نوع مقاله : مقاله های پژوهشی
1 کارشناس ارشد، گروه میکروبیولوژی، دانشگاه آزاد اسلامی، واحد فلاورجان، اصفهان، ایران
2 استاد، مرکز تحقیقات غدد و متابولیسم، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران
3 دانشیار، گروه ژنتیک و بیولوژی مولکولی، دانشکدهی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران
عنوان مقاله [English]
Background: Obesity and Diabetes are two metabolic diseases that are dependent on genetic and various environmental factors. Today, obesity and type 2 diabetes cannot just be attributed to genome changes, bad eating habits, or physical activity reduction. Gut microflora is another effective factor that has an important role in health. Recently, numerous experiments have been done to investigate the relation between metabolic diseases and bacterial populations in the gut. The purpose of this study is to assess the bifidobacteria of the intestinal microbiota in people with type 2 diabetes and compare with healthy persons.Methods: In this study, 40 male and female adults, 20 of whom were diagnosed with type 2 diabetes, were studied. For this study, age, gender, and body-mass index (BMI) are matched in diabetic and healthy persons. After fecal sample collection from diabetic and healthy persons, and extraction of DNA from samples, culture and extraction of DNA from standard bifidobacteria were done as a positive control. After that, cloning in PTZ57 R/T plasmid and extraction cloning plasmid was done. For quantitative investigation of bifidobacteria, Real-time PCR molecular technique was done by using special primer of bifidobacterium and standard curve. To study the number of bifidobacteria in people with type 2 diabetes and compare with healthy persons, statistical analysis was done.Findings: Average number of bacteria in people with type 2 diabetes is more than in healthy persons, but Student’s independent t-test) P = 0.09) and Mann-Whitney test (P = 0.1) indicate that there are no meaningful differences between them.Conclusion: Quantitative calculation with Real-time PCR indicates that the number of bifidobacteria is not effective in Iranian type 2 diabetes patients.