بررسی تأثیر اواریکتومی بر حافظه‌ی شناختی و یادگیری در موش‌های صحرایی مدل آلزایمری القا شده با اسکوپولامین

نوع مقاله : Original Article(s)

نویسندگان

1 کارشناسی ارشد، مرکز تحقیقات علوم اعصاب، دانشگاه علوم پزشکی ایران، تهران، ایران

2 دانشجو کارشناسی ارشد، کمیته‌ی تحقیقات دانشجویی، دانشگاه علوم پزشکی ایران، تهران، ایران

3 دانشجو دکتری، گروه آناتومی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی ایران، تهران، ایران

10.48305/jims.v42.i792.1027

چکیده

مقاله پژوهشی




مقدمه: بیماری آلزایمر (Alzheimer's disease) AD، یک بیماری پیشرونده و جزء شایع‌ترین اختلالات نورودژنراتیو می‌باشد. بیماری آلزایمر باعث اختلال در حافظه، یادگیری و سایر عملکردهای مهم ذهنی می‌شود. هدف این مطالعه، تأثیر اورایکتومی بر حافظه‌ی شناختی و یادگیری در مدل موش AD القا شده با اسکوپولامین می‌باشد.
روش‌ها: در مطالعه‌ی حاضر 32 موش صحرایی ماده نژاد ویستار (وزن 180 تا 200 گرم) در چهار گروه شاهد، اواریکتومی (OVX)، آلزایمری (AD) و اواریکتومی + آلزایمری (AD+OVX) تقسیم شد. مدل آلزایمری توسط تزریق اسکوپولامین، mg/kg 1 بصورت داخل صفاقی به مدت 14 روز القا شد. حافظه‌ی کوتاه‌مدت و یادگیری غیر اجتنابی به ترتیب توسط آزمون رفتاری ماز Y شکل و شاتل باکس ارزیابی شد. برای ارزیابی میزان تخریب نورونی در ناحیه‌ی CA1 نمونه‌های بافت هیپوکمپ از روش رنگ‌آمیزی نیسل استفاده گردید. داده‌ها توسط آزمون ANOVA یک‌طرفه و آزمون تقریبی Tukey در سطح معنی‌داری 05/0 آنالیز گردید.
یافته‌ها: بر طبق آزمون ماز، شاخص درصد تناوب در گروه OVX، AD و AD+OVX کاهش معنی‌داری نسبت به گروه شاهد مشاهده شد. بر اساس آزمون شاتل باکس، میانگین مدت ورود به ناحیه‌ی تاریک در گروه OVX، AD و AD+OVX کاهش معنی‌داری نسبت به گروه شاهد مشاهده شد. نتایج رنگ‌آمیزی نیسل نشان داد که میانگین تعداد سلول‌های زنده در گروه OVX، AD و AD+OVX نسبت به گروه شاهد کاهش معنی‌‌داری داشت.
نتیجه‌گیری: محرومیت از استروژن ناشی از OVX می‌تواند اثرات تشدید کننده‌ای در علایم ناشی از آلزایمر بر حافظه‌ی شناختی، یادگیری و همچنین آسیب نورونی داشته باشد.

تازه های تحقیق

رشاد رضاپور:  Google Scholar 

افسانه جابری اصل: Google Scholar, PubMed

کلیدواژه‌ها

عنوان مقاله [English]

Investigating the Effect of Ovariectomy on Cognitive Memory and Learning in Scopolamine-Induced Alzheimer's Model Rats

نویسندگان [English]

  • Reshad Rezapour 1
  • Hiva Osmani 2
  • Afsaneh Jaberi-Asl 3
1 MSc, Neuroscience Research Center, Iran University of Medical Sciences, Tehran, Iran
2 MSc Student, Student Research Committee, Iran University of Medical Sciences, Tehran, Iran
3 PhD Candidate, Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
چکیده [English]

Background: Alzheimer's disease (AD) is a progressive disease and one of the most common neurodegenerative disorders. AD causes impairment in memory, learning and other important mental functions. The aim of this study is the effect of ovariectomy on cognitive memory and learning in AD mouse model induced with scopolamine.
Methods: In the present study, 32 female Wistar rats (weight 180 to 200 grams) were divided into four groups: control, ovariectomy (OVX), Alzheimer's disease (AD), and ovariectomy + Alzheimer's disease (AD+OVX). Alzheimer's model was induced by intraperitoneal injection of scopolamine, 1 mg/kg for 14 days. Short-term memory and non-avoidance learning were evaluated by Y-shaped maze and shuttle box behavioral tests, respectively. Nissel's staining method was used to evaluate the degree of neuronal destruction in the CA1 area of hippocampal tissue samples. Data were analyzed by one-way ANOVA and Tukey's approximate test at a significance level of 0.05.
Findings: According to the Maze test, a significant decrease in frequency index was observed in the OVX, AD and AD+OVX groups compared to the control group. According to the shuttle box test, the average duration of entering the dark area in the OVX, AD and AD+OVX groups was significantly reduced compared to the control group. The Nissl staining results showed that the average number of live cells in the OVX, AD and AD+OVX groups was significantly reduced compared to the control group.
Conclusion: Deprivation of estrogen caused by OVX can have aggravating effects on Alzheimer's symptoms on cognitive memory, learning and also neuronal damage.

کلیدواژه‌ها [English]

  • Ovariectomy
  • Memory
  • Learning
  • Alzheimer's disease
  • Scopolamine

مراجع

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مجله دانشکده پزشکی اصفهان
دوره 42، شماره 792
هفته 4، دی
آذر و دی 1403
صفحه 1027-1036

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سابقه مقاله

  • تاریخ دریافت: 11 مرداد 1403
  • تاریخ پذیرش: 13 آذر 1403

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