Journal of Isfahan Medical School

Journal of Isfahan Medical School

The Effect of 8 Weeks of High-Intensity Interval Training (HIIT) with Curcumin Supplementation on Expression of Mitochondrial Cardiomyocyte drp1 and MFN2 Genes in Male Stroke Model Rats

Document Type : Original Article (s)

Authors
Mohamad-Rasoul Mirzaei 1
Shahram Gholamrezaei 2
Ramin Shabani 3
1 PhD Student, Department of Physical Education and Sports Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran
2 Assistant Professor, Department of Physical Education and Sports Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran
3 Professor, Department of Physical Education and Sports Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran
10.22122/jims.v39i652.14679
Abstract
Background: This study aimed to assess the effect of 8 weeks of high-intensity interval training (HIIT) with curcumin supplementation on expression OF mitochondrial cardiomyocyte drp1 and MFN2 genes in male stroke model rats.Methods: In this experimental study, 32 male rats were randomly divided into 4 groups of HIIT, supplement, control and supplement + training. The exercise group was exposed to HIIT for 8 weeks. Training program in the two groups of HIIT and training + supplementation included performing 10 periods of 4-minute activities with an intensity of 85%-90% VO2max, and at this time, the supplementary group did not have training program. The expression of mitochondrial drp1 and MFN2 genes was obtained by real-time polymerase chain reaction (RT-PCR). Data were analyzed using one-way ANOVA and Shapiro-Wilk tests.Findings: The results of one-way ANOVA showed a significant difference between the means of all of the groups in expression of mitochondrial Drp1 and MFN2 genes. The results of Tukey test showed a significant decrease in Drp1 gene expression in both training and exercise + supplement groups compared to the supplement and control groups and a significant increase in MFN2 gene expression in both training and supplement + supplement groups compared to the supplement and control groups.Conclusion: HIIT with curcumin supplementation can improve the function of the cardiomyocytes' mitochondria of male myocardial infarction rats by modulating fusion and fission processes.
Keywords
High intensity interval training
Curcumin
Gene Expression
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Journal of Isfahan Medical School
Volume 39, Issue 652 - Serial Number 652
November and December 2022
Pages 914-921

  • Receive Date 18 October 2021
  • Revise Date 28 June 2022
  • Accept Date 06 April 2022