تشخیص انتروباکترهای تولیدکننده‌ی بتالاکتاماز با طیف گسترده و کارباپنماز در نمونه‌های بالینی: مروری

نوع مقاله : Review Article

نویسندگان

1 استادیار، مرکز تحقیقات عفونی اطفال، پژوهشکده‌ی بیماری‌های واگیر، دانشگاه علوم پزشکی مازندران، ساری، ایران

2 استاد، مرکز تحقیقات عفونی اطفال، پژوهشکده‌ی بیماری‌های واگیر، دانشگاه علوم پزشکی مازندران، ساری، ایران

چکیده

مقاله مروری



مقدمه: ظهور انتروباکترهای تولیده‌کننده‌ی کارباپنماز و بتالاکتاماز وسیع‌الطیف یک تهدید برای سلامت جهانی می‌باشد. تشخیص سریع و صحیح این سویه‌ها جهت کنترل عفونت‌های بیمارستانی و درمان صحیح نقش کلیدی دارد. در این مطالعه روش‌های تشخیص انتروباکترهای مولد کارباپنماز و بتالاکتاماز وسیع‌الطیف با تمرکز بر خلاصه تکنیک‌های مبتنی بر کشت و روش‌های مولکولی مورد بررسی قرار گرفته است.
روش‌ها: مطالعه‌ی حاضر از نوع مروری است. که در آن مقالات منتشر شده طی سال‌های 1381 تا 1401 در پایگاه‌های معتبر بین‌المللیScopus ، PubMed، Scholar Google، Science Direct، Web of Science جستجو گردید.
یافته‌ها: برای شناسایی انتروباکترهای تولیده‌کننده‌ی کارباپنماز و بتالاکتاماز وسیع‌الطیف روش‌های مرسوم کشت، روش‌های بیوشیمیایی، طیف‌سنجی جرمی، روش‌های مولکولی، توالی‌یابی نسل بعدی، ریز آرایه‌ها، توالی‌یابی کل ژنوم، سیستم مبتنی بر هیبریداسیون، سنجش ید نشاسته، ایمونوکروماتوگرافی، رنگ‌سنجی، طیف‌سنجی جرمی، سنجش الکتروشیمیایی و فلوسایتومتری مورد استفاده می‌باشند.
نتیجه‌گیری: (matrix-assisted laser desorption ionization–time of flight) MALDI–TOF، توالی‌یابی نسل بعدی و توالی‌یابی کل ژنوم از جمله تکنیک‌های نوین و منتخب جهت تشخیص و شناسایی انتروباکترهایتولیده‌کننده‌ی کارباپنماز و بتالاکتاماز وسیع‌الطیف می‌باشند.

کلیدواژه‌ها


عنوان مقاله [English]

Detection Extended-Spectrum Beta-Lactamase- and Carbapenemase-Producing Enterobacteriaceae Isolates from Clinical Samples; Narrative Review

نویسندگان [English]

  • Golnar Rahimzadeh 1
  • Mohammad Sadegh Rezai 2
1 Assistant Professor, Pediatric Infectious Diseases Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran
2 Professor, Pediatric Infectious Diseases Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran
چکیده [English]

Background: The emergence of Extended-Spectrum Beta-Lactamase- and Carbapenemase- Producing Enterobacteriaceae is a threat to global health. Fast and accurate detection of these strains plays a key role in controlling nosocomial infections and effective treatment. In the present study, the detection methods of carbapenemase and broad-spectrum β-lactam-producing Enterobacteriaceae have been investigated, focusing on the summary of culture-based techniques and molecular methods.
Methods: The present study is a narrative review. The articles published between 2000 and 2022 were searched in international authoritative databases namely Scopus, PubMed, Scholar, Google, Web of Science, Science direct.
Findings: To identify Extended-Spectrum Beta-Lactamase- and Carbapenemase-Producing Enterobacteriaceae, conventional culture methods, biochemical methods, mass spectrometry, molecular methods, next generation sequencing, microarrays, whole genome sequencing, hybridization-based system, starch iodine assay, immunochromatography, colorimetry, mass spectrometry, electrochemical measurement, and flow cytometry were used.
Conclusion: For the detection of Extended-Spectrum Beta-Lactamase- and Carbapenemase-Producing Enterobacteriaceae, MALDI-TOF (matrix-assisted laser desorption ionization-time of flight), next-generation sequencing and whole genome sequencing are among the new and selected techniques.

کلیدواژه‌ها [English]

  • Carbapenemase
  • Beta-Lactamases
  • Enterobacteriaceae
  • Phenotypic techniques
  • Molecular diagnostic technique
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