نوع مقاله : مقاله های پژوهشی
نویسندگان
1 کارشناسی ارشد، گروه علوم تجربی، واحد دزفول، دانشگاه آزاد اسلامی، دزفول، ایران
2 استادیار، گروه علوم تجربی، واحد دزفول، دانشگاه آزاد اسلامی، دزفول، ایران
چکیده
تازه های تحقیق
آتوسا مرادزادگان: Google Scholar, PubMed
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Background: Stomach cancer is one of the most common malignancies worldwide, which leads to high mortality. The HMGA (high mobility group) family consists of chromatin structures that regulate proteins and play an meaningful role in tumorigenesis. This study was conducted to investigate the expression changes of these proteins in the patient population of Khuzestan, Iran.
Methods: In this survey, 60 tissue samples, including 30 gastric cancer tumor samples and 30 non-tumor samples (tumor margins), were prepared from the tumor tissue bank of Imam Khomeini Hospital (RA) in Ahvaz. After extracting RNA and evaluating its quantity and quality using a nanodrop device and agarose gel electrophoresis, the expression of HMGA-1 and HMGA-2 genes was assessed with a Real-time PCR technique. Statistical analysis was performed using t-test and ANOVA statistical methods.
Findings: The results showed that the relative expression of the HMGA-1 gene in tumor samples compared to non-tumor samples increased significantly by 17 times (P = 0.0001). Similarly, HMGA-2 gene expression in tumor samples significantly increased 15 times compared to ordinary samples (P = 0.0038).
Conclusion: This study points to the potential role of HMGA-1 and HMGA-2 in the development of gastric cancer in medical tumor samples in Khuzestan province, Iran. These findings align with previous research highlighting the roles of HMGA in various cancers, consistent with their potential role in tumorigenesis.
کلیدواژهها [English]