مجله دانشکده پزشکی اصفهان

مجله دانشکده پزشکی اصفهان

تأثیر ترکیبی تمرینات تناوبی با شدت بالا (HIIT) و تمرین تداومی با شدت متوسط (MICT) بر پاسخ پروتئین‌های تانخورده میتوکندریایی (UPRmt) مرتبط با سیرتوئین در بطن چپ قلب رت‌‌های سالمند

نوع مقاله : مقاله های پژوهشی

نویسندگان
1 استادیار، گروه علوم بالینی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی آبادان، آبادان، ایران
2 استادیار، گروه تربیت بدنی و علوم ورزشی، واحد علی‌آباد کتول، دانشگاه آزاد اسلامی، علی‌آباد کتول، ایران
3 استادیار، گروه آموزش تربیت بدنی، دانشگاه فرهنگیان، صندوق پستی 889- 14665 تهران، ایران
10.48305/jims.v44.i853.0279
چکیده
مقدمه: هدف از مطالعه‌ی حاضر، تأثیر ترکیبی تمرینات هوازی با شدت بالا و متوسط بر پاسخ پروتئین‌های تانخورده میتوکندریایی (UPRmt) مرتبط با سیرتوئین در بطن چپ رت‌‌های سالمند بود.
روش‌ها: پژوهش تجربی- بنیادی حاضر شامل 12 سر رت نر 20 ماهه از نژاد ویستار با میانگین وزنی 30±400 گرم بود. معیار ورود رت‌های بالای 20 ماهگی بود. رت‌های مسن به طور تصادفی به گروه ترکیبی تمرینات تناوبی با شدت بالا (HIIT) و تمرین تداومی با شدت متوسط (MICT) (شش رت) و یک گروه کنترل (شش رت) تقسیم شدند. تمرینات ترکیبی HIIT و MICT هشت هفته‌ و هر هفته پنج جلسه انجام شد. برنامه‌ی HIIT با وهله‌های پرشدت (85-95 درصد سرعت بیشنیه) و استراحت (40-55 درصد) و برنامه MICT با شدت 40-70 درصد انجام شد.
یافته‌ها: بعد از هشت هفته تمرین ترکیبی هوازی میزان پروتئین سیرتوئین 3 (0/001 = P) و (0/001 = P) FOXO3a افزایش معناداری نسبت به گروه کنترل داشت. با این حال، انجام هشت هفته تمرین ترکیبی هوازی میزان پروتئین سیرتوئین 7 (0/25 = P) تغییر معناداری نداشت؛ ولی میزان پروتئین (0/007 = P) NRF1 افزایش معناداری را نشان داد. میزان (0/02 = P) SOD و کاتالاز (0/001 = P) در گروه تمرین ترکیبی هوازی افزایش معناداری را نسبت به گروه کنترل نشان داد.
نتیجه‌گیری: افزایش سطوح سیرتوئین 3، FOXO3A،NRF1 ، SOD و کاتالاز بر اهمیت ورزش هوازی برای افراد سالمند جهت سلامت سلولی و مقاومت در برابر استرس اکسیداتیو از طریق UPRmt تأکید می‌کند.

تازه های تحقیق

مهدی کیائی: Google Scholar , PubMed

مهدی مارزلو: Google Scholar , PubMed

حامد علیزاده پهلوانی: Google Scholar , PubMed

ندا آقایی بهمن بگلو: Google Scholar , PubMed

کلیدواژه‌ها
موضوعات

عنوان مقاله English

The combined effect of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on the sirtuin-related mitochondrial unfolded protein response (UPRmt) in the left ventricle of aged rats

نویسندگان English

Mehdi Kiaei 1
Mehdi Marezloo 2
Hamed Alizadeh Pahlavani 3
Neda Aghaei Bahmanbeglou 2
1 Department of Clinical Sciences, School of Medicine, Abadan University of Medical Sciences, Abadan, Iran
2 Department of Physical Education and Sport Sciences, AK.C, Islamic Azad University, Aliabad Katoul, Iran
3 Department of Physical Education, Farhangian University, P.O. Box 14665-889, Tehran, Iran
چکیده English

Background: The aim of the present study is to investigate the combined effect of high- and moderate-intensity aerobic training on the sirtuin-related mitochondrial unfolded protein response (UPRmt) in the left ventricle of aged rats.
Methods: The present experimental-basic study included 12 20-month-old male Wistar rats with an average weight of 400±30 g. The inclusion criteria were rats older than 20 months of age. The aged rats were randomly divided into a combined high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) group (six rats) and a control group (six rats). Combined HIIT and MICT training was performed for eight weeks, five sessions per week. The HIIT program was performed with high-intensity intervals (85-95% of maximum speed) and rest (40-55%), and the MICT program was performed with an intensity of 40-70%.
Findings: After eight weeks of combined aerobic training, the levels of sirtuin 3 (p=0.001) and FOXO3a (p=0.001) proteins significantly increased compared to the control group. However, after eight weeks of combined aerobic training, the levels of sirtuin 7 (p=0.25) proteins did not change significantly; however, the levels of NRF1 (p=0.007) proteins showed a significant increase. The levels of SOD (p=0.02) and catalase (p=0.001) in the combined aerobic training group showed a significant increase compared to the control group.
Conclusion: The increase in the levels of sirtuin 3, FOXO3A, NRF1, SOD and catalase emphasizes the importance of aerobic exercise for elderly people for cellular health and resistance to oxidative stress through UPRmt.

کلیدواژه‌ها English

Aging
high- and moderate-intensity aerobic exercise
Heart
Left ventricle
Unfolded protein response (UPRmt)
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دوره 44، شماره 853
هفته 1، اردیبهشت
فروردین و اردیبهشت 1405
صفحه 279-288

  • تاریخ دریافت 28 مرداد 1404
  • تاریخ پذیرش 25 اردیبهشت 1405