اثر عصاره‌ی گیاه بیلهر بر بروز پروتئین CD40 در آئورت سینه‌ای خرگوش‌های نر سفید تغذیه شده با رژیم پر کلسترول

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشیار، گروه علوم تشریحی و بیولوژی مولکولی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

2 کارشناس ارشد، گروه علوم تشریحی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی جهرم، جهرم، ایران

3 استاد، گروه فارماکوگنوزی، دانشکده‌ی داروسازی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

4 استادیار، گروه پاتولوژی، دانشکده‌ی دندان‌پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

چکیده

مقدمه: بیماری‌های شریان کرونر به دلیل تجمع رگه‌های چربی در انتیمای عروق اتفاق می‌افتند. امروزه نقش داروهای گیاهی در درمان بیماری‌ها بسیار قابل توجه است. این مطالعه با هدف بررسی میزان توانایی عصاره‌ی هیدروالکلی گیاه بیلهر بر کاهش بیان پروتئین CD40 در مقایسه با داروی استاتین صورت گرفت.روش‌ها: 30 خرگوش نر سفید (1250 تا 1500 گرم) از انستیتو پاستور تهران خریداری گردیدند و به 5 گروه 6 تایی تقسیم شدند. 4 گروه رژیم غذایی کلسترول 1 درصد و یک گروه رژیم معمولی بدون کلسترول دریافت کردند. یکی از 4 گروه تنها کلسترول 1 درصد و بقیه به ترتیب لووستاتین با دوز 6 میلی‌گرم به ازای هر کیلوگرم وزن بدن، عصاره‌ی گیاه بیلهر با دوز 200 میلی‌گرم به ازای هر کیلوگرم وزن بدن و عصاره‌ی گیاه بیلهر با دوز 400 میلی‌گرم به ازای هر کیلوگرم وزن بدن دریافت کردند.یافته‌ها: بررسی 30 مقطع تهیه شده از آئورت سینه‌ای خرگوش‌های مورد مطالعه، میزان رنگ‌پذیری متفاوتی را در تمام گروه‌ها نشان داد. گروه دریافت کننده‌ی عصاره‌ با دوز کم، تفاوت قابل توجهی با گروه دریافت کننده‌ی عصاره با دوز زیاد نداشت. گروه شاهد رنگ‌آمیزی بسیار ناچیز و در حد قابل چشم پوشی داشت.نتیجه‌گیری: عصاره‌ی گیاه بیلهر اثرات قابل توجهی در کاهش تولید پروتئین CD40 داشت. این خاصیت گیاه بیلهر می‌تواند به خاطر وجود فلاونوییدها و آنتی اکسیدان‌ها و سایر ترکیبات شناخته شده‌ی موجود در این گیاه نظیر فورانوکومارین‌ها باشد.

کلیدواژه‌ها

عنوان مقاله [English]

The Effect of Hydroalcoholic Extract of Dorema Aucheri on CD40 Protein Expression in Thoracic Aorta of Male White Rabbits Fed with Hypercholesterolemic Diet

نویسندگان [English]

  • Gholam Reza Dashti 1
  • Ahmad Teimourinejad 2
  • Mansour Salehi 1
  • Seyyed Ebrahim Sajadi 3
  • Nakisa Torabinia 4
1 Associate Professor, Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 Department of Anatomical Sciences, School of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran
3 Perofessor, Department of Pharmacognosy, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
4 Assistant Professor, Department of Pathology, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran
چکیده [English]

Background: Coronary artery disease (CAD) is caused by the accumulation of fatty streaks in the intima of vessels leading to atherosclerosis. Nowadays, medicinal herbs are attracting much attention in this field. Hence, the aim of the present research was to compare the effects of hydroalcoholic extract of the Dorema Aucheri and statins on CD40 protein expression.Methods: Thirty white male rabbits (1250-1500 grams) were purchased from the Pasteur Institute of Tehran. The animals were housed in clean metal hutches and acclimatized on rabbit chow for two weeks prior to the commencement of the experiment. The animals were subsequently randomly divided into five groups, each containing six rabbits. The feeding experiment was conducted at room temperature of 28˚C. After two weeks, four groups of rabbits were fed with a diet containing 1% cholesterol (hypercholesterolemic diet), hypercholesterolemic diet plus 6 mg/kg lovastatin, hypercholesterolemic diet plus 200 mg Dorema Aucheri extract, and hypercholesterolemic diet plus 400 mg Dorema Aucheri extract for 45 days. The fifth group only received a normal diet without cholesterol.Findings: Different levels of staining were observed in the 30 sections taken from the thoracic aorta of the rabbits. However, the two groups that received the extract were not significantly different. The staining was completely inconsiderable in the control. Conclusion: Dorema Aucheri extract had a significant effect on decreasing CD40 proteins formation. This property might be due to the presence of flavonoids, antioxidants and other compounds such as furanocoumarins that have been identified in the plant extract.

کلیدواژه‌ها [English]

  • Dorema aucheri
  • CD40
  • Cholesterol
  • Atherosclerosis
  • Lovastatin

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مجله دانشکده پزشکی اصفهان
دوره 29، شماره 166 - شماره پیاپی 166
بهمن و اسفند 1390
صفحه 2319-2329

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سابقه مقاله

  • تاریخ دریافت: 30 شهریور 1390
  • تاریخ بازنگری: 07 اردیبهشت 1403
  • تاریخ پذیرش: 17 فروردین 1401

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