ارتباط بین حضور توالی ویروس تومور پستان موش در بافت پستان و ابتلای به سرطان پستان در زنان ایرانی

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 کارشناس ارشد، گروه میکروبیولوژی، دانشگاه آزاد اسلامی، واحد قم، قم، ایران

2 استادیار، گروه میکروبیولوژی، دانشگاه آزاد اسلامی، واحد قم، قم، ایران

3 دانشیار، مرکز تحقیقات بیماری‌های کودکان و گروه علوم تشریحی و بیولوژی مولکولی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

چکیده

مقدمه: ویروس تومور پستان موش (Mouse mammary tumor virus یا MMTV) یک عامل پیشرفت تومورهای پستان در موش‌ها می‌باشد. مطالعات حضور توالی‌هایی مشابه سکانس‌های ویروسی MMTV را در نمونه‌های سرطان پستان در انسان نشان داده‌اند، اما به دلیل حضور رتروویروس‌های اندوژن انسانی (Human endogenous retroviruses یا HERVs)، نقش این ویروس هرگز به عنوان عامل تأثیرگذار در بروز سرطان پستان به اثبات نرسیده است. در این مطالعه، اثر MMTV بر سرطان پستان بررسی شد. برای غلبه بر تشابه توالی MMTV با وجود توالی‌های اندوژن، توالی‌هایی از ژن ENV-MMTV که همولوژی خیلی پایینی به HERV-Ks یا به هر ژن انسانی دیگری دارند، انتخاب شدند.روش‌ها: تحقیق حاضر به صورت مورد- شاهدی انجام شد. ابتدا DNA از 59 بافت توموری و شاهد سالم موجود در بلوک‌های پارافینی استخراج گردید. سپس از روش Nested PCR (Nested polymerase chain reaction) برای یافتن توالی‌های DNA مشابه سکانس‌های env ویروس MMTV استفاده شد.یافته‌ها: توالی‌های DNA مشابه سکانس‌های env ویروس MMTV در 19 نمونه (2/32 درصد) از 59 نمونه‌ی سرطان پستان یافت شدند، در صورتی که این توالی‌ها تنها در 3 نمونه‌ی بافت سالم پستان (5 درصد) مشاهده گردید (001/0 > P).نتیجه‌گیری: بر اساس نتایج به دست آمده، MMTV می‌تواند یکی از عوامل خطر مهم برای ایجاد سرطان پستان در بیماران ایرانی محسوب شود. تعیین توالی محصول PCR نشان داد که این توالی‌ها 99 درصد با MMTV سویه‌ی موشی BR6 و 100 درصد با سویه‌های موشی C3H و GR همولوژی دارند.

کلیدواژه‌ها

عنوان مقاله [English]

Relation between the Presence of Mouse Mammary Tumor Virus-Like Sequences in Breast Tissues and Human Breast Cancer in Iranian Patients

نویسندگان [English]

  • Seyed Shervin Shariatpanahi 1
  • Mohammad Soleimani Darjagh 2
  • Rasoul Salehi 3
1 Department of Microbiology, Islamic Azad University, Qom Branch, Qom, Iran
2 Assistant Professor, Department of Microbiology, Islamic Azad University, Qom Branch, Qom, Iran
3 Associate Professor, Pediatric Diseases Research Center AND Department of Anatomy and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
چکیده [English]

Background: Mouse mammary tumor virus (MMTV), a milk-transmitted retrovirus, is considered to be involved in the development of breast tumors in mice. Although several previous studies have identified MMTV-like sequences in human breast cancer, the presence of human endogenous retrovirus (HERs) makes these results controversial, i.e. the role of MMTV in breast cancer has never been definitely proven. In addition, various results have been reported in this regard from different countries. We selected MMTV envelope (Env) gene sequences to eliminate the effects of HERs.Methods: In this case-control study, DNA was extracted from 59 paraffin-embedded malignant tumor tissues and 59 normal control samples. Nested polymerase chain reaction (PCR) was then used to detect MMTV Env-like sequences.Findings: MMTV genome was detected in 19 malignant breast samples (32%) and three normal control samples (5%) (P < 0.001).Conclusion: Based on our findings, MMTV can play a major role in the development of breast cancer in Iranian women. According to PCR results, the obtained sequences had 99% homology with BR6 strain of MMTV and 100% homology with GR and C3H strains.

کلیدواژه‌ها [English]

  • Mouse mammary tumor virus
  • Breast Cancer
  • Malignant tumors
  • Risk Factors
  • Nested polymerase chain reaction

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مجله دانشکده پزشکی اصفهان
دوره 31، شماره 227 - شماره پیاپی 227
فروردین و اردیبهشت 1392
صفحه 208-217

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سابقه مقاله

  • تاریخ دریافت: 22 بهمن 1391
  • تاریخ بازنگری: 31 فروردین 1403
  • تاریخ پذیرش: 17 فروردین 1401

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