مقایسه‌ی اثر تمرین مقاومتی- استقامتی بر تعداد سلول‌های استئوسیت، استئوبلاست و استئوکلاست بافت استخوان فمور در موش‌های نر بالغ، کودک و سالمند

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشجوی دکترای تخصصی فیزیولوژی ورزشی، گروه تربیت بدنی و علوم ورزشی، دانشکده‌ی علوم انسانی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران

2 استادیار، گروه تربیت بدنی و علوم ورزشی، دانشکده‌ی علوم انسانی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران

چکیده

مقاله پژوهشی




مقدمه: اگرچه تمرین ورزشی به عنوان یک عامل مؤثر در حفظ و بهبود استحکام استخوان شناخته شده است؛ اما مشخص نیست که آثار آن بر سلول‌های استخوان‌ساز و استخوان‌خوار رده‌های سنی مختلف یکسان باشد. بنابراین هدف پژوهش حاضر، بررسی اثر تمرین مقاومتی- استقامتی بر تعداد سلول‌های استئوسیت، استئوبلاست و استئوکلاست بافت استخوان فمور در موش‌های نر با سنین مختلف بود.
روش‌ها: در مطالعه‌ی حاضر، 30 سر موش صحرایی نر نژاد ویستار در سه گروه سنی 2 هفته‌ای، 6 هفته‌ای و96 هفته‌ای به صورت تصادفی در 2 گروه 5‌تایی تمرین و شاهد قرار گرفتند. تمرینات مقاومتی- استقامتی برای 6 جلسه تمرین در هفته به مدت 6 هفته اجرا شد. موش‌ها تحت گاز دی‌اکسید کربن قربانی شدند. جهت بررسی میکروسکوپی بافتی از رنگ‌آمیزی به روش H&E استفاده شد.
یافته‌ها: نتایج آنالیز واریانس یک طرفه با آزمون تعقیبی Tukey نشان داد، مقادیر بالاتر سلول‌های استئوبلاست و استئوسیت و مقادیر پایین‌تر سلول‌های استئوکلاست موش‌های تمرین کرده در هر سه رده‌ی سنی در مقایسه با گروه شاهد خودشان، معنی‌دار بود. مقایسه‌ی گروه‌های تمرین با یکدیگر نشان داد، تعداد استئوبلاست‌ها در گروه‌های کودک و بالغ، تعداد استئوسیت‌ها در گروه بالغ و تعداد استئوکلاست‌ها در گروه‌های بالغ و سالمند بالاتر بود.
نتیجه‌گیری: نتایج نشان داد تمرین مقاومتی- استقامتی منجر به افزایش سلول‌های استخوان‌ساز و کاهش سلول‌های استخوان‌خوار شد. هرچند آثار ورزش بر استخوان‌سازی در رده‌های سنی پایین‌تر بیشتر از دوران سالمندی بود. با این وجود ممانعت ورزش از روند استخوان‌خواری در سنین بالاتر خصوصاً دوره‌ی سالمندی مشهود بود.

تازه های تحقیق

مهشیدشیری: Google Scholar

محمدرضا فدائی چافی: Google Scholar 

شهرام غلامرضایی: Google Scholar 

کلیدواژه‌ها

عنوان مقاله [English]

Comparison of the Effect of Resistance-Endurance Training on the Number of Osteocyte, Osteoblast, and Osteoclast Cells in Femur Bone Tissue in Adult, Child, and Elderly Male Rats

نویسندگان [English]

  • Mahshid Shiri 1
  • Mohammad Reza Fadaei Chafy 2
  • Shahram Gholamrezaei 2
1 PhD Candidate of Exercise Physiology, Department of Physical Education and Sport Science, Faculty of Humanities, Rasht Branch, Islamic Azad University, Rasht, Iran
2 Assistant Professor, Department of Physical Education and Sport Science, Faculty of Humanities, Rasht Branch, Islamic Azad University, Rasht, Iran
چکیده [English]

Background: Although exercise is known as an effective factor in maintaining and improving bone strength; it is not clear that its effects on bone-forming and bone-eating cells of different age groups are the same. Therefore, this study aimed to investigate the effect of resistance-endurance training on the number of osteocyte, osteoblast, and osteoclast cells in femur bone tissue in male rats of different ages.
Methods: In the present study, 30 male Wistar rats in three age groups of 2 weeks, 6 weeks, and 96 weeks were randomly divided into 2 groups of 5 each, training and control. Resistance-endurance exercises were performed for 6 training sessions per week for 6 weeks. Rats were sacrificed under carbon dioxide gas. H&E staining was used for tissue microscopic examination.
Findings: The results of one-way analysis of variance with Tukey's post hoc test showed that higher amounts of osteoblast and osteocyte cells and lower amounts of osteoclast cells in trained rats of all three age groups were significant compared to their control group. The comparison of exercise groups showed that the number of osteoblasts in the child and adult groups, the number of osteocytes in the adult group, and the number of osteoclasts in the adult and elderly groups were higher.
Conclusion: The results showed that resistance-endurance training led to an increase in bone-forming cells and a decrease in bone-eating cells. However, the effects of exercise on bone formation in younger age groups were more than in old age. Nevertheless, the prevention of exercise from the process of bone loss in older age, especially old age, was evident.

کلیدواژه‌ها [English]

  • Strength exercise
  • Aerobic exercise
  • Osteocyte
  • Osteoblast
  • Osteoclast

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مجله دانشکده پزشکی اصفهان
دوره 41، شماره 734
هفته 2، آبان
مهر و آبان 1402
صفحه 764-772

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سابقه مقاله

  • تاریخ دریافت: 14 اردیبهشت 1402
  • تاریخ پذیرش: 02 آبان 1402

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