Journal of Isfahan Medical School

Journal of Isfahan Medical School

Effect of Extremely Low-Frequency (50 Hz) Field on Proliferation Rate of Human Adipose-Derived Mesenchymal Stem Cells

Document Type : Original Article(s)

Authors
Marzieh Salimi 1
Daryoush Shahbazi-Gahrouei 2
Saeid Karbasi 3
Saied Kermani 3
Shahnaz Razavi 4
1 MSc Student, Department of Medical Physics and Medical Engineering, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran
2 Professor, Department of Medical Physics and Medical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3 Associate Professor, Department of Medical Physics and Medical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
4 Associate Professor, Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract
Background: The effects of non-ionizing extremely low-frequency electromagnetic fields (ELF-EMF) chronic exposure on human beings due to its potential health hazards has become a focus of interest since many years ago. Stem cells are useful models for assessment of effects of ELF-EMF on other cell lines and human beings. Adipose tissue has been known source of multipotent stromal cells (MSCs), which can be obtained by a less invasive method and in large amounts compared with bone marrow stromal cells (BMSCs); so this study was done on human adipose-derived stem cells (hADSCs). The effect of ELF-EMF with intensity of 0.5 and 1 mT and 50 Hz on proliferation rates of hADSCs at 20 and 40 min/day for 7 days was assessed.Methods: MTT assay was used to determine the growth and metabolism of cells and Trypan blue test was also done for cell viability.Findings: The proliferation rate and growth of hADSCs in all exposure groups was significantly higher than that in sham groups except in group of 1 mT, 40 min/day (P < 0.05).Conclusion: The results showed that 0.5 and 1 mT magnetic strength fields can promote the proliferation rates of the hMSCs derived adipose tissue regarding the duration of exposure.
Keywords
Non-ionizing extremely low-frequency electromagnetic fields (ELF-EMF)
Human adipose-derived stem cells (hADSCs)
Adipose
Proliferation rate
Growth
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Journal of Isfahan Medical School
Volume 31, Issue 232 - Serial Number 232
March and April 2013
Pages 439-455

  • Receive Date 17 April 2013
  • Accept Date 06 April 2022