اثر یک وهله فعالیت ورزشی مقاومتی با دو شدت و ریکاوری متفاوت بر بیان ژن‌های TNF-αو اینترلوکین -6، hs-CRP و نشانگر ایمنی- التهابی سیستمیک SII بر زنان بدنساز

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشجوی دکترای فیزیولوژی ورزشی ،گروه تربیت بدنی و علوم ورزشی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران

2 استاد، گروه تربیت بدنی و علوم ورزشی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران

3 دانشیار، گروه تربیت بدنی و علوم ورزشی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران

چکیده

مقاله پژوهشی




مقدمه: تمرینات مقاومتی با بار زیاد (High-load resistance training) HLRT، موجب تغییرات فیزیولوژیکی در سیستم‌های بدن از جمله در سیستم ایمنی شود. از سوی دیگر تحقیقات کمی بر روی اثر تمرینات بدنسازی با شدت بالا بر سیستم ایمنی زنان انجام شده است. پژوهش حاضر اثر یک وهله فعالیت مقاومتی با دو شدت و ریکاوری متفاوت بر بیان ژن TNF-α و اینترلوکین-6، hs-CRP و نشانگر ایمنی- التهابی سیستمیک (Systemic Immune-Inflammation Index=P × N/L) SII بر زنان بدنساز مورد بررسی قرار داد.
روش‌ها: در این مطالعه‌ی نیمه تجربی، 12 زن ورزشکار بدنساز سالم (سن 5 ± 30) داوطلب شرکت در این مطالعه شدند. شرکت‌کنندگان پس از پر کردن فرم رضایت‌نامه‌ی عمل در 4 جلسه تمرینی، دو وهله‌ی تمرینی با شدت 70 و 85 درصد یک تکرار بیشینه (Repitition Maximum 1RM) را یکبار با زمان ریکاوری یک دقیقه و بار بعدی با دو دقیقه ریکاوری انجام دادند. 36 ساعت بعد از اتمام هر جلسه تمرین، پس از 10 ساعت ناشتا نمونه‌گیری انجام شد. نمونه‌ها با 3000 دور در دقیقه سانتریفیوژ و در دمای منفی ℃20- نگهداری شدند. در پایان، همه مقادیر TNF-αو اینترلوکین-6، hs-CRP و نشانگرهای ایمنی- التهابی سیستمیکSII  چهار مرحله با یکدیگر مقایسه شد. جهت تجزیه و تحلیل داده‌ها در سطح معنی‌داری 0/05 α ≤ استفاده شد.
یافته‌ها: بر اساس نتایج تمرین مقاومتی به روش HLRT در مقایسه با تمرینات مقاومتی با شدت متوسط، با افزایش شدت تمرین تا 1RM 85 درصد و ریکاوری یک دقیقه کاهش معنی‌داری در سطح اینترلوکین-6، TNF-α (با 0/001 > P) و hs-CRP(با 0/025 > P) و افزایش معنی‌دار میزان نشانگرهای التهابی- ایمنی سسیستماتیک (Systemic Immune-Inflammation Index=P × N/L) SIIi (0/001 > P) همراه بود.
نتیجه‌گیری: به نظر می‌رسد افزایش شدت وهله تمرین مقاومتی- با افزایش بار-  HLRTدر سیستم ایمنی اثر منفی نداشته و موجب کاهش فاکتورهای پیش‌التهابی اینترلوکین-6 و TNF-α و hs-CRP و همچنین افزایش SII که نشانه‌ی کاهش احتمال التهاب در زنان بدنساز می‌باشد.

تازه های تحقیق

رامین شعبانی: Google Scholar, PubMed 

علیرضا علمیه: PubMed, Google Scholar 

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The Effect of a Single Bout of Resistance Exercise with Two Intensities and Different Recovery on the Expression of TNF-Α and Interleukin-6 Genes, hs-CRP, and Systemic Immune-Inflammatory Marker SII on Female Bodybuilders

نویسندگان [English]

  • Azar Momtaz 1
  • Ramin Shabani 2
  • Alireza Elmieh 3
1 PhD Student in Exercise Physiology, Department of Physical Education and Sport Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran
2 Professor in Exercise Physiology, Department of Physical Education and Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran
3 Associated Professor in Exercise Physiology, Department of Physical Education and Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran
چکیده [English]

Background: Resistance exercises with high loads of HLRT cause physiological changes in body systems, including the immune system. On the other hand, little research has been done on the effect of high-intensity bodybuilding exercises on women's immune systems. The present study investigated the effect of resistance activity in a single bout with two different intensities and recovery on TNF-α and interleukin-6 gene expression, hs-CRP, and systemic immune-inflammatory marker SII on female bodybuilders.
Methods: In this semi-experimental study, 12 healthy female athletes (aged 30 ± 5) volunteered. The samples performed weekly resistance training at 70% and 85% of 1RM in two recovery times of one and two minutes. Thirty-six hours after training, sampling was done following a ten-hour fasting period. The samples were centrifuged at 3000 rpm and kept at -20 ℃. Ultimately, all levels of TNF-α and interleukin-6, hs-CRP, and systemic immune-inflammatory markers SII were checked in the medical laboratory. A consent form was obtained from the participants.
Findings: The results of resistance training using the HLRT method compared to moderate-intensity resistance training showed an increase in training intensity of up to 85% 1RM and a one-minute recovery. There was a significant decrease in the level of interleukin-6, TNF-α (P < 0.001), and hs-CRP (P < 0.025) and a significant increase in the level of SII systemic immune-inflammatory markers (P < 0.001).
Conclusion: It seems that increasing the intensity of resistance training - with increasing load - HLRT does not hurt the immune system, and it reduces pro-inflammatory factors interleukin-6, TNF-α and hs-CRP, as well as increases SII in female bodybuilders.

کلیدواژه‌ها [English]

  • Inflammation
  • Interleukin-6
  • hs-CRP
  • Resistance exercises
  • TNF-α

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مجله دانشکده پزشکی اصفهان
دوره 42، شماره 780
هفته 4، مهر
مهر و آبان 1403
صفحه 726-734

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سابقه مقاله

  • تاریخ دریافت: 01 اردیبهشت 1403
  • تاریخ پذیرش: 16 آبان 1403

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