نوع مقاله : ششمین کنگره غدد و متابولیسم
نویسندگان
1 گروه بیوتکنولوزی پزشکی
2 گروه بیوتکنولوژی پزشکی
3 گروه ببوتکنولوژی پزشکی
چکیده
تازه های تحقیق
امیرمسعودجلالوند: Google Scholar, PubMed
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Abstract
Background: The most important about the pathogenesis of idiopathic autoimmune diabetes is related to the presence of major autoantigens (1, 2). One of these important autoantigens that has been identified as targets of humoral autoimmunity in type 1 diabetes is insulin (3). Insulin autoantibodies are often the first autoantibodies to be detected in individuals with type 1 diabetes (4). The identification of insulin-specific epitopes recognized by T cells may contribute to the development of vaccines designed to prevent the autoimmune destruction of pancreatic β-cells in diabetes.
Method: The objective of the present investigation was to ascertain the most effective epitopes for the designing of a vaccine aimed at insulin, a significant autoantigen associated with type 1 diabetes mellitus. The UniProt database provided the amino acid sequence of insulin, which was used for epitope identification from the IEDB server. In the process of epitope identification, we employed the prevalent human alleles DRB1*01:01, DRB3*01:01, DRB4*01:01, and DRB5*01:01, each of which comprises a length of 15 base pairs.
Finding: We were able to find a common epitope for insulin by two IEDB servers at http://tools.iedb.org/mhcii/, TEPITOOL at http://tools.iedb.org/tepitool/. The sequence of this common epitope for insulin in these two servers was ERGFFYTPKTRREAE.
Conclusion: Since the development of vaccine design for high-prevalence diseases such as diabetes holds promise for the treatment and prevention of these diseases, in this study we decided to identify common epitopes associated with insulin, which is a key component in vaccine design.
کلیدواژهها [English]
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