تأثیر تمرین تناوبی پرشدت (HIIT) بر محتوای پروتئین‌ شبه داینامین 1 (DRP1) و آتروفی اپتیک 1 (OPA1) بطن چپ رت‌های نر سالمند

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 گروه تربیت بدنی و علوم ورزشی، واحد علی‌آباد کتول، دانشگاه آزاد اسلامی، علی‌آباد کتول، ایران.

2 گروه آموزش تربیت بدنی، دانشگاه فرهنگیان، صندوق پستی 889- 14665 تهران، ایران

چکیده

مقاله پژوهشی




مقدمه: پیری همراه با ضعف تدریجی عملکرد قلب است که می‌تواند منجر به افزایش خطر بیماری‌های قلبی- عروقی شود. به نظر می‌رسد تمرین تناوبی پرشدت HIIT
(High-intensity interval training)  به عنوان مداخله‌ی ورزشی قوی اثرات مفیدی بر عملکرد قلب و سلامت میتوکندری در گروه‌های سالمند دارد.
روش‌ها: پژوهش حاضر از نوع تجربی است، که با 12 سر رت نر 20 ماهه از نژاد ویستار با میانگین وزنی 30 ± 400 گرم انجام شد. معیار ورود، رت‌های نر سالمند 20 ماه و بالاتر بودند که بطور تصادفی به دو گروه HIIT و شاهد (هر گروه 6 سر) تقسیم شدند. در حین انجام تحقیق، گروه شاهد فعالیتی نداشتند و گروه تمرین به مدت 8 هفته‌ و هر هفته 5 جلسه بر روی تردمیل دویدند. برنامه‌ی HIIT با وهله‌های پرشدت 85 تا 95 درصد سرعت بیشینه (متر بر دقیقه) و دوره‌های استراحت فعال با شدت 40 تا 55 درصد سرعت بیشینه (متر بر دقیقه) برای هر جلسه در هفته انجام شد. پس از 48 ساعت از آخرین جلسه تمرین، رت‌ها بی‌هوش شدند و با روش وسترن بلات متغیرهای DRP1 و OPA1 اندازه‌گیری شدند. داده‌ها از طریق آزمون آماری Shapiro-Wilk و  Independent Sample T-test تحلیل شدند و سطح معنی‌داری 0/05 ≥ P در نظر گرفته شد.
یافته‌ها: نتایج نشان داد، 8 هفته HIIT سبب افزایش معنی‌دار DRP1 (0/001 = P) در قلب رت‌های پیر شد در حالی که محتوای پروتئین OPA1 (0/09 = P) را تغییر معنی‌دار نداد.
نتیجه‌گیری: به نظر می‌رسد پروتکول HIIT می‌تواند کنترل کیفیت میتوکندری را در قلب رت‌های سالمند بهبود بخشد.

تازه های تحقیق

ندا آقایی بهمن‌بگلو: Google Scholar, PubMed

حامد علیزاده پهلوانی: Google Scholar, PubMed

حبیب اصغرپور:  Google Scholar

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effect of High-Intensity Interval Training (HIIT) on the Contents of Dynamin-Like Protein 1 (DRP1) and Optic Atrophy 1 (OPA1) in the Left Ventricle of Aged Male Rats

نویسندگان [English]

  • Mahdi Marezloo 1
  • Neda Aghaei Bahmanbeglou 1
  • Hamed Alizadeh Pahlavani 2
  • Habib Asgharpour 1
1 Department of Physical Education and Sport Sciences, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran
2 Department of Physical Education, Farhangian University, P.O. Box 14665-889, Tehran, Iran
چکیده [English]

Background: Aging is accompanied by a gradual weakening of heart function, which can lead to an increase in the risk of cardiovascular diseases. HIIT, as a potent exercise intervention, seems to benefit heart function and mitochondrial health in elderly groups.
Methods: The present research is of an experimental type, conducted with 12 20-month-old Wistar male rats with an average weight of 400 ± 30 grams. The inclusion criteria were male rats aged 20 months and older, which were randomly divided into two HIIT and control groups (6 rats in each group). During the research, the control group had no activity, and the training group ran on the treadmill for eight weeks and five sessions weekly. The HIIT program was performed with high-intensity bouts of 85-95% of maximum speed (m/min) and active rest periods with an intensity of 40-55% of maximum speed (m/min) for each session per week. After 48 hours following the last training session, the rats were anesthetized, and the variables were measured using the western blot method. The data were analyzed through Shapiro-Wilk and independent t-test, and the significance level was considered P ≥ 0.05.
Findings: The results showed that eight weeks of HIIT caused a significant increase in DRP1 in the hearts of old rats, while it did not significantly change the content of OPA1 protein.
Conclusion: HIIT protocol seems to improve mitochondrial quality control in the heart of aged rats.

کلیدواژه‌ها [English]

  • Heart disease
  • High-intensity interval training (HIIT)
  • Left ventricle
  • Mitochondrial fission
  • Mitochondrial fusion

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مجله دانشکده پزشکی اصفهان
دوره 42، شماره 776
هفته 4، شهریور
مرداد و شهریور 1403
صفحه 615-623

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سابقه مقاله

  • تاریخ دریافت: 01 مرداد 1403
  • تاریخ پذیرش: 13 شهریور 1403

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