نوع مقاله : مقاله های پژوهشی
1 دانشیلر، گروه زنان و زایمان، دانشکدهی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران
2 استادیار، گروه میکروبیولوژی، دانشکدهی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران
3 دستیار، گروه زنان و زایمان، دانشکدهی پزشکی و کمیتهی تحقیقات دانشجویی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران
عنوان مقاله [English]
Background: Cervical cancer is the second leading cause of mortality due to cancer among women. Since 1970, Human papillomavirus (HPV) has been proposed as the main etiologic factor for cervical cancer. High-Risk HPV types have been the major cause of cervical cancer. Therefore, it seems necessary that the most common HPV types in each population should be determined separately so that they can be used for an effective screening program, disease management, and the vaccination of the target population. To do so, the prevalence of type 6, 11, 16 and 18 of HPV were studied in married women aged 18-60 years, with normal Pap smear, referring to gynecology clinics in hospitals affiliated to Isfahan University of Medical Sciences, Iran, during 2009-2010 using the PCR technique.Methods: In this descriptive-analytical study, 180 married women aged 18-60 years with normal Pap smear, who referred to gynecology clinics in hospitals affiliated to Isfahan University of Medical Sciences, Iran, were selected through available sampling method. After cytological screening in Pap smear, a specimen was collected from all the normal samples using Cervex-Brush® Combi. DNA samples were extracted using phenol chloroform, and the extracted DNA samples were confirmed using PCO3 primers and PCO from Beta Globin gene. In the first stage of PCR, HPV was identified through primers GP5+ and GP6+, and the samples containing HPV underwent PCR again in order to determine their genotype using specific primers for types 6, 11, 16, and 18.Findings: In the first stage of PCR among 180 studied specimens, HPV was found in 46 specimens (25.55%). Out of 46 positive specimens, 7 specimens (15.21%) belonged to type 16, 6 specimens belonged to type 10 (13.04%), 18 specimens belonged to types 11 or 6 (21.74%), and 23 specimens (50%) belonged to other types of this virus. Among 180 studied samples, at least one of the high-risk types was found in 13 specimens (7.22%).Conclusion: Given to the high prevalence of this virus in the present study and other conducted studies, particularly types 16 and 18 which have been proved to be risk factors for premalignant and malignant cervical lesions, and also the good effect of Gardasil vaccine, using this vaccine can be an important step towards preventing cervical cancer in the studied women.