مجله دانشکده پزشکی اصفهان

مجله دانشکده پزشکی اصفهان

ارتباط پلی‌مورفیسم TLR9-rs352140 و سطوح سرمی پروتئین واکنشگر-c، اینترلوکین-6 و IgG ضد-RBD با شانس ابتلا به سندرم کووید-19 طولانی

نوع مقاله : مقاله های پژوهشی

نویسندگان
زینب علوی تبار 1
حامد فولادسرشت 2
آمنه جاوید 3
انسیه ترکی 4
مجید حسین زاده 5
1 دانشجوی ژنتیک، گروه زیست‌شناسی، دانشکده‌ی فنی مهندسی و علوم، دانشگاه علم و هنر یزد، یزد، ایران
2 استادیار، گروه ایمنی‎ شناسی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران
3 استادیار، گروه زیست‌شناسی، دانشکده‌ی فنی مهندسی و علوم، دانشگاه علم و هنر یزد، یزد، ایران
4 کارشناسی ارشد، گروه ایمنی ‎شناسی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران
5 استادیار، گروه ژنتیک و بیولوژی مولکولی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران
10.48305/jims.v43.i814.0470
چکیده
مقدمه: سندروم کووید-19 طولانی (LCS: Long COVID-19 Syndrome) با علائم متنوع و طولانی، حداقل به مدت سه ماه پس از مرحله حاد کووید-19، بروز می‎کند. هنوز علل و عوامل خطر مرتبط با LCS بخوبی مشخص نشده ‎است. هدف از این مطالعه، بررسی ارتباط ویژگی‌های جمعیت‌شناختی، سابقه‌ی بستری، شاخص‌های آزمایشگاهی و پلی‌مورفیسم تک نوکلئوتیدی TLR9-rs352140 با شانس ابتلا به LCS بود.
روش‎ها: مشخصات جمعیت‌شناختی و سابقه ی بستری 88 فرد مبتلا به LCS (گروه LCS+) و 96 فرد بدون LCS (گروه LCS-) از مدارک پزشکی استخراج شد. روش الایزا به‌ منظور ارزیابی سطوح سرمی اینترلوکین-6 (IL-6) و IgG ضد دومن متصل شونده به پذیرنده (anti-RBD IgG) و روش ARMS-PCR برای تعیین پلی‌مورفیسم TLR9-rs352140 بکار گرفته شد.
یافته‎ها: فراوانی افراد با سابقه‌ی ابتلا به کووید-19 شدید و همچنین سطوح سرمی پروتئین واکنشگر -c (CRP) و IL-6 در گروه LCS+  بطور معنی‌داری بیشتر از گروه LCS- بود (به ترتیب 0/001 > P = 0/005  ،P و 0/001 > P). علاوه بر آن، فراوانی بیشتری از ژنوتایپ TT و آلل T پلی‌مورفیسم TLR9-rs352140 در گروه LCS+  مشاهده شد (به ترتیب 0/021 = P و 0/002 = P). در مقابل، سطح سرمی anti-RBD IgG و فراوانی ژنوتایپ CC و آلل C در گروه LCS+  کمتر از گروه LCS-  بود (به ترتیب 0/001 > P = 0/010  ،P و 0/002 = P). آزمون رگرسیون لجستیک چندگانه نشان داد که شانس ابتلا به LCS بطور معنی‌داری با سابقه‌ی ابتلا به کووید-19 شدید، سطوح‌ سرمی CRP و IL-6، و همچنین با آلل T پلی‌مورفیسم TLR9-rs352140 مرتبط است (به ترتیب 0/001 = P=0/002  ،P = 0/003 ،P و 0/018 = P).  درحالیکه، سطح سرمی anti-RBD IgG و فراوانی آلل C در ارتباط با محافظت در برابر LCS بود (به ترتیب 0/001 > P و 0/018 = P).
نتیجه‌گیری: سابقه‌ی ابتلا به کووید-19 شدید، سطوح سرمی IL-6 و CRP، و همچنین حضور آلل T پلی‌مورفیسم TLR9-rs352140 در ارتباط مستقیم با شانس ابتلا به LCS هستند.

تازه های تحقیق

زینب علوی تبار: Google Scholar, PubMed

حامد فولادسرشت: Google Scholar, PubMed

آمنه جاوید: Google Scholar, PubMed

انسیه ترکی: Google Scholar, PubMed

مجید حسین‌زاده: Google Scholar, PubMed

کلیدواژه‌ها
کووید طولانی
گیرنده‌ی شبه Toll نوع 9
پروتئین واکنشگر-c
اینترلوکین-6

موضوعات

عنوان مقاله English

Association of TLR9-rs352140 Polymorphism and Serum Levels of CRP, IL-6, and Anti-RBD IgG with the Chance of Developing Long COVID-19 Syndrome

نویسندگان English

Zeynab Alavitabar 1
Hamed Fouladseresht 2
Amaneh Javid 3
Ensiye Torki 4
Majid Hosseinzadeh 5
1 Genetics Student, Department of biological sciences, Faculty of Engineering and Science, Science and Art university of Yazd, Yazd, Iran
2 Assistant Professor, Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3 Assistant Professor, Department of biological sciences, Faculty of Engineering and Science, Science and Art university of Yazd, Yazd, Iran
4 MSc, Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
5 Assistant Professor, Department of Genetic and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
چکیده English

Background: Long COVID-19 syndrome (LCS) is characterized by a wide array of symptoms persisting for at least three months after the acute phase of COVID-19. The etiology and risk factors associated with LCS remain poorly understood. This study aimed to investigate the relationship between demographic characteristics, hospitalization history, laboratory parameters, and the TLR9-rs352140 single-nucleotide polymorphism in relation to the risk of developing LCS.
Methods: We collected demographic and hospitalization data from medical records of 88 individuals with LCS (LCS+ group) and 96 without LCS (LCS- group). Serum levels of interleukin-6 (IL-6) and anti-receptor binding domain IgG (anti-RBD IgG) were measured using ELISA, and the TLR9-rs352140 polymorphism was genotyped via ARMS-PCR.
Findings: The LCS+ group showed a significantly higher prevalence of severe COVID-19 history, along with elevated serum levels of C-reactive protein (CRP) and IL-6, compared to the LCS- group (P < 0.001, P = 0.005, and P < 0.001; respectively). Furthermore, the TT genotype and T allele of the TLR9-rs352140 polymorphism were more common in the LCS+ group (P = 0.021 and P = 0.002; respectively). Conversely, the serum level of anti-RBD IgG and the frequency of the CC genotype and C allele were lower (P < 0.001, P = 0.010, and P = 0.002; respectively). Multivariate logistic regression analysis indicated that a history of severe COVID-19, higher CRP and IL-6 levels, and the T allele of TLR9-rs352140 were significantly associated with an increased risk of LCS (P = 0.001, P = 0.003, P = 0.002, and P = 0.018; respectively). While, higher anti-RBD IgG levels and the C allele were linked to a reduced risk of LCS (P < 0.001 and P < 0.018; respectively).
Conclusion: A history of severe COVID-19, higher serum IL-6 and CRP levels, and the T allele of the TLR9-rs352140 polymorphism are positively correlated with the risk of developing LCS.

کلیدواژه‌ها English

Long COVID
Toll-Like Receptor 9
C-Reactive Protein
Interleukin-6
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مجله دانشکده پزشکی اصفهان
دوره 43، شماره 814
هفته 2، تیر
خرداد و تیر 1404
صفحه 470-480

  • تاریخ دریافت 23 اسفند 1403
  • تاریخ پذیرش 11 تیر 1404