مجله دانشکده پزشکی اصفهان

مجله دانشکده پزشکی اصفهان

مروری بر فناوری‌‌های میکروسیال در حفظ باروری با تمرکز بر بافت تخمدان؛ شواهد بالینی و چشم‌اندازهای پژوهشی

نوع مقاله : Review Article

نویسندگان
1 گروه علوم تشریحی و بیولوژی تولید مثل، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران
2 دانشکده مهندسی شیمی، دانشگاه صنعتی اصفهان، اصفهان، ایران
10.48305/jims.v44.i866.1009
چکیده
مقدمه: فناوری‌ میکروسیال به ابزارهایی دگرگون‌کننده در پزشکی تولیدمثل تبدیل شده‌اند و با کنترل دقیق دینامیک سیالات در مقیاس میکرو، بازآفرینی شرایط فیزیولوژیک را برای جابه‌جایی گامت، کشت جنین و مدل‌سازی بافت تخمدانی ممکن می‌سازند.
روش‌ها: این مرور با تمرکز بر مقالات پر استناد ۲۰۱۵ تا ۲۰۲۵، چشم‌انداز کنونی پلتفرم‌های میکروسیال شامل سیستم‌های قطره‌محور، جریان پیوسته، چاپ سه‌بعدی و محیط متخلخل را بررسی می‌کند. همچنین در این مرور، بر انتخاب نوع مواد، شکل دستگاه‌ها و یکپارچه‌سازی عملکردی با تمرکز بر بافت تخمدان در عملکرد تکنیک‌های باروری مورد بحث و بررسی می‌باشد.
یافته‌ها: در تکنیک کمک‌باروری، جداسازی میکروسیالی اسپرم انتخاب اسپرماتوزوآی متحرک با DNA سالم و ریخت‌شناسی جنین را نشان داده است؛ اما بهبود پایدار در نرخ لقاح، بارداری بالینی و تولد زنده در جمعیت‌های انتخاب‌نشده اثبات ‌نشده مانده است. سیستم‌های Lab-on-a-chip که عریان‌سازی اووسیت، لقاح و کشت جنین را ترکیب می‌کنند، در مدل‌های حیوانی امکان‌پذیر بوده‌اند اما داده انسانی معتبر ندارند. در بافت شناسی تخمدان، پلتفرم‌های Follicle-on-a-chip از رشد فولیکول انسانی تحت پرفیوژن پشتیبانی می‌کنند و چیپ‌هایی مانند EVATAR پروفایل‌های هورمونی چرخه ۲۸ روزه را بازآفریده‌اند. کپسوله‌سازی Core-shell فولیکول‌زایی برون‌تنی را در موش تا اووسیت متافاز II پیش برده و راهبردهای حفظ باروری مستقل از پیوند را مطرح کرده است.
نتیجه‌گیری: موانع کلیدی مطالعات بالینی شامل مقیاس‌پذیری تولید، استانداردسازی، محدودیت نقاط پایانی انسانی و چالش‌های رگ‌زایی و کشت بلندمدت است. اولویت‌های آینده شامل کارآزمایی‌های تصادفی‌شده چندمرکزی با تولد زنده به‌عنوان نقطه پایانی اولیه، چارچوب‌های گزارش‌دهی استاندارد و تعامل با نهادهای نظارتی می‌شود.

تازه های تحقیق

حمید بهرامیان: Google ScholarPubMed

مهدی ستاری نجف آبادی: Google Scholar PubMed

حسین صالحی: Google ScholarPubMed

سید مهرداد عظیمی: Google ScholarPubMed

وجیهه عسگری: Google ScholarPubMed

کلیدواژه‌ها
موضوعات

عنوان مقاله English

A Review of Microfluidic Technologies in Fertility Preservation with a Focus on Ovarian Tissue: Clinical Evidence and Research Perspectives

نویسندگان English

Hamid Bahramian 1
Mehdi Sattari-Najafabadi 2
Hossein Salehi 1
Sayed Mehrdad Azimi 1
Vajihe Asgari 1
1 Department of Anatomical and Molecular Biology Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 Department of Chemical Engineering, Isfahan University of Technology, Isfahan, Iran
چکیده English

Background: Microfluidic technologies have emerged as transformative tools in reproductive medicine. By enabling precise control of fluid dynamics at the microscale, they make it possible to recreate physiological conditions for gamete handling, embryo culture, and ovarian tissue modeling.
Methods: This review draws on highly cited literature published between 2015 and 2025 to map the current landscape of microfluidic platforms. It examines droplet-based, continuous-flow, 3D-printed, and porous-media systems, with particular attention to material selection, device architecture, and functional integration with a deep focus on ovarian tissue in assisted reproductive Techniques.
Findings: In assisted reproductive Techniques, microfluidic sperm sorting has been shown to select motile spermatozoa with intact DNA and to improve embryo morphology. However, consistent gains in fertilization rate, clinical pregnancy, and live birth remain unproven in unselected populations. Lab-on-a-chip systems that combine oocyte denudation, fertilization, and embryo culture have proven feasible in animal models but lack robust human data. In ovarian biology, follicle-on-a-chip platforms support the growth of human follicles under perfusion, and chips such as EVATAR have reproduced the hormonal profiles of a 28-day cycle. Core-shell encapsulation has advanced in vitro folliculogenesis in mice through to the metaphase II oocyte stage, pointing toward transplantation-independent fertility-preservation strategies.
Conclusion: Key barriers to clinical investigations include manufacturing scalability, regulatory standardization, limited human endpoints, and the challenges of vascularization and long-term culture. Future priorities are multicenter randomized trials with live birth as the primary endpoint, standardized reporting frameworks, and early engagement with regulatory bodies.

کلیدواژه‌ها English

Microfluidics
Assisted Reproductive Techniques
Ovary
Lab-on-a-chip
Devices
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دوره 44، شماره 866
هفته 2، مرداد
مرداد و شهریور 1405
صفحه 1009-1024

  • تاریخ دریافت 17 خرداد 1405
  • تاریخ پذیرش 22 تیر 1405