯ آزمون‌های Shapiro-Wilk و One-way ANOVA مورد تجزیه و تحلیل قرار گرفت. یافته‌ها: نتایج آزمون One-way ANOVA نشان داد که بین میانگین گروه‌ها در بیان ژن Drp1 و MFN2 میتوکندریایی تفاوت معنی‌داری وجود داشت. نتایج آزمون Tukey بیان‌کننده‌ی کاهش معنی‌دار بیان ژن Drp1 در دو گروه تمرین و تمرین + مکمل نسبت به گروه مکمل و شاهد و افزایش معنی‌دار بیان ژن MFN2 در دو گروه تمرین و تمرین + مکمل نسبت به گروه مکمل و شاهد بود.نتیجه‌گیری: HIIT و مصرف کورکومین می‌تواند عملکرد میتوکندری‌های کاردیومیوسیت‌ موش‌های نر مدل سکته‌ی قلبی را از طریـق تعدیل فرایندهای هم‌جوشی و شکافت بهبود بخشد.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of 8 Weeks of High-Intensity Interval Training (HIIT) with Curcumin Supplementation on Expression of Mitochondrial Cardiomyocyte drp1 and MFN2 Genes in Male Stroke Model Rats

نویسندگان [English]

  • Mohamad-Rasoul Mirzaei 1
  • Shahram Gholamrezaei 2
  • Ramin Shabani 3
1 PhD Student, Department of Physical Education and Sports Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran
2 Assistant Professor, Department of Physical Education and Sports Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran
3 Professor, Department of Physical Education and Sports Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran
چکیده [English]

Background: This study aimed to assess the effect of 8 weeks of high-intensity interval training (HIIT) with curcumin supplementation on expression OF mitochondrial cardiomyocyte drp1 and MFN2 genes in male stroke model rats.Methods: In this experimental study, 32 male rats were randomly divided into 4 groups of HIIT, supplement, control and supplement + training. The exercise group was exposed to HIIT for 8 weeks. Training program in the two groups of HIIT and training + supplementation included performing 10 periods of 4-minute activities with an intensity of 85%-90% VO2max, and at this time, the supplementary group did not have training program. The expression of mitochondrial drp1 and MFN2 genes was obtained by real-time polymerase chain reaction (RT-PCR). Data were analyzed using one-way ANOVA and Shapiro-Wilk tests.Findings: The results of one-way ANOVA showed a significant difference between the means of all of the groups in expression of mitochondrial Drp1 and MFN2 genes. The results of Tukey test showed a significant decrease in Drp1 gene expression in both training and exercise + supplement groups compared to the supplement and control groups and a significant increase in MFN2 gene expression in both training and supplement + supplement groups compared to the supplement and control groups.Conclusion: HIIT with curcumin supplementation can improve the function of the cardiomyocytes' mitochondria of male myocardial infarction rats by modulating fusion and fission processes.

کلیدواژه‌ها [English]

  • High intensity interval training
  • Curcumin
  • Gene Expression
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